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Mouse Anti-FGFR4 Recombinant Antibody (clone LD1)

CAT#: NS-028CN

Anti-FGFR4 antibody was found to bind mouse complement protein C3 and can be used to inhibit cancer.

Published Data Gene Expression

Figure 1 LD1 binds to human (h), mouse (m), and cynomolgus monkey (c) FGFR4, but does not bind to hFGFR1, hFGFR2, or hFGFR3.

ELISA

Figure 1 LD1 binds to human (h), mouse (m), and cynomolgus monkey (c) FGFR4, but does not bind to hFGFR1, hFGFR2, or hFGFR3.

The binding of LD1 to immobilized FGFR-Fc chimeric proteins was determined by solid phase binding assay.

French, D. M., Lin, B. C., Wang, M., Adams, C., Shek, T., Hötzel, K.,... & Rodriguez, L. A. (2012). Targeting FGFR4 inhibits hepatocellular carcinoma in preclinical mouse models. PloS one, 7(5).

Figure 2 Binding of LD1 to hFGFR4 expressed at the cell surface of stably transfected HEK293 cells as measured by FACS (RFU = Relative Fluorescence Unit).

FC

Figure 2 Binding of LD1 to hFGFR4 expressed at the cell surface of stably transfected HEK293 cells as measured by FACS (RFU = Relative Fluorescence Unit).

French, D. M., Lin, B. C., Wang, M., Adams, C., Shek, T., Hötzel, K.,... & Rodriguez, L. A. (2012). Targeting FGFR4 inhibits hepatocellular carcinoma in preclinical mouse models. PloS one, 7(5).

Figure 3 The binding of LD1 to immobilized hFGFR4-Flag chimeric proteins bearing point mutations as measured by a solid phase binding assay.

ELISA

Figure 3 The binding of LD1 to immobilized hFGFR4-Flag chimeric proteins bearing point mutations as measured by a solid phase binding assay.

French, D. M., Lin, B. C., Wang, M., Adams, C., Shek, T., Hötzel, K.,... & Rodriguez, L. A. (2012). Targeting FGFR4 inhibits hepatocellular carcinoma in preclinical mouse models. PloS one, 7(5).

Figure 4 The binding of LD1 to hFGFR4-Flag chimeric proteins bearing point mutations as evaluated by Western blot.

WB

Figure 4 The binding of LD1 to hFGFR4-Flag chimeric proteins bearing point mutations as evaluated by Western blot.

Mutated proteins were electrophoresed and sequentially immunoblotted using LD1, an anti-FGFR4 (8G11), and an anti-Flag antibody.

French, D. M., Lin, B. C., Wang, M., Adams, C., Shek, T., Hötzel, K.,... & Rodriguez, L. A. (2012). Targeting FGFR4 inhibits hepatocellular carcinoma in preclinical mouse models. PloS one, 7(5).

Figure 5 A, LD1 inhibits FGFR4 binding to FGF1 and FGF19 as determined by solid phase binding assay. B, LD1 inhibits FGF1-stimulated proliferation of BaF3 cells stably expressing FGFR4/R1. C, LD1 inhibits FGFR4 signaling in L6 cells stably expressing FGFR4. D, Cell surface expression of FGFR4 protein in a subset of liver tumor cell lines as determined by FACS analysis using LD1.

Inhib

Figure 5 A, LD1 inhibits FGFR4 binding to FGF1 and FGF19 as determined by solid phase binding assay. B, LD1 inhibits FGF1-stimulated proliferation of BaF3 cells stably expressing FGFR4/R1. C, LD1 inhibits FGFR4 signaling in L6 cells stably expressing FGFR4. D, Cell surface expression of FGFR4 protein in a subset of liver tumor cell lines as determined by FACS analysis using LD1.

French, D. M., Lin, B. C., Wang, M., Adams, C., Shek, T., Hötzel, K.,... & Rodriguez, L. A. (2012). Targeting FGFR4 inhibits hepatocellular carcinoma in preclinical mouse models. PloS one, 7(5).

Figure 6 A, LD1 inhibits FGFR4 signaling in HEP3B cells as evaluated by Western blot. B, LD1 inhibits the FGFR4-regulated CYP7A1 repression in HEP3B cells. CYP7A1 levels are represented as fold expression relative to the level in untreated cells. C, LD1 inhibits FGFR4-regulated FOS expression in a panel of liver cancer cell lines. The results are represented as fold expression relative to the FOS level in untreated cells. D, Inhibition of colony formation by repression of FGFR4 expression in JHH5 cells stably transfected with an FGFR4 shRNA doxycycline-inducible vector. E, Enumeration of LD1-inhibited liver cancer cell line colony formation. The values are represented as percent of the number of colonies enumerated in the absence of added LD1. F, LD1 inhibits HCC cell line colony formation.

Inhib

Figure 6 A, LD1 inhibits FGFR4 signaling in HEP3B cells as evaluated by Western blot. B, LD1 inhibits the FGFR4-regulated CYP7A1 repression in HEP3B cells. CYP7A1 levels are represented as fold expression relative to the level in untreated cells. C, LD1 inhibits FGFR4-regulated FOS expression in a panel of liver cancer cell lines. The results are represented as fold expression relative to the FOS level in untreated cells. D, Inhibition of colony formation by repression of FGFR4 expression in JHH5 cells stably transfected with an FGFR4 shRNA doxycycline-inducible vector. E, Enumeration of LD1-inhibited liver cancer cell line colony formation. The values are represented as percent of the number of colonies enumerated in the absence of added LD1. F, LD1 inhibits HCC cell line colony formation.

French, D. M., Lin, B. C., Wang, M., Adams, C., Shek, T., Hötzel, K.,... & Rodriguez, L. A. (2012). Targeting FGFR4 inhibits hepatocellular carcinoma in preclinical mouse models. PloS one, 7(5).

❮

Figure 1 LD1 binds to human (h), mouse (m), and cynomolgus monkey (c) FGFR4, but does not bind to hFGFR1, hFGFR2, or hFGFR3.

Figure 2 Binding of LD1 to hFGFR4 expressed at the cell surface of stably transfected HEK293 cells as measured by FACS (RFU = Relative Fluorescence Unit).

Figure 3 The binding of LD1 to immobilized hFGFR4-Flag chimeric proteins bearing point mutations as measured by a solid phase binding assay.

Figure 4 The binding of LD1 to hFGFR4-Flag chimeric proteins bearing point mutations as evaluated by Western blot.

Figure 5 A, LD1 inhibits FGFR4 binding to FGF1 and FGF19 as determined by solid phase binding assay. B, LD1 inhibits FGF1-stimulated proliferation of BaF3 cells stably expressing FGFR4/R1. C, LD1 inhibits FGFR4 signaling in L6 cells stably expressing FGFR4. D, Cell surface expression of FGFR4 protein in a subset of liver tumor cell lines as determined by FACS analysis using LD1.

Figure 6 A, LD1 inhibits FGFR4 signaling in HEP3B cells as evaluated by Western blot. B, LD1 inhibits the FGFR4-regulated CYP7A1 repression in HEP3B cells. CYP7A1 levels are represented as fold expression relative to the level in untreated cells. C, LD1 inhibits FGFR4-regulated FOS expression in a panel of liver cancer cell lines. The results are represented as fold expression relative to the FOS level in untreated cells. D, Inhibition of colony formation by repression of FGFR4 expression in JHH5 cells stably transfected with an FGFR4 shRNA doxycycline-inducible vector. E, Enumeration of LD1-inhibited liver cancer cell line colony formation. The values are represented as percent of the number of colonies enumerated in the absence of added LD1. F, LD1 inhibits HCC cell line colony formation.

❯

Figure 1 IHC staining of human smooth muscle

Normal Tissue Figure 1 IHC staining of human smooth muscle

Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27273/ihc_selected.jpg

Figure 2 IHC staining of human gall bladder

Normal Tissue Figure 2 IHC staining of human gall bladder

Immunohistochemical staining of human gall bladder shows strong cytoplasmic and membrane positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28251/ihc_selected.jpg

Figure 3 IHC staining of human prostate

Normal Tissue Figure 3 IHC staining of human prostate

Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5196/ihc_selected.jpg

Figure 4 Colon

Normal Tissue Figure 4 Colon

Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28251/61139_A_9_3.jpg

Figure 5 Liver

Normal Tissue Figure 5 Liver

Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28251/61139_A_9_4.jpg

Figure 6 Kidney

Normal Tissue Figure 6 Kidney

Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28251/61139_A_7_5.jpg

Figure 7 Testis

Normal Tissue Figure 7 Testis

Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28251/61139_A_4_6.jpg

Figure 8 RNA cell line category: Group enriched (CACO-2, Hep G2, RH-30)

RNA Expression Figure 8 RNA cell line category: Group enriched (CACO-2, Hep G2, RH-30)

Cell lines ordered by descending RNA expression order

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000160867-FGFR4

❮

Figure 1 IHC staining of human smooth muscle

Figure 2 IHC staining of human gall bladder

Figure 3 IHC staining of human prostate

Figure 6 Kidney

Figure 7 Testis

Figure 8 RNA cell line category: Group enriched (CACO-2, Hep G2, RH-30)

❯

Specifications

Immunogen
Recombinant human FGFR4-Fc chimeric proteins

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human FGFR4

Species Reactivity
Human

Clone
LD1

Applications
ELISA, WB, FC, IHC, IP

Related Disease
Cancer

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Inhibition.

Target

Alternative Names
Fibroblast Growth Factor Receptor 4; EC 2.7.10.1; JTK2; TKF; Tyrosine Kinase Related To Fibroblast Growth Factor Receptor; Hydroxyaryl-Protein Kinase; Protein-Tyrosine Kinase; Tyrosylprotein Kinase; CD334 Antigen; EC 2.7.10; FGFR-4; CD334

Gene ID
2264

UniProt ID
P22455

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Related Diseases

EGFR Tyrosine Kinase Inhibitor Resistance

EGFR Tyrosine Kinase Inhibitor Resistance

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - NS-028CN.pdf

MSDS

MSDS - NS-028CN.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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CAT
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TAB-066WM-F(E)	Anti-Human FGFR4 Recombinant Antibody Fab Fragment (XPA.48.056)	ELISA, FC	Human Antibody
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MOR-1300	Hi-Affi™ Rabbit Anti-FGFR4 Recombinant Antibody (clone DS1300AB)	WB	Rabbit IgG
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CAT	Product Name	Application	Type
HPAB-0054-LS-S(P)	Human Anti-FGFR4 Recombinant Antibody; scFv Fragment (HPAB-0054-LS-S(P))	Inhib	Human scFv
HPAB-0055-LS-S(P)	Human Anti-FGFR4 Recombinant Antibody; scFv Fragment (HPAB-0055-LS-S(P))	Inhib	Human scFv
HPAB-0056-LS-S(P)	Human Anti-FGFR4 Recombinant Antibody; scFv Fragment (HPAB-0056-LS-S(P))	Inhib	Human scFv
HPAB-2922LY-S(P)	Mouse Anti-FGFR4 Recombinant Antibody; scFv Fragment (HPAB-2922LY-S(P))	ELISA, FC	Mouse scFv
HPAB-0442-FY-S(P)	Human Anti-FGFR4 Recombinant Antibody; scFv Fragment (HPAB-0442-FY-S(P))	Inhib	Human scFv

CAT	Product Name	Application	Type
HPAB-0054-LS-F(E)	Human Anti-FGFR4 Recombinant Antibody; Fab Fragment (HPAB-0054-LS-F(E))	Inhib	Human Fab
HPAB-0055-LS-F(E)	Human Anti-FGFR4 Recombinant Antibody; Fab Fragment (HPAB-0055-LS-F(E))	Inhib	Human Fab
HPAB-0056-LS-F(E)	Human Anti-FGFR4 Recombinant Antibody; Fab Fragment (HPAB-0056-LS-F(E))	Inhib	Human Fab
HPAB-2922LY-F(E)	Mouse Anti-FGFR4 Recombinant Antibody; Fab Fragment (HPAB-2922LY-F(E))	ELISA, FC	Mouse Fab
HPAB-0442-FY-F(E)	Human Anti-FGFR4 Recombinant Antibody; Fab Fragment (HPAB-0442-FY-F(E))	Inhib	Human Fab

CAT	Product Name	Application	Type
NS-028CN-F(E)	Mouse Anti-FGFR4 Recombinant Antibody (clone LD1); Fab Fragment	ELISA, WB, FC, IHC, IP	Mouse Fab
NS-028CN-S(P)	Mouse Anti-FGFR4 Recombinant Antibody (clone LD1); scFv Fragment	ELISA, WB, FC, IHC, IP	Mouse scFv

CAT	Product Name	Application	Type
MHC-YF433	A*02:01/Human FGFR4 (ALLAGLVSL) MHC Pentamer	FCM

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CAT	Product Name	Application	Type
VS-0525-XY2545	Anti-FGFR4 Immunohistochemistry Kit	IHC

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