Recombinant Mouse Antibody (3F8) is capable of binding to GD2 ganglioside, expressed in Chinese Hamster Ovary cells (CHO). It is a murine IgG3 with a moderate affinity for GD2 (KD = 5 nM).
Figure 1 Molecular Dynamics Analyses of Unliganded 3F8
(A and B)Worm plots of germline 3F8 (A) and affinitymature 3F8 (B) illustrating a relatively rigid germline structure that loosens slightly during affinity maturation. CDR loops are labeled, and residuemutations between germline and affinity-mature antibodies are shown as stick representation. (C–H) Histograms of RMSD distribution from the starting conformation for CDR loops during the simulations. Light-chain CDRs 1–3 (C–E, respectively) and heavy-chain CDRs 1–3 (F–H, respectively) illustrate the propensity of mature CDRs to explore the conformational space more liberally.
Figure 2 3F8 Antibody Direct Cytotoxicity.
The 3F8 antibodies (mature and germline) were capable of promoting direct cytotoxicity against the GD2-presenting M14 melanoma cell lines. These antibodies were incapable of inducing direct cytotoxicity against the colorectal cancer cell line SW620.
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