Mouse Anti-GD2 Recombinant Antibody (clone ME36.1) (CAT#: PABZ-049)
Recombinant Mouse Antibody (ME36. 1) is capable of binding to GD2, expressed in Chinese Hamster Ovary cells (CHO). The ME36. 1 monoclonal antibody binds to the GD2 ganglioside and has shown potential as a therapeutic antibody. ME36. 1 is a possible alternative therapy to radiation, which is often ineffective in late-stage melanoma.
Specific Inquiry
Figure 1 Reactivity of ME36.1 at 4 μg/mL overlaid on varying amounts of standard GD3 (A) and GD2 (B).
Second antibody, ¹²⁵I labeled goat anti-mouse IgG, was used at 10⁶ cpm/mL. Radioactivity was counted after removal of relevant TLC areas as described. Gangliosides are quantitated and expressed as nanograms of total molecule.
Sela, B. A., Iliopoulos, D., Guerry, D., Herlyn, D., & Koprowski, H. (1989). Levels of Disialogangliosides in Sera of Melanoma Patients Monitored by Sensitive ThinLayer Chromatography and Immunostaining. JNCI: Journal of the National Cancer Institute, 81(19), 1489-1492.
Figure 2 Levels of GD2 and GD3 extracted from sera of 15 normal donors and 15 melanoma patients.
Concentration of gangliosides is based on titration of their known quantities in overlay assay with ME36.1, as shown in fig. 1. GD2 and GD3 in serum samples from patient No. 13 are represented before (upper asterisk-labeled dots in both panels) and after (lower asterisk-labeled dots) immunotherapy with ME36.1.
Sela, B. A., Iliopoulos, D., Guerry, D., Herlyn, D., & Koprowski, H. (1989). Levels of Disialogangliosides in Sera of Melanoma Patients Monitored by Sensitive ThinLayer Chromatography and Immunostaining. JNCI: Journal of the National Cancer Institute, 81(19), 1489-1492.
Figure 3 Reactivity of ME36.1 with GD2 and GD3 in sera from melanoma patients.
Immunostaining of gangliosides separated on TLC. Lanes A-E, gangliosides extracted from sera of normal donors; lanes F and G, those from patient No. 13 before and after treatment with ME36.1, respectively; lanes H-O, gangliosides from melanoma patients in active phase of disease. Each lane represents gangliosides extracted from 60 μL of serum.
Sela, B. A., Iliopoulos, D., Guerry, D., Herlyn, D., & Koprowski, H. (1989). Levels of Disialogangliosides in Sera of Melanoma Patients Monitored by Sensitive ThinLayer Chromatography and Immunostaining. JNCI: Journal of the National Cancer Institute, 81(19), 1489-1492.
Specifications
- Immunogen
- Melanoma GD2 ganglioside
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human GD2
- Species Reactivity
- Human
- Clone
- ME36.1
- Applications
- WB, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody ME36.1 has been reported in applications of Functional Assay, Western Blot. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
- Alternative Names
- disialoganglioside; GD2; GD2 ganglioside
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-770-sdAb | Recombinant Anti-Human GD2 VHH Single Domain Antibody | Neut, ChiP, IP, FUNC | Llama VHH |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-731 | Anti-GD2 Recombinant Antibody (Dinutuximab) | Neut, ELISA, IF, IP, FuncS, FC, ICC | IgG1 - kappa |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L018R | anti-GD2 immunotoxin 14G2a (IgG)-RTA | Cytotoxicity assay, Functional assay |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-304CL | Anti-Human GD2 Recombinant Antibody (hu14.18K322A) | FC, FuncS | Antibody |
| PABX-059 | Human Anti-GD2 Recombinant Antibody (PABX-059) | FuncS | IgG1, κ |
| PABX-059-F (E) | Human Anti-GD2 Recombinant Antibody; Fab Fragment (PABX-059-F (E)) | FC, ELISA, FuncS | Human Fab |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-100 | Mouse Anti-GD2 Recombinant Antibody (clone 14G2a); Fab Fragment | ELISA, WB, FuncS | Mouse Fab |
| PFBZ-049 | Mouse Anti-GD2 Recombinant Antibody (clone ME36.1); Fab Fragment | WB, FuncS | Mouse Fab |
| FAMAB-0126-YC-F(E) | Mouse Anti-GD2 Recombinant Antibody (clone 7A4); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0027-WJ-F(E) | Mouse Anti-GD2 Recombinant Antibody; Fab Fragment (HPAB-0027-WJ-F(E)) | ELISA, RIA | Mouse Fab |
| HPAB-1030WJ-F(E) | Human Anti-GD2 Recombinant Antibody; Fab Fragment (HPAB-1030WJ-F(E)) | ELISA | Chimeric (mouse/human) Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-049 | Mouse Anti-GD2 Recombinant Antibody (clone ME36.1); scFv Fragment | WB, FuncS | Mouse scFv |
| MOB-181CQ | Human Anti-GD2 Recombinant Antibody (MOB-181CQ) | ELISA, FC, FuncS | Humanized scFv |
| HPAB-0701-CN-S(P) | Human Anti-GD2 Recombinant Antibody (clone AB527); scFv Fragment | ELISA, FC, IHC, IF | Human scFv |
| HPAB-1524WJ-S(P) | Human Anti-GD2 Recombinant Antibody; scFv Fragment (clone KM8138) | ELISA, WB | Human scFv |
| HPAB-1525WJ-S(P) | Human Anti-GD2 Recombinant Antibody; scFv Fragment (clone KM1138) | ELISA, WB | Human scFv |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-074CQ | Human Anti-GD2 Recombinant Antibody (TAB-074CQ) | ELISA, FC | Human IgG1, κ |
| TAB-075CQ | Human Anti-GD2 Recombinant Antibody (TAB-075CQ) | ELISA, FC | Human IgM, κ |
| TAB-076CQ | Human Anti-GD2 Recombinant Antibody (TAB-076CQ) | ELISA, FC | Human IgG1, κ |
| TAB-077CQ | Human Anti-GD2 Recombinant Antibody (TAB-077CQ) | ELISA, FC | Human IgG1, κ |
| PABX-059-S (P) | Human Anti-GD2 Recombinant Antibody; scFv Fragment (PABX-059-S (P)) | FC, ELISA, FuncS | scFv |
Neuroscience Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-029CN | Mouse Anti-GD2 Recombinant Antibody (clone 5F11) | WB | Mouse IgG |
| NS-030CN | Mouse Anti-GD2 Recombinant Antibody (clone 3G6) | WB | Mouse IgG |
| NS-029CN-F(E) | Mouse Anti-GD2 Recombinant Antibody (clone 5F11); Fab Fragment | WB | Mouse Fab |
| NS-030CN-F(E) | Mouse Anti-GD2 Recombinant Antibody (clone 3G6); Fab Fragment | WB | Mouse Fab |
| NS-029CN-S(P) | Mouse Anti-GD2 Recombinant Antibody (clone 5F11); scFv Fragment | WB | Mouse scFv |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0126YC | Mouse Anti-GD2 Recombinant Antibody (clone 7A4) | ELISA | Mouse IgG3, κ |
| HPAB-0701-CN | Human Anti-GD2 Recombinant Antibody (clone AB527) | ELISA, FC, IHC, IF | Human IgM |
| HPAB-0027-WJ | Mouse Anti-GD2 Recombinant Antibody (HPAB-0027-WJ) | ELISA, RIA | Mouse IgG3, κ |
| HPAB-1524WJ | Human Anti-GD2 Recombinant Antibody (clone KM8138) | ELISA, WB | Human IgG |
| HPAB-1525WJ | Human Anti-GD2 Recombinant Antibody (clone KM1138) | ELISA, WB | Human IgG |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-731 | Afuco™ Anti-GD2 ADCC Recombinant Antibody (Dinutuximab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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