Mouse Anti-GD2 Recombinant Antibody (clone 3G6)
CAT#: NS-030CN
A recombinant antibody construct comprises the variable regions of the heavy and light chain of an anti-GD2 antibody.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human GD2
- Species Reactivity
- Human
- Clone
- 3G6
- Applications
- WB
- Related Disease
- Tumor
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Analysis of cell surface GD2, complement inhibitor expression, and C3 deposition was done as described. For analysis of mouse C3 deposition on EL4 cells in vitro, cells (5 × 10⁵) were resuspended in 20 mL PBS or anti-GD2 mAb (either 14G2a or 3G6) at 50 mg/ml and incubated for 30 minutes at 4°C. After washing, cells were resuspended in 20 mL PBS or complement inhibitor blocking mAb (5D5 and/or 3B3; final concentration, 50 mg/ml) and incubated at 4°C for 30 minutes. Cells were then washed and incubated in 30 mL of 30% mouse serum diluted in gelatin veronal-buffered saline (GVB++; Sigma) at 37°C for 30 minutes. Cells were then washed with EDTA-GVB, incubated with FITC-conjugated goat anti-mouse C3 (30 minutes/4°C), and washed twice. Finally, cells were suspended in PBS containing 2 mg/ml propidium iodide and analyzed by flow cytometry. Propidium iodide–positive (dead) cells were excluded when fluorescence intensity was calculated.
Target
- Alternative Names
- GD2 ganglioside; GD2
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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- Product Name
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| NS-029CN-F(E) | Mouse Anti-GD2 Recombinant Antibody (clone 5F11); Fab Fragment | WB | Mouse Fab |
| CAT | Product Name | Application | Type |
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| NS-030CN-F(E) | Mouse Anti-GD2 Recombinant Antibody (clone 3G6); Fab Fragment | WB | Mouse Fab |
| CAT | Product Name | Application | Type |
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| NS-029CN-S(P) | Mouse Anti-GD2 Recombinant Antibody (clone 5F11); scFv Fragment | WB | Mouse scFv |
| CAT | Product Name | Application | Type |
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| NS-030CN-S(P) | Mouse Anti-GD2 Recombinant Antibody (clone 3G6); scFv Fragment | WB | Mouse scFv |
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