Mouse Anti-GD2 Recombinant Antibody (clone 3G6) (CAT#: NS-030CN)
A recombinant antibody construct comprises the variable regions of the heavy and light chain of an anti-GD2 antibody.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human GD2
- Species Reactivity
- Human
- Clone
- 3G6
- Applications
- WB
- Related Disease
- Tumor
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Analysis of cell surface GD2, complement inhibitor expression, and C3 deposition was done as described. For analysis of mouse C3 deposition on EL4 cells in vitro, cells (5 × 10⁵) were resuspended in 20 mL PBS or anti-GD2 mAb (either 14G2a or 3G6) at 50 mg/ml and incubated for 30 minutes at 4°C. After washing, cells were resuspended in 20 mL PBS or complement inhibitor blocking mAb (5D5 and/or 3B3; final concentration, 50 mg/ml) and incubated at 4°C for 30 minutes. Cells were then washed and incubated in 30 mL of 30% mouse serum diluted in gelatin veronal-buffered saline (GVB++; Sigma) at 37°C for 30 minutes. Cells were then washed with EDTA-GVB, incubated with FITC-conjugated goat anti-mouse C3 (30 minutes/4°C), and washed twice. Finally, cells were suspended in PBS containing 2 mg/ml propidium iodide and analyzed by flow cytometry. Propidium iodide–positive (dead) cells were excluded when fluorescence intensity was calculated.
Target
- Alternative Names
- GD2 ganglioside; GD2
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-770-sdAb | Recombinant Anti-Human GD2 VHH Single Domain Antibody | Neut, ChiP, IP, FUNC | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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