Mouse Anti-GD2 Recombinant Antibody (clone 7A4) (CAT#: FAMAB-0126YC)
A antibody directed against GD2 (7A4) was cloned, sequenced, and expressed. Its binding properties were characterized and compared to that of the parental mab 7A4. The 7A4 bound to GD2+ neuroblastoma cell lines but not to GD2- cell lines or to GD2- cells isolated from peripheral blood. ELISA and thin-layer chromatography (TLC) indicated that it retained the anti-GD2 specificity, and exhibited a slight cross-reaction with GD3 as the parental mab. This antibody makes it possible to develop new useful reagents through genetic engineering for adjuvant tumor therapy.
Specific Inquiry
FC
Figure 1 Binding of the MAb 7A4.
Gd2+ LAN 1 neuroblastoma cells were incubated with either the parental Mab 74A (upper left panel) or the scFv 7A4 (lower left panel).
WB
Figure 2 Immunostaining of purified gangliosides on HPTLC plates by the scFv 7A4
After chromatography, purified gangliosides were immunostained with the Mab 7A4 or with the scFv 7A4.
ELISA
Figure 3 ELISA binding of scFv 7A4 to purified gangliosides.
Binding of various concentrations of MAb 7A4 or scFv 7A4 to purified GD2.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG3, κ
- Specificity
- Human GD2
- Species Reactivity
- Human
- Clone
- 7A4
- Applications
- ELISA
- Related Disease
- Disseminated neuroblastoma
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- GD2; Disialoganglioside
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-770-sdAb | Recombinant Anti-Human GD2 VHH Single Domain Antibody | Neut, ChiP, IP, FUNC | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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