Recombinant Anti-human HTT Intrabody [(D-Arg)₉]

Name: Recombinant Anti-human HTT Intrabody [(D-Arg)9]
SKU: IAB-B023(A)
Rating: 5 (1 reviews)

CAT#: IAB-B023(A)

Recombinant anti-HTT intrabody linked to (D-Arg)₉ works within cells and directs against htt and prevent aggregation, show promise as immunotherapeutics for HD, expressed in 293T cells. The anti-huntingtin intrabody can degrade mutant huntingtin proteins by directing them to the proteasome, while also increasing the negative charge.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/if_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human cerebral cortex

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/55354_B_9_5_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/299_A6_2_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/298_A6_1_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_B_8_5.jpg

Normal Tissue Figure 6 Colon

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_7_3.jpg

Normal Tissue Figure 7 Kidney

Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_9_5.jpg

Normal Tissue Figure 8 Testis

Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_4_6.jpg

Normal Tissue Figure 9 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_8_8.jpg

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Specifications

Immunogen
human Huntingtin

Host Species
human

Type
scFv-(D-Arg)₉

Cell Penetrating Peptide
(D-Arg)₉
It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.

Species Reactivity
human

Applications
Products can be used for: Enzyme-linked Immunosorbent Assay; Western blot; Immunocytochemistry; Functional studies

Product Property

Purity
>90% assessed by SDS-PAGE

Storage
Store at -20°C. Avoid multiple freeze/thaw cycles.

Target

Alternative Names
HTT; huntingtin; HD; IT15; huntington disease protein

Gene ID
3064

UniProt ID
P42858

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - IAB-B023(A).pdf

MSDS

MSDS - IAB-B023(A).pdf

COA

Certificate of Analysis Lookup

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