Anti-HTT Immunohistochemistry Kit
CAT#: VS-0325-XY1080
Our HTT IHC kit comes with a comprehensive set of reagents. The operation protocol is straightforward, allowing researchers to quickly conduct experiments. The high - affinity antibodies ensure clear and consistent staining of HTT, facilitating in - depth research on HTT - related neurodegenerative mechanisms. This kit can be applied to paraffin sections and frozen sections.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/55354_B_9_5_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/299_A6_2_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/298_A6_1_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_4_6.jpg
Normal Tissue
Figure 9 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_8_8.jpg
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat
- Target
- HTT
- Primary Antibody
- Rabbit Anti-HTT Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Datasheet
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COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| MHH-521 | Recombinant Human Anti-human HTT Antibody | WB, Neut, FuncS | IgG |
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| PABL-581 | Human Anti-HTT Recombinant Antibody (clone E51) | FC, ELISA | Human IgG |
| HPAB-0123-YC | Mouse Anti-HTT Recombinant Antibody (HPAB-0123-YC) | IHC, WB, Inhib | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-521-F(E) | Recombinant Anti-human HTT Antibody Fab Fragment | IP, IF, FuncS | Fab |
| MHH-521-F(E) | Recombinant Human Anti-human HTT Antibody Fab Fragment | Neut, WB, FuncS | Fab |
| PFBL-575 | Human Anti-HTT Recombinant Antibody (clone C4); Fab Fragment | Block | Human Fab |
| PFBL-576 | Human Anti-HTT Recombinant Antibody (clone E51); Fab Fragment | FC, ELISA | Human Fab |
| HPAB-0121-YC-F(E) | Mouse Anti-HTT Recombinant Antibody (clone MW1); Fab Fragment | ELISA, FC, WB, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-521-S(P) | Recombinant Anti-human HTT Antibody scFv Fragment | FC, Neut, Funcs | scFv |
| MHH-521-S(P) | Recombinant Human Anti-human HTT Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| PSBL-575 | Human Anti-HTT Recombinant Antibody (clone C4); scFv Fragment | Block | Human scFv |
| PSBL-576 | Human Anti-HTT Recombinant Antibody (clone E51); scFv Fragment | FC, ELISA | Human scFv |
| HPAB-0121-YC-S(P) | Mouse Anti-HTT Recombinant Antibody (clone MW1); scFv Fragment | ELISA, FC, WB, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B023(A) | Recombinant Anti-human HTT Intrabody [(D-Arg)9] | ELISA, WB, ICC, FuncS | scFv-(D-Arg)9 |
| IAB-B023(G) | Recombinant Anti-human HTT Intrabody [+36 GFP] | IF, FC, WB, FuncS | scFv-(+36GFP) |
| IAB-B023(T) | Recombinant Anti-human HTT Intrabody [Tat] | WB, CO-IP, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
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| TAB-114CT | Human Anti-HTT Recombinant Antibody (TAB-114CT) | ELISA, WB | Human IgG1, κ |
| TAB-114CT-S(P) | Human Anti-HTT Recombinant Antibody; scFv Fragment (TAB-114CT-S(P)) | ELISA, WB | Human scFv |
| TAB-114CT-F(E) | Human Anti-HTT Recombinant Antibody; Fab Fragment (TAB-114CT-F(E)) | ELISA, WB | Human Fab |
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| CAT | Product Name | Application | Type |
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| TAB-115CT-S(P) | Mouse Anti-HTT Recombinant Antibody; scFv Fragment (TAB-115CT-S(P)) | ELISA, WB | Mouse scFv |
| TAB-116CT-S(P) | Mouse Anti-HTT Recombinant Antibody; scFv Fragment (TAB-116CT-S(P)) | ELISA, WB | Mouse scFv |
| TAB-115CT-F(E) | Mouse Anti-HTT Recombinant Antibody; Fab Fragment (TAB-115CT-F(E)) | ELISA, WB | Mouse Fab |
| TAB-116CT-F(E) | Mouse Anti-HTT Recombinant Antibody; Fab Fragment (TAB-116CT-F(E)) | ELISA, WB | Mouse Fab |
| TAB-117CT-F(E) | Mouse Anti-HTT Recombinant Antibody; Fab Fragment (TAB-117CT-F(E)) | ELISA, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1714 | Rabbit Anti-HTT Recombinant Antibody (clone DS1714AB) | WB, IHC-P, ICC | Rabbit IgG |
| MOR-0066-FY | Rabbit Anti-HTT Recombinant Antibody (clone AFY0037) | FC, ICC, IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0122-YC | Mouse Anti-HTT Recombinant Antibody (HPAB-0122-YC) | FuncS, WB, Inhib, IHC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-039CN | Human Anti-HTT Recombinant Antibody (NS-039CN) | ELISA, DB, IHC, FuncS | Human IgG |
| NS-039CN-F(E) | Human Anti-HTT Recombinant Antibody; Fab Fragment (NS-039CN-F(E)) | ELISA | Human Fab |
| NS-039CN-S(P) | Human Anti-HTT Recombinant Antibody; scFv Fragment (NS-039CN-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
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| VS-0425-YC384 | Recombinant Anti-HTT Vesicular Antibody, EV Displayed (VS-0425-YC384) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
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| VS-0525-XY3360 | Anti-Mouse HTT Immunohistochemistry Kit | IHC | |
| VS-0525-XY3359 | Anti-Human HTT Immunohistochemistry Kit | IHC |
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