Anti-HTT Intrabody [Tat] (IAB-B023(T))
Recombinant anti-HTT intrabody in fusion with HIV Tat works within cells and is directed against htt and prevent aggregation, show promise as immunotherapeutics for HD, expressed in 293T cells. The anti-huntingtin intrabody can degrade mutant huntingtin proteins by directing them to the proteasome, while also increasing the negative charge.
Specifications
- Immunogen
- human Huntingtin
- Host
- human
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Formats of immunological tests: Western blot; Coimmunopricipation; Functional studies
Target
- Alternative Names
- HTT; huntingtin; HD; IT15; huntington disease protein
- Entrez Gene ID
- 3064
- UniProt ID
- P42858
For lab research use only, not for diagnostic, therapeutic or any in vivo human use.
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