Recombinant Anti-human HTT Intrabody [Tat]

Name: Recombinant Anti-human HTT Intrabody [Tat]
SKU: IAB-B023(T)
Rating: 5 (1 reviews)

CAT#: IAB-B023(T)

Recombinant anti-HTT intrabody in fusion with HIV Tat works within cells and is directed against htt and prevent aggregation, show promise as immunotherapeutics for HD, expressed in 293T cells. The anti-huntingtin intrabody can degrade mutant huntingtin proteins by directing them to the proteasome, while also increasing the negative charge.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/if_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human cerebral cortex

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/55354_B_9_5_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/299_A6_2_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/298_A6_1_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_B_8_5.jpg

Normal Tissue Figure 6 Colon

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_7_3.jpg

Normal Tissue Figure 7 Kidney

Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_9_5.jpg

Normal Tissue Figure 8 Testis

Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_4_6.jpg

Normal Tissue Figure 9 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_8_8.jpg

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Specifications

Immunogen
human Huntingtin

Host Species
human

Type
scFv-Tat

Cell Penetrating Peptide
TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.

Species Reactivity
human

Applications
Formats of immunological tests: Western blot; Coimmunopricipation; Functional studies

Product Property

Purity
>95% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE

Storage
Store at -20°C. Open under aseptic conditions.

Target

Alternative Names
HTT; huntingtin; HD; IT15; huntington disease protein

Gene ID
3064

UniProt ID
P42858

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - IAB-B023(T).pdf

MSDS

MSDS - IAB-B023(T).pdf

COA

Certificate of Analysis Lookup

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