Recombinant Anti-human HTT Intrabody [Tat] (CAT#: IAB-B023(T))
Recombinant anti-HTT intrabody in fusion with HIV Tat works within cells and is directed against htt and prevent aggregation, show promise as immunotherapeutics for HD, expressed in 293T cells. The anti-huntingtin intrabody can degrade mutant huntingtin proteins by directing them to the proteasome, while also increasing the negative charge.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/if_selected.jpg
Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/55354_B_9_5_selected.jpg
(Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/55354_B_9_5_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/299_A6_2_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/299_A6_2_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/298_A6_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26114/298_A6_1_red_green.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_B_8_5.jpg
(Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_B_8_5.jpg
Figure 6 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_7_3.jpg
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_7_3.jpg
Figure 7 Kidney
(Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_9_5.jpg
(Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_9_5.jpg
Figure 8 Testis
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_4_6.jpg
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_4_6.jpg
Figure 9 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_8_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2756/7010_A_8_8.jpg
Specifications
- Immunogen
- human Huntingtin
- Host Species
- human
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Formats of immunological tests: Western blot; Coimmunopricipation; Functional studies
Product Property
- Purity
- >95% as determined by Analysis by RP-HPLC & analysis by SDS-PAGE
- Storage
- Store at -20°C. Open under aseptic conditions.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "HTT"
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1905-VHH | Recombinant Anti-human HTT VHH Single Domain Antibody | IHC, ICC, FC, FA, FUNC | Llama VHH |
| PABJ-0201 | Human Anti-HTT Recombinant Antibody (clone 3LRH) | ELISA, FuncS | Human VL |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-521 | Recombinant Anti-human HTT Antibody | WB, Neut, FuncS | IgG |
| HPAB-0122-YC | Mouse Anti-HTT Recombinant Antibody (HPAB-0122-YC) | FuncS, WB, Inhib, IHC | Mouse IgM |
| HPAB-0123-YC | Mouse Anti-HTT Recombinant Antibody (HPAB-0123-YC) | IHC, WB, Inhib | Mouse IgG2a |
| HPAB-0125-YC | Human Anti-HTT Recombinant Antibody (HPAB-0125-YC) | ELISA, IHC | Humanized IgG |
| HPAB-0126-YC | Human Anti-HTT Recombinant Antibody (HPAB-0126-YC) | ELISA, IHC | Humanized IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-521-S(P) | Recombinant Anti-human HTT Antibody scFv Fragment | FC, Neut, Funcs | scFv |
| MHH-521-S(P) | Recombinant Human Anti-human HTT Antibody scFv Fragment | ELISA, WB, FuncS | scFv |
| PSBL-575 | Human Anti-HTT Recombinant Antibody (clone C4); scFv Fragment | Block | Human scFv |
| PSBL-576 | Human Anti-HTT Recombinant Antibody (clone E51); scFv Fragment | FC, ELISA | Human scFv |
| HPAB-0121-YC-S(P) | Mouse Anti-HTT Recombinant Antibody (clone MW1); scFv Fragment | ELISA, FC, WB, IHC | Mouse scFv |
Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B023(A) | Recombinant Anti-human HTT Intrabody [(D-Arg)9] | ELISA, WB, ICC, FuncS | scFv-(D-Arg)9 |
| IAB-B023(G) | Recombinant Anti-human HTT Intrabody [+36 GFP] | IF, FC, WB, FuncS | scFv-(+36GFP) |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-114CT | Human Anti-HTT Recombinant Antibody (TAB-114CT) | ELISA, WB | Human IgG1, κ |
| TAB-114CT-S(P) | Human Anti-HTT Recombinant Antibody; scFv Fragment (TAB-114CT-S(P)) | ELISA, WB | Human scFv |
| TAB-114CT-F(E) | Human Anti-HTT Recombinant Antibody; Fab Fragment (TAB-114CT-F(E)) | ELISA, WB | Human Fab |
| PABX-109 | Recombinant Human Anti-Huntingtin (Htt) Antibody | WB, Neut, FuncS | IgG |
| PABX-109-F (E) | Recombinant Human Anti-Huntingtin(Htt) Antibody Fab Fragment | WB, Neut, FuncS | Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-115CT-S(P) | Mouse Anti-HTT Recombinant Antibody; scFv Fragment (TAB-115CT-S(P)) | ELISA, WB | Mouse scFv |
| TAB-116CT-S(P) | Mouse Anti-HTT Recombinant Antibody; scFv Fragment (TAB-116CT-S(P)) | ELISA, WB | Mouse scFv |
| TAB-117CT-S(P) | Mouse Anti-HTT Recombinant Antibody; scFv Fragment (TAB-117CT-S(P)) | ELISA, WB | Mouse scFv |
| TAB-115CT-F(E) | Mouse Anti-HTT Recombinant Antibody; Fab Fragment (TAB-115CT-F(E)) | ELISA, WB | Mouse Fab |
| PABX-109-S (P) | Recombinant Human Anti-Huntingtin (Htt) Antibody scFv Fragment | WB, Neut, FuncS | scFv |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1714 | Rabbit Anti-HTT Recombinant Antibody (clone DS1714AB) | WB, IHC-P, ICC | Rabbit IgG |
| MOR-0066-FY | Rabbit Anti-HTT Recombinant Antibody (clone AFY0037) | FC, ICC, IHC, WB | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0121-YC-F(E) | Mouse Anti-HTT Recombinant Antibody (clone MW1); Fab Fragment | ELISA, FC, WB, IHC | Mouse Fab |
| HPAB-0122-YC-F(E) | Mouse Anti-HTT Recombinant Antibody; Fab Fragment (HPAB-0122-YC-F(E)) | ELISA, FC, WB, IHC | Mouse Fab |
| HPAB-0123-YC-F(E) | Mouse Anti-HTT Recombinant Antibody; Fab Fragment (HPAB-0123-YC-F(E)) | ELISA, FC, WB, IHC | Mouse Fab |
| HPAB-0125-YC-F(E) | Human Anti-HTT Recombinant Antibody; Fab Fragment (HPAB-0125-YC-F(E)) | ELISA, IHC | Humanized Fab |
| HPAB-0126-YC-F(E) | Human Anti-HTT Recombinant Antibody; Fab Fragment (HPAB-0126-YC-F(E)) | ELISA, IHC | Humanized Fab |
Neuroscience Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-039CN | Human Anti-HTT Recombinant Antibody (NS-039CN) | ELISA, DB, IHC, FuncS | Human IgG |
| NS-039CN-F(E) | Human Anti-HTT Recombinant Antibody; Fab Fragment (NS-039CN-F(E)) | ELISA | Human Fab |
| NS-039CN-S(P) | Human Anti-HTT Recombinant Antibody; scFv Fragment (NS-039CN-S(P)) | ELISA | Human scFv |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for IAB-B023(T). Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.