How can this RTN-4 antibody be used across multiple applications?

We have validated the Anti-RTN-4 Recombinant Antibody in several applications including ELISA, flow cytometry, immunoprecipitation, and immunofluorescence. Its high purity and detailed performance data, such as>97% purity under reducing conditions, make it an ideal reagent for various in vitro studies focused on reticulon 4 detection.

What handling instructions are recommended for this antibody?

We advise that users strictly follow the storage and handling guidelines, including storing under sterile conditions at -20°C and avoiding repeated freeze-thaw cycles. The comprehensive product details provide clear directions on dilution and incubation parameters, which contribute to consistent performance in immunoassays for reticulon 4.

Anti-Human RTN-4 Recombinant Antibody (TAB-242)

CAT#: TAB-242

Recombinant monoclonal antibody to RTN-4. It is an immunomodulator. It binds to reticulon 4.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23977/if_selected.jpg

Normal Tissue Figure 2 IHC staining of human testis

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in seminiferous ducts cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23977/54863_A_4_6_selected.jpg

Normal Tissue Figure 3 IHC staining of human testis

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5388/13996_A_6_6_selected.jpg

Normal Tissue Figure 4 Cerebral cortex

Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23977/54863_B_8_5.jpg

Normal Tissue Figure 5 Cerebellum

Cells in granular layer Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Cells in molecular layer Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5388/13996_B_7_8.jpg

Normal Tissue Figure 6 Colon

Enterocytes - Mucosal lymphoid cells Staining: Low Intensity: Moderate Quantity: <25% Peripheral nerve/ganglion Staining: High Intensity: Strong

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23977/54863_A_9_3.jpg

Normal Tissue Figure 7 Testis

Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/23977/54863_A_4_6.jpg

RNA Expression Figure 8 RNA cell line category: Cell line enhanced (HHSteC)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115310-RTN4

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Specifications

Immunogen
The details of the immunogen for this antibody are not available.

Host Species
Human

Derivation
Human

Type
IgG4 - kappa

Specificity
Tested positive against native human antigen.

Species Reactivity
Human

Applications
Suitable for use in WB, Neut, ELISA, IF, IP, FuncS, FC and most other immunological methods.

Related Disease
Spinal cord injury, acute

Product Property

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Storage
Store it under sterile conditions at -20°C upon receiving. Recommend to pack the protein into smaller quantities for optimal storage.

Target

Alternative Names
RTN4; reticulon 4; reticulon-4; ASY; KIAA0886; NOGO; NSP CL; foocen; Human NogoA; reticulon 5; My043 protein; neurite outgrowth inhibitor; neurite growth inhibitor 220; neuroendocrine-specific protein C homolog; NSP; NOGOC; RTN-X; NOGO-A; NSP-C

Gene ID
57142

UniProt ID
Q9NQC3

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Customer Review

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Breast cancer biomarkers at key points during disease progression

Multifunctional Tool

The Anti-RTN-4 Recombinant Antibody has been extremely useful in my research work. I have used it in ELISA, Immunofluorescence, and Immunoprecipitation with remarkable consistency. The rich product details regarding its purity (>97% under reducing conditions) and extensive validation across numerous immunoassays helped in optimizing my experimental protocols.

Excellent Versatility

I found the RTN-4 antibody to be a versatile reagent, performing well in flow cytometry and functional assays. The detailed protocol recommendations and high purity information provided clear guidance for experimental use. The antibody's performance in multiple assay formats has been consistently reliable for studying reticulon 4 in various research settings.

Q&As

How can this RTN-4 antibody be used across multiple applications?

A: We have validated the Anti-RTN-4 Recombinant Antibody in several applications including ELISA, flow cytometry, immunoprecipitation, and immunofluorescence. Its high purity and detailed performance data, such as>97% purity under reducing conditions, make it an ideal reagent for various in vitro studies focused on reticulon 4 detection.
What handling instructions are recommended for this antibody?

A: We advise that users strictly follow the storage and handling guidelines, including storing under sterile conditions at -20°C and avoiding repeated freeze-thaw cycles. The comprehensive product details provide clear directions on dilution and incubation parameters, which contribute to consistent performance in immunoassays for reticulon 4.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-242.pdf

MSDS

MSDS - TAB-242.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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