Rat Anti-IL18 Recombinant Antibody (clone 2C10); Fab Fragment (CAT#: HPAB-0067-YC-F(E))

Figure 1 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC-F(E)) in DB

Dot Blot analysis of HPAB-0067-YC-F(E) was performed by coating with human IL18 protein (His tag).
HPAB-0067-YC-F(E) incubation concentration: 2 μg/mL.
HRP conjugated Anti-Rat IgG (H+L) as a secondary antibody (1: 3000)

Figure 2 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC-F(E)) in WB

Western blot analysis of HPAB-0067-YC-F(E) was performed by loading human IL18 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0067-YC-F(E) and HRP conjugated Anti-Rat IgG (H+L) as a secondary antibody (1: 3000). Chemiluminescent detection was performed.

Lane1: Reduced antigen (0.1 μg)
Lane2: Reduced antigen (0.3 μg)
Lane3: Reduced antigen (0.6 μg)

Figure 3 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC-F(E)) in ELISA

ELISA analysis of HPAB-0067-YC-F(E) was performed by coating with human IL18 protein (His tag). Then blocked with BSA and incubated with HPAB-0067-YC-F(E). The HRP conjugated Anti-His tag as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.