Rat Anti-IL18 Recombinant Antibody (clone 2C10); Fab Fragment (CAT#: HPAB-0067-YC-F(E))
Interleukin-18 (IL-18) is a potent cytokine that plays a role in both innate and acquired immune responses. IL-18 was first identified for its ability to induce the production of IFN-ɣ, one of the primary effector molecules of a T helper (Th) 1-type immune response. Anti-IL-18 antibodies may be useful for example, in diagnosing and treating disorders associated with elevated IL-18 levels, including inflammatory, cardiovascular and autoimmune diseases
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DB
-1.jpg)
Figure 1 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC-F(E)) in DB
Dot Blot analysis of HPAB-0067-YC-F(E) was performed by coating with human IL18 protein (His tag).
HPAB-0067-YC-F(E) incubation concentration: 2 μg/mL.
HRP conjugated Anti-Rat IgG (H+L) as a secondary antibody (1: 3000)
WB
-2.jpg)
Figure 2 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC-F(E)) in WB
Western blot analysis of HPAB-0067-YC-F(E) was performed by loading human IL18 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0067-YC-F(E) and HRP conjugated Anti-Rat IgG (H+L) as a secondary antibody (1: 3000). Chemiluminescent detection was performed.
Lane1: Reduced antigen (0.1 μg)
Lane2: Reduced antigen (0.3 μg)
Lane3: Reduced antigen (0.6 μg)
ELISA
-3.jpg)
Figure 3 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC-F(E)) in ELISA
ELISA analysis of HPAB-0067-YC-F(E) was performed by coating with human IL18 protein (His tag). Then blocked with BSA and incubated with HPAB-0067-YC-F(E). The HRP conjugated Anti-His tag as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- Recombinant IL-18
- Host Species
- Rat
- Type
- Rat Fab
- Specificity
- Human IL18
- Species Reactivity
- Human
- Clone
- 2C10
- Applications
- Neut
- Related Disease
- Inflammatory, Cardiovascular, Autoimmune diseases
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Related Resources
Inflammatory Bowel Diseases
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Biparatopic Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-607 | Human Anti-IL18 Recombinant Antibody (clone YC126) | WB, ELISA, FuncS | Human IgG |
| HPAB-0064-YC | Human Anti-IL18 Recombinant Antibody (clone 2E1) | ELISA, RIA, IHC, FuncS, Neut | Human IgG |
| HPAB-0649-CN | Human Anti-IL18 Recombinant Antibody (HPAB-0649-CN) | ELISA, Neut | Humanized IgG1, κ |
| FAMAB-0041JF | Human Anti-IL18 Recombinant Antibody (clone h18-108) | WB, IF, IHC, ELISA | Human IgG |
| VS3-FY748 | Recombinant Rabbit Anti-IL18 Antibody (clone R02-8B2) | WB, IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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