Rat Anti-IL18 Recombinant Antibody (clone 2C10) (CAT#: HPAB-0067-YC)
Interleukin-18 (IL-18) is a potent cytokine that plays a role in both innate and acquired immune responses. IL-18 was first identified for its ability to induce the production of IFN-ɣ, one of the primary effector molecules of a T helper (Th) 1-type immune response. Anti-IL-18 antibodies may be useful for example, in diagnosing and treating disorders associated with elevated IL-18 levels, including inflammatory, cardiovascular and autoimmune diseases
Specific Inquiry
DB
Figure 1 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC) in DB
Dot Blot analysis of HPAB-0067-YC was performed by coating with human IL18 protein (His tag).
HPAB-0067-YC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Rat IgG as a secondary antibody (1: 2000)
WB
Figure 2 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC) in WB
Western blot analysis of HPAB-0067-YC was performed by loading human IL18 protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0067-YC and HRP goat anti-Rat IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
ELISA
Figure 3 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC) in ELISA
ELISA analysis of HPAB-0067-YC was performed by coating with human IL18 protein (His tag). Then blocked with BSA and incubated with HPAB-0067-YC. The HRP-conjugated goat anti-Rat IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- Recombinant IL-18
- Host Species
- Rat
- Type
- Rat IgG
- Specificity
- Human IL18
- Species Reactivity
- Human
- Clone
- 2C10
- Applications
- Neut
- Related Disease
- Inflammatory, Cardiovascular, Autoimmune diseases
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Related Resources
Inflammatory Bowel Diseases
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBW-081 | Mouse Anti-IL18 Recombinant Antibody (clone 125-2H); Fab Fragment | ELISA, Neut | Mouse Fab |
| HPAB-1273LY-F(E) | Human Anti-IL18 Recombinant Antibody; Fab Fragment (HPAB-1273LY-F(E)) | ELISA, Inhib | Human Fab |
| HPAB-1274LY-F(E) | Human Anti-IL18 Recombinant Antibody; Fab Fragment (HPAB-1274LY-F(E)) | ELISA, Inhib | Human Fab |
| HPAB-1275LY-F(E) | Human Anti-IL18 Recombinant Antibody; Fab Fragment (HPAB-1275LY-F(E)) | ELISA, Inhib | Human Fab |
| HPAB-1276LY-F(E) | Human Anti-IL18 Recombinant Antibody; Fab Fragment (HPAB-1276LY-F(E)) | ELISA, Inhib | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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