Rat Anti-IL18 Recombinant Antibody (clone 2C10) (CAT#: HPAB-0067-YC)

Figure 1 show representative data from one experiment that generated the EC50 values.

Figure 2 show representative data from one experiment that generated the EC50 values.

Figure 3 show representative data from one experiment that generated the EC50 values.

Figure 4 shows 2C10 binding to hIL-18 captured by hiL 18-BP

Demonstrates that 2C10 is bound to hIL-18 that, in turn, had been captured by hIL-18BP, indicating that the binding sites for 2C10 and hIL-18BP are not Overlapping.

Figure 5 illustrates a representative experiment.

shows inhibition of IL-18 stimulated IFN-Y pro duction by the H1 variants and 2C10 in KG1 cells.

Figure 1 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC) in DB

Dot Blot analysis of HPAB-0067-YC was performed by coating with human IL18 protein (His tag).
HPAB-0067-YC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Rat IgG as a secondary antibody (1: 2000)

Figure 2 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC) in WB

Western blot analysis of HPAB-0067-YC was performed by loading human IL18 protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0067-YC and HRP goat anti-Rat IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Figure 3 Rat Anti-IL18 Recombinant Antibody (HPAB-0067-YC) in ELISA

ELISA analysis of HPAB-0067-YC was performed by coating with human IL18 protein (His tag). Then blocked with BSA and incubated with HPAB-0067-YC. The HRP-conjugated goat anti-Rat IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.