Anti-Human LAG3 Recombinant Antibody (TSR-033)
CAT#: TAB-0367CL
This LAG3-specific antibody demonstrates a potent and durable anti-tumor activity in syngeneic tumor models. It can be used for immunotherapy.
Published Data
Tested Data
Gene Expression
FC
Figure 1 TSR-033 binds with high affinity to human and cyno LAG-3.
TSR-033 binds human and cyno LAG-3 expressed on the surface of CHO-S cells, but not mouse LAG-3, as assessed and flow cytometry.
Ghosh, S., Sharma, G., Travers, J., Kumar, S., Choi, J., Jun, H. T., ... & Jenkins, D. (2019). TSR-033, a Novel Therapeutic Antibody Targeting LAG-3, Enhances T-Cell Function and the Activity of PD-1 Blockade In Vitro and In Vivo. Molecular cancer therapeutics, 18(3), 632-641.
FuncS
Figure 2 TSR-033 amplifies T cell effector function, particularly in combination with anti-PD-1.
The functional activity of TSR-033 was evaluated in an MLR assay, in which primary human CD4 T cells were mixed with monocyte-derived dendritic cells from a different donor. The ability of TSR-033 and TSR-042 to induce IL-2 from human PBMCs (n=5 donors) stimulated with 100 ng/mL SEB for 3 days was assessed. Data are representative of at least two separate occasions.
Ghosh, S., Sharma, G., Travers, J., Kumar, S., Choi, J., Jun, H. T., ... & Jenkins, D. (2019). TSR-033, a Novel Therapeutic Antibody Targeting LAG-3, Enhances T-Cell Function and the Activity of PD-1 Blockade In Vitro and In Vivo. Molecular cancer therapeutics, 18(3), 632-641.
Block
Figure 3 Dual blockade of LAG-3 and PD-1 using TSR-033 and TSR-042 improves therapeutic efficacy and T cell stimulation in a humanized NSCLC tumor mouse model.
The combination of TSR-033 with TSR-042 has an additive effect (CDI=1.001) on restricting tumor growth in HuNOG-EXL mice inoculated with A549 cells. Mice were randomized at tumor volumes of 80-120 mm³, followed by administration of the indicated regimens (Materials and methods). Tumor growth inhibition at termination for each treatment arm is indicated in parentheses.
Ghosh, S., Sharma, G., Travers, J., Kumar, S., Choi, J., Jun, H. T., ... & Jenkins, D. (2019). TSR-033, a Novel Therapeutic Antibody Targeting LAG-3, Enhances T-Cell Function and the Activity of PD-1 Blockade In Vitro and In Vivo. Molecular cancer therapeutics, 18(3), 632-641.
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WB
Figure 1 Anti-Human LAG3 Recombinant Antibody (TAB-0367CL) in WB
Western blot analysis of TAB-0367CL was performed with Human LAG3 Protein (His Tag) in reduced condition Lane 1, Lane 2, Lane 3 (0.1 μg, 0.3 μg, 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-0367CL (2 ng/μL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
ELISA
Figure 2 Anti-Human LAG3 Recombinant Antibody (TAB-0367CL) in ELISA
ELISA analysis of TAB-0367CL was performed by coating with Human LAG3 Protein (His Tag). Then blocked with BSA and incubated with TAB-0367CL. The HRP-conjugated goat anti-human IgG was used as a secondary antibody (1:2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Normal Tissue
Figure 1 IHC staining of human tonsil
High expression. RNA expression: 10.9 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13967/30401_A_4_8_val_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebral cortex
Low expression. RNA expression: 0.7 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13967/30401_B_9_5_val_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13967/30401_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2301/5962_A_7_4.jpg
Normal Tissue
Figure 5 Testis
Leydig cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2301/5962_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13967/30401_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enriched (HDLM-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000089692-LAG3
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Specifications
- Immunogen
- Human LAG-3 ECD (for panning)
- Host Species
- Human
- Type
- Human antibody
- Antibody Isotype
- IgG
- Specificity
- Human
- Clone
- TSR-033
- Applications
- FC, FuncS, Block
- Related Disease
- Tumor
Applications
- Application Notes
- The LAG-3 antibody has been reported in applications of Flow Cytometry, Functional Assay, Blocking.
Target
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(Creative Biolabs Cat# TAB-0367CL, RRID: AB_3111772)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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