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    Hi-Affi™ Recombinant Rabbit Anti-MDM2 Monoclonal Antibody (TH155DS) (CAT#: MOR-4642)

    Recombinant Rabbit anti-MDM2 monoclonal antibody for WB, IF, ICC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/16303/if_selected.jpg
    Figure 2 IF staining of human cell line U-2 OS
    Figure 2 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/79977/1999_F7_3_selected.jpg
    Figure 3 IHC staining of human cerebellum
    Figure 3 IHC staining of human cerebellum
    (Immunohistochemical staining of human cerebellum shows strong nuclear positivity.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/ihc_selected.jpg
    Figure 4 IHC staining of human ovary
    Figure 4 IHC staining of human ovary
    (Immunohistochemical staining of human ovary shows strong nuclear positivity in ovarian stroma cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/16303/37938_A_6_7_selected.jpg
    Figure 5 IHC staining of human placenta
    Figure 5 IHC staining of human placenta
    (Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/79977/170698_A_2_7_selected.jpg
    Normal Tissue
    Figure 6 Cerebral cortex
    Figure 6 Cerebral cortex
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Glial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Neuronal cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_B_8_5.jpg
    Figure 7 Colon
    Figure 7 Colon
    (Endothelial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Glandular cells
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear
    Peripheral nerve/ganglion
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_9_3.jpg
    Figure 8 Liver
    Figure 8 Liver
    (Cholangiocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Hepatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_8_4.jpg
    Figure 9 Pancreas
    Figure 9 Pancreas
    (Exocrine glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Pancreatic endocrine cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_2_3.jpg
    Figure 10 Kidney
    Figure 10 Kidney
    (Cells in glomeruli
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_8_5.jpg
    Figure 11 Testis
    Figure 11 Testis
    (Pachytene spermatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Peritubular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Preleptotene spermatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Round or early spermatids
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Spermatogonia cells
    Staining: High
    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_5_6.jpg
    Figure 12 Lymph node
    Figure 12 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Non-germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_9_8.jpg
    RNA Expression
    Figure 13 RNA cell line category: Cell line enhanced (HBEC3-KT, U-698)
    Figure 13 RNA cell line category: Cell line enhanced (HBEC3-KT, U-698)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135679-MDM2

    Specifications

    • Immunogen
    • Recombinant protein corresponding to amino acids 215-422 of human MDM2.
    • Host Species
    • Rabbit
    • Type
    • IgG
    • Species Reactivity
    • Rat, Human, Mouse
    • Clone
    • TH155DS
    • Applications
    • Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunocytochemistry
    • Conjugate
    • Unconjugated

    Product Property

    • Purification
    • Purified with Absolute Mag™ Protein A Agarose
    • Purity
    • > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
    • Format
    • Liquid
    • Buffer
    • PBS
    • Preservative
    • 0.09% sodium azide
    • Storage
    • Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.

    Applications

    • Application Notes
    • ICC: 1-3 µg/ml; IF: 1-3 µg/ml; WB: 0.1-1 µg/ml

    Target

    • Alternative Names
    • MDM2 Proto-Oncogene; MDM2 Proto-Oncogene, E3 Ubiquitin Protein Ligase; Oncoprotein Mdm2; Hdm2; Mdm2, Transformed 3T3 Cell Double Minute 2, P53 Binding Protein (Mouse); Mdm2, Transformed 3T3 Cell Double Minute 2, P53 Binding Protein; Mouse Double Minute 2, Human Homolog Of; P53-Binding Protein; Double Minute 2, Human Homolog Of; P53-Binding Protein; Mdm2, P53 E3 Ubiquitin Protein Ligase Homolog; MDM2 Oncogene, E3 Ubiquitin Protein Ligase; Mdm2 P53 Binding Protein Homolog (Mouse); P53-Binding Protein Mdm2; Double Minute 2 Protein; EC 6.3.2.-; EC 6.3.2; ACTFS; HDMX

    Related Resources

    • Related Diseases
    • Related Signaling Pathways

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    VS3-XY1056 Mouse Anti-MDM2 Recombinant Antibody (clone 3G2) WB, IP, IF, IHC Mouse IgG1
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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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