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    Mouse Anti-MDM2 Recombinant Antibody (clone 3G2) (CAT#: VS3-XY1056)

    Clone 3G2 is a mouse antibody, which reacts with MDM2 protein. This antibody is suitable for MDM2 detection by WB, IP, IF, IHC assays.

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    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/16303/if_selected.jpg
    Figure 2 IF staining of human cell line U-2 OS
    Figure 2 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/79977/1999_F7_3_selected.jpg
    Figure 3 IHC staining of human cerebellum
    Figure 3 IHC staining of human cerebellum
    (Immunohistochemical staining of human cerebellum shows strong nuclear positivity.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/ihc_selected.jpg
    Figure 4 IHC staining of human ovary
    Figure 4 IHC staining of human ovary
    (Immunohistochemical staining of human ovary shows strong nuclear positivity in ovarian stroma cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/16303/37938_A_6_7_selected.jpg
    Figure 5 IHC staining of human placenta
    Figure 5 IHC staining of human placenta
    (Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/79977/170698_A_2_7_selected.jpg
    Normal Tissue
    Figure 6 Cerebral cortex
    Figure 6 Cerebral cortex
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Glial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Neuronal cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_B_8_5.jpg
    Figure 7 Colon
    Figure 7 Colon
    (Endothelial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Glandular cells
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear
    Peripheral nerve/ganglion
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_9_3.jpg
    Figure 8 Liver
    Figure 8 Liver
    (Cholangiocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Hepatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_8_4.jpg
    Figure 9 Pancreas
    Figure 9 Pancreas
    (Exocrine glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Pancreatic endocrine cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_2_3.jpg
    Figure 10 Kidney
    Figure 10 Kidney
    (Cells in glomeruli
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_8_5.jpg
    Figure 11 Testis
    Figure 11 Testis
    (Pachytene spermatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Peritubular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Preleptotene spermatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Round or early spermatids
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Spermatogonia cells
    Staining: High
    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_5_6.jpg
    Figure 12 Lymph node
    Figure 12 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Non-germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/86/33293_A_9_8.jpg
    RNA Expression
    Figure 13 RNA cell line category: Cell line enhanced (HBEC3-KT, U-698)
    Figure 13 RNA cell line category: Cell line enhanced (HBEC3-KT, U-698)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000135679-MDM2

    Specifications

    • Immunogen
    • Amino acids 154-167 of MDM2 of human origin.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • MDM2
    • Species Reactivity
    • Human
    • Clone
    • 3G2
    • Applications
    • Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

    Product Property

    • Purification
    • Protein A affinity purified
    • Format
    • Liquid
    • Buffer
    • PBS
    • Preservative
    • 0.05% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

    Target

    • Alternative Names
    • MDM2 Proto-Oncogene; MDM2 Proto-Oncogene, E3 Ubiquitin Protein Ligase; Oncoprotein Mdm2; Hdm2; Mdm2, Transformed 3T3 Cell Double Minute 2, P53 Binding Protein (Mouse); Mdm2, Transformed 3T3 Cell Double Minute 2, P53 Binding Protein; Mouse Double Minute 2, Human Homolog Of; P53-Binding Protein; Double Minute 2, Human Homolog Of; P53-Binding Protein; Mdm2, P53 E3 Ubiquitin Protein Ligase Homolog; MDM2 Oncogene, E3 Ubiquitin Protein Ligase

    Related Resources

    • Related Diseases
    • Related Signaling Pathways

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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