Anti-Human NOTCH3 Recombinant Antibody (TAB-559MZ)
CAT#: TAB-559MZ
This antibody has similar activity in blocking Notch2 and Notch 3 signaling. This Notch-binding agent works as an antagonist and for treating diseases such as cancer.
Tested Data
Gene Expression
FC
Figure 1 Anti-Human NOTCH3 Recombinant Antibody (TAB-559MZ) in FC
Flow cytometry analysis of U87 MG cells with purified antibody TAB-559MZ (QCAb253, Blue). Cells were blocked with Human TruStain FcXTM and washed with 0.5% BSA-PBS. Cells were then incubated with TAB-559MZ (5 μl/5x10^5 cells) for 60 min at 4°C. The secondary antibody was FITC anti-human IgG antibody (3 μl) for 60 min at 4°C.
SEC-HPLC
Figure 2 Anti-Human NOTCH3 Recombinant Antibody (TAB-559MZ) in SEC-HPLC
SEC-HPLC analysis of TAB-559MZ shows that the purity is more than 95%.
ELISA
Figure 3 Anti-Human NOTCH3 Recombinant Antibody (TAB-559MZ) in ELISA
ELISA analysis of TAB-559MZ was performed by coating with Recombinant Human NOTCH3 Protein.
The secondary antibody: HRP-anti-Human IgG
WB
Figure 4 Anti-Human NOTCH3 Recombinant Antibody (TAB-559MZ) in WB
Western blot analysis of TAB-559MZ was performed with Recombinant Human NOTCH3 Protein.
TAB-559MZ incubation concentration: 2 ng/μL.
The secondary antibody: Goat Anti-Human IgG(H+L)-HRP
Lane 1: Reducing antigen (0.1μg)
Lane 2: Reducing antigen (0.3μg)
Lane 3: Reducing antigen (0.6μg)
DB
Figure 5 Anti-Human NOTCH3 Recombinant Antibody (TAB-559MZ) in DB
Dot Blot analysis of TAB-559MZ was performed by coating with Recombinant Human NOTCH3 Protein.
The secondary antibody: HRP-anti-Human IgG
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong nuclear and moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (AF22, MCF7, RT4, U-87 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000074181-NOTCH3
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Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human antibody
- Specificity
- Human
- Applications
- FC, Inhib, FuncS
- Related Disease
- Breast cancer, pancreatic cancer, melanoma, colon cancer
Applications
- Application Notes
- The antibody was validated for Flow Cytometry, Inhibition, Functional Assay. For details, refer to Published Data.
Target
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Related Diseases
Breast Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "NOTCH3"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-554MZ | Human Anti-NOTCH3 Recombinant Antibody (TAB-554MZ) | FuncS, Inhib, WB | Human IgG |
| TAB-554MZ-S(P) | Human Anti-NOTCH3 Recombinant Antibody; scFv Fragment (TAB-554MZ-S(P)) | ELISA | Human scFv |
| TAB-555MZ-S(P) | Human Anti-NOTCH3 Recombinant Antibody; scFv Fragment (TAB-555MZ-S(P)) | ELISA | Human scFv |
| TAB-560MZ-S(P) | Human Anti-NOTCH3 Recombinant Antibody; scFv Fragment (TAB-560MZ-S(P)) | FC | Human scFv |
| TAB-561MZ-S(P) | Human Anti-NOTCH3 Recombinant Antibody; scFv Fragment (TAB-561MZ-S(P)) | FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-556MZ | Mouse Anti-NOTCH3 Recombinant Antibody (TAB-556MZ) | ELISA, Inhib, WB, Neut | Mouse IgG1, κ |
| TAB-562MZ | Anti-Human NOTCH3 Recombinant Antibody (TAB-562MZ) | ELISA, FuncS | |
| TAB-556MZ-S(P) | Mouse Anti-NOTCH3 Recombinant Antibody; scFv Fragment (TAB-556MZ-S(P)) | ELISA, WB | Mouse scFv |
| TAB-562MZ-S(P) | Anti-Human NOTCH3 Recombinant Antibody scFv Fragment (TAB-562MZ-S(P)) | ELISA | |
| TAB-563MZ-S(P) | Anti-Human NOTCH3 Recombinant Antibody scFv Fragment (TAB-563MZ-S(P)) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-557MZ | Human Anti-NOTCH3 Recombinant Antibody (TAB-557MZ) | Inhib, Neut | Chimeric (mouse/human) IgG1 |
| TAB-557MZ-S(P) | Mouse Anti-NOTCH3 Recombinant Antibody; scFv Fragment (TAB-557MZ-S(P)) | ELISA, WB | Mouse scFv |
| TAB-557MZ-F(E) | Human Anti-NOTCH3 Recombinant Antibody; Fab Fragment (TAB-557MZ-F(E)) | ELISA, WB | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-558MZ | Human Anti-NOTCH3 Recombinant Antibody (TAB-558MZ) | Inhib, Neut | Humanized IgG1 |
| TAB-558MZ-S(P) | Human Anti-NOTCH3 Recombinant Antibody; scFv Fragment (TAB-558MZ-S(P)) | ELISA, WB | Humanized scFv |
| TAB-558MZ-F(E) | Human Anti-NOTCH3 Recombinant Antibody; Fab Fragment (TAB-558MZ-F(E)) | ELISA, WB | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0282-YC-S(P) | Mouse Anti-NOTCH3 Recombinant Antibody (clone 122M23); scFv Fragment | ELISA, IHC, WB | Mouse scFv |
| HPAB-0283-YC-S(P) | Mouse Anti-NOTCH3 Recombinant Antibody; scFv Fragment (HPAB-0283-YC-S(P)) | ELISA, IHC, WB | Mouse scFv |
| HPAB-0284-YC-S(P) | Mouse Anti-NOTCH3 Recombinant Antibody; scFv Fragment (HPAB-0284-YC-S(P)) | ELISA, IHC, WB | Mouse scFv |
| HPAB-1010WJ-S(P) | Human Anti-NOTCH3 Recombinant Antibody (clone N3WCR3P1B12); scFv Fragment | ELISA, WB, FC | Human scFv |
| HPAB-1011WJ-S(P) | Human Anti-NOTCH3 Recombinant Antibody (clone hN3S2_160); scFv Fragment | ELISA, WB, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0282-YC-F(E) | Mouse Anti-NOTCH3 Recombinant Antibody (clone 122M23); Fab Fragment | ELISA, IHC, WB | Mouse Fab |
| HPAB-0283-YC-F(E) | Mouse Anti-NOTCH3 Recombinant Antibody; Fab Fragment (HPAB-0283-YC-F(E)) | ELISA, IHC, WB | Mouse Fab |
| HPAB-0284-YC-F(E) | Mouse Anti-NOTCH3 Recombinant Antibody; Fab Fragment (HPAB-0284-YC-F(E)) | ELISA, IHC, WB | Mouse Fab |
| HPAB-M0577-YC-F(E) | Human Anti-NOTCH3 Recombinant Antibody; Fab Fragment (HPAB-M0577-YC-F(E)) | FC, WB, Inhib | Human Fab |
| HPAB-0754-WJ-F(E) | Human Anti-NOTCH3 Recombinant Antibody (clone N37); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0577-YC | Human Anti-NOTCH3 Recombinant Antibody (HPAB-M0577-YC) | FC, WB, Inhib, FuncS | Human IgG1, κ |
| HPAB-M0578-YC | Human Anti-NOTCH3 Recombinant Antibody (HPAB-M0578-YC) | FC, WB, Inhib | Human IgG |
| HPAB-1010WJ | Human Anti-NOTCH3 Recombinant Antibody (clone N3WCR3P1B12) | ELISA, WB, FC | Human IgG |
| HPAB-1011WJ | Human Anti-NOTCH3 Recombinant Antibody (clone hN3S2_160) | ELISA, WB, FC | Human IgG |
| VS7-0425-WR490 | Mouse Anti-Notch3 Recombinant Antibody (clone 2E4D11) | WB, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC81 | Rabbit Anti-NOTCH3 Antibody (VS-0924-YC81) - Cancer Stem Cell Marker | IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY161 | Human Anti-NOTCH3 scFv-Fc Chimera (VS-0425-FY161) | ELISA, FC, WB | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC122 | Recombinant Anti-NOTCH3 Vesicular Antibody, EV Displayed (VS-0425-YC122) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4910 | Anti-NOTCH3 Immunohistochemistry Kit | IHC |
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