Human Anti-NOTCH3 scFv-Fc Chimera (VS-0425-FY161)
CAT#: VS-0425-FY161
NOTCH3 is the third member of the NOTCH receptor family mentioned here, with distinct expression patterns and roles, including involvement in vascular smooth muscle cell development and diseases like CADASIL. An anti-NOTCH3 scFv-FC can be utilized to investigate or target NOTCH3 function.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & actin filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44392/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong nuclear and moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5393/15972_A_8_5.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (AF22, MCF7, RT4, U-87 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000074181-NOTCH3
❮
❯
Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human NOTCH3
- Species Reactivity
- Human
- Applications
- ELISA, Flow Cytometry, Western Blot
- Related Disease
- Cerebral Arteriopathy, Autosomal Dominant, With Subcortical Infarcts And Leukoencephalopathy, Type 1 and Lateral Meningocele Syndrome
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- Notch 3; Neurogenic Locus Notch Homolog Protein 3; Notch (Drosophila) Homolog 3; Notch Homolog 3 (Drosophila); Notch Homolog 3; CADASIL1
- Gene ID
- 4854
- UniProt ID
- Q9UM47
- Long Name
- Notch 3
- Sequence Similarities
- Belongs to the NOTCH family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form.
Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC).
Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT).
This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
- Protein Refseq
- XP_005259981.1; XP_054177079.1; NP_000426.2
- Function
- Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination.
Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0425-FY161. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Diseases
Breast Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "NOTCH3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1929z | Mouse Anti-NOTCH3 Recombinant Antibody (clone 22H2) | WB, ELISA | Mouse IgG2a |
| HPAB-0282-YC | Mouse Anti-NOTCH3 Recombinant Antibody (clone 122M23) | ELISA, IHC, WB | Mouse IgG |
| HPAB-0283-YC | Mouse Anti-NOTCH3 Recombinant Antibody (HPAB-0283-YC) | ELISA, IHC, WB | Mouse IgG |
| HPAB-0284-YC | Mouse Anti-NOTCH3 Recombinant Antibody (HPAB-0284-YC) | ELISA, IHC, WB | Mouse IgG |
| HPAB-M0577-YC | Human Anti-NOTCH3 Recombinant Antibody (HPAB-M0577-YC) | FC, WB, Inhib, FuncS | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-554MZ | Human Anti-NOTCH3 Recombinant Antibody (TAB-554MZ) | FuncS, Inhib, WB | Human IgG |
| TAB-555MZ | Human Anti-NOTCH3 Recombinant Antibody (TAB-555MZ) | FC, FuncS, Inhib, WB | Human IgG |
| TAB-559MZ | Anti-Human NOTCH3 Recombinant Antibody (TAB-559MZ) | FC, Inhib, FuncS | Human antibody |
| TAB-560MZ | Human Anti-NOTCH3 Recombinant Antibody (TAB-560MZ) | FC, FACS, FuncS | Human IgG1, κ |
| TAB-561MZ | Human Anti-NOTCH3 Recombinant Antibody (TAB-561MZ) | FC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-556MZ | Mouse Anti-NOTCH3 Recombinant Antibody (TAB-556MZ) | ELISA, Inhib, WB, Neut | Mouse IgG1, κ |
| TAB-562MZ | Anti-Human NOTCH3 Recombinant Antibody (TAB-562MZ) | ELISA, FuncS | |
| TAB-556MZ-F(E) | Mouse Anti-NOTCH3 Recombinant Antibody; Fab Fragment (TAB-556MZ-F(E)) | ELISA, WB | Mouse Fab |
| TAB-562MZ-F(E) | Anti-Human NOTCH3 Recombinant Antibody Fab Fragment (TAB-562MZ-F(E)) | ELISA | |
| TAB-563MZ-F(E) | Anti-Human NOTCH3 Recombinant Antibody Fab Fragment (TAB-563MZ-F(E)) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-557MZ | Human Anti-NOTCH3 Recombinant Antibody (TAB-557MZ) | Inhib, Neut | Chimeric (mouse/human) IgG1 |
| TAB-557MZ-S(P) | Mouse Anti-NOTCH3 Recombinant Antibody; scFv Fragment (TAB-557MZ-S(P)) | ELISA, WB | Mouse scFv |
| TAB-557MZ-F(E) | Human Anti-NOTCH3 Recombinant Antibody; Fab Fragment (TAB-557MZ-F(E)) | ELISA, WB | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-558MZ | Human Anti-NOTCH3 Recombinant Antibody (TAB-558MZ) | Inhib, Neut | Humanized IgG1 |
| TAB-558MZ-S(P) | Human Anti-NOTCH3 Recombinant Antibody; scFv Fragment (TAB-558MZ-S(P)) | ELISA, WB | Humanized scFv |
| TAB-558MZ-F(E) | Human Anti-NOTCH3 Recombinant Antibody; Fab Fragment (TAB-558MZ-F(E)) | ELISA, WB | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0282-YC-F(E) | Mouse Anti-NOTCH3 Recombinant Antibody (clone 122M23); Fab Fragment | ELISA, IHC, WB | Mouse Fab |
| HPAB-0283-YC-F(E) | Mouse Anti-NOTCH3 Recombinant Antibody; Fab Fragment (HPAB-0283-YC-F(E)) | ELISA, IHC, WB | Mouse Fab |
| HPAB-0284-YC-F(E) | Mouse Anti-NOTCH3 Recombinant Antibody; Fab Fragment (HPAB-0284-YC-F(E)) | ELISA, IHC, WB | Mouse Fab |
| HPAB-M0577-YC-F(E) | Human Anti-NOTCH3 Recombinant Antibody; Fab Fragment (HPAB-M0577-YC-F(E)) | FC, WB, Inhib | Human Fab |
| HPAB-M0578-YC-F(E) | Human Anti-NOTCH3 Recombinant Antibody; Fab Fragment (HPAB-M0578-YC-F(E)) | FC, WB, Inhib | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0577-YC-S(P) | Human Anti-NOTCH3 Recombinant Antibody; scFv Fragment (HPAB-M0577-YC-S(P)) | FC, WB, Inhib | Human scFv |
| HPAB-1010WJ-S(P) | Human Anti-NOTCH3 Recombinant Antibody (clone N3WCR3P1B12); scFv Fragment | ELISA, WB, FC | Human scFv |
| HPAB-1011WJ-S(P) | Human Anti-NOTCH3 Recombinant Antibody (clone hN3S2_160); scFv Fragment | ELISA, WB, FC | Human scFv |
| HPAB-1012WJ-S(P) | Human Anti-NOTCH3 Recombinant Antibody (clone N3LHCS_18); scFv Fragment | ELISA, WB, FC | Human scFv |
| HPAB-1013WJ-S(P) | Human Anti-NOTCH3 Recombinant Antibody (clone N1wc561); scFv Fragment | ELISA, WB, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC81 | Rabbit Anti-NOTCH3 Antibody (VS-0924-YC81) - Cancer Stem Cell Marker | IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC122 | Recombinant Anti-NOTCH3 Vesicular Antibody, EV Displayed (VS-0425-YC122) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4910 | Anti-NOTCH3 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: IHC, ELISA, FC, WB, ADCC, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: Neut, ELISA, WB, SPR, IF, EM
-2.png)
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
