How was this antibody produced and purified?

This is a recombinant mouse antibody expressed in mammalian cells using chemically defined culture media. It was purified by affinity chromatography to ensure high purity (>95% as determined by SDS-PAGE), making it suitable for sensitive assays like Western Blotting.

What is the function of the PCSK9 protein?

PCSK9 (Proprotein convertase subtilisin/kexin type 9) plays a key role in cholesterol and fatty acid metabolism. It binds to LDL receptors and promotes their degradation. This antibody allows for the detection of PCSK9, facilitating research into hypercholesterolemia and lipid metabolism disorders.

Mouse Anti-PCSK9 Recombinant Antibody (TAB-0955CL)

CAT#: TAB-0955CL

This product is a recombinant mouse antibody that recognizes PCSK9. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.

Gene Expression

RNA Expression Figure 1 RNA cell line category: Cell line enhanced (A-431, CACO-2, HaCaT, HeLa, RPTEC TERT1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169174-PCSK9

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Specifications

Immunogen
Human PCSK9

Host Species
Mouse

Type
Mouse IgG

Specificity
Human PCSK9 (Proprotein convertase subtilisin kexin type 9)

Species Reactivity
Human

Applications
WB

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
See the COA

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
PCSK9; proprotein convertase subtilisin/kexin type 9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3; subtilisin/kexin-like protease PC9; neural apoptosis regulated convertase 1; convertase subtilisin/kexin type 9 preproprotein

Gene ID
255738

UniProt ID
Q8NBP7

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Breast cancer biomarkers at key points during disease progression

Clear PCSK9 Bands

This recombinant mouse antibody worked very well for Western Blotting. We were able to detect human PCSK9 in our samples with high clarity. The affinity chromatography purification seems to have resulted in a very pure product.

Useful For Lipid Research

As our lab studies cholesterol metabolism, having a reliable antibody for Proprotein convertase subtilisin kexin type 9 is essential. This product specifically recognizes the target and has become a standard reagent in our protein trafficking studies.

Q&As

How was this antibody produced and purified?

A: This is a recombinant mouse antibody expressed in mammalian cells using chemically defined culture media. It was purified by affinity chromatography to ensure high purity (>95% as determined by SDS-PAGE), making it suitable for sensitive assays like Western Blotting.
What is the function of the PCSK9 protein?

A: PCSK9 (Proprotein convertase subtilisin/kexin type 9) plays a key role in cholesterol and fatty acid metabolism. It binds to LDL receptors and promotes their degradation. This antibody allows for the detection of PCSK9, facilitating research into hypercholesterolemia and lipid metabolism disorders.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-0955CL.pdf

MSDS

MSDS - TAB-0955CL.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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