Anti-Human PCSK9 VHH Single Domain Antibody (PKE2) (CAT#: TAB-0080CL-VHH)

This single domain antibody binding to PCSK9 is capable of lowering low-density lipoprotein-cholesterol (LDL-C) levels. It can be potentially used for the treatment of cardiovascular disorders.


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  • Purity:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
FuncS

Figure 1 The effect of sdAbs on wild type (WT) PCSK9-induced LDLR degradation.

Figure 1 The effect of sdAbs on wild type (WT) PCSK9-induced LDLR degradation.

Naive HuH7 cells were incubated with 1 ug/mL of purified WT PCSK9 for 24 h in the absence (Control) or presence of 100 μg/mL of various sdAbs to PCSK9. The cells were then detached and analyzed by flow cytometry for cell surface LDLR.

FuncS

Figure 2 The effect of sdAbs on WT PCSK9-induced LDLR degradation.

Figure 2 The effect of sdAbs on WT PCSK9-induced LDLR degradation.

Naive HuH7 cells were incubated with 1 μg/mL of purified WT PCSK9 for 24 h in the absence (Control) or presence of 50 μg/mL of various sdAbs to PCSK9. The cells were then detached and analyzed by flow cytometry for cell surface LDLR.

FC

Figure 3 The effect of sdAbs on WT PCSK9-induced LDLR degradation.

Figure 3 The effect of sdAbs on WT PCSK9-induced LDLR degradation.

Flow cytometry of cell surface LDLR (level of LDLR Expression.

Inhib

Figure 4 The effect of sdAbs on WT PCSK9-induced LDLR degradation.

Figure 4 The effect of sdAbs on WT PCSK9-induced LDLR degradation.

Naive HuH7 cells were incubated for 18 h in the absence [Cnt(−)] or presence of 0.7 ug/ml (˜9 nM) WT PCSK9 protein alone [Cnt(+)] or mixed with 50 ug/ml (˜3 uM) of various purified llama sdAbs.

FuncS

Figure 5 The effect of sdAbs on the gain-of-function PCSK9-D374Y-induced LDLR degradation.

Figure 5 The effect of sdAbs on the gain-of-function PCSK9-D374Y-induced LDLR degradation.

Naive Huh7 cells were incubated for 18 h in the absence [Cnt(−)] or presence of 0.5 ug/ml (˜6 nM) PCSK9-D374Y protein alone [Cnt(+)] or mixed with 50 ug/ml (˜3 uM) of various purified llama sdAbs.

FuncS

Figure 6 The effect of sdAbs on PCSK9-D374Y gain-of-function mutation-induced LDLR degradation as measured with Dil-LDL uptake assay.

Figure 6 The effect of sdAbs on PCSK9-D374Y gain-of-function mutation-induced LDLR degradation as measured with Dil-LDL uptake assay.

Naive HepG2 cells were incubated for 4 h in the absence [Cnt(−)] or presence of 0.5 μg/mL purified PCSK9-mutant D374Y [Cnt(+)]. The addition of 50 μg/mL of various sdAbs is indicated below.

FuncS

Figure 7 The effect of sdAbs on the gain-of-function PCSK9-D374Y-induced LDLR degradation.

Figure 7 The effect of sdAbs on the gain-of-function PCSK9-D374Y-induced LDLR degradation.

Naive Huh7 cells were incubated for 18 h in the absence [Cnt(−)] or presence of 0.5 ug/ml (˜6 nM) PCSK9-D374Y protein alone [Cnt(+)] or mixed with increasing concentrations of PKE2 sdAbs.


Specifications

  • Immunogen
  • Recombinant PCSK9 protein
  • Host Species
  • Llama
  • Derivation
  • Phage display library
  • Type
  • Single domain antibody
  • Specificity
  • Human
  • Clone
  • PKE2
  • Applications
  • FuncS, Inhib, FC, WB
  • Related Disease
  • Cardiovascular disorders

Applications

  • Application Notes
  • The antibody was validated for Functional Assay, Inhibition, Flow Cytometry, Western Blot. For details, refer to Published Data.

Target

  • Alternative Names
  • PCSK9; proprotein convertase subtilisin/kexin type 9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3; subtilisin/kexin-like protease PC9; neural apoptosis regulated convertase 1; convertase subtilisin/kexin type 9 preproprotein

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "PCSK9"

Single-domain Antibody

CAT Product Name Application Type
NABL-097 Recombinant Anti-human PCSK9 VHH Single Domain Antibody WB, ELISA, IHC, FC, FuncS Llama VHH
TAB-0081CL-VHH Anti-Human PCSK9 VHH Single Domain Antibody (PKF8) FuncS, Inhib, FC, WB Single domain antibody
TAB-0082CL-VHH Anti-Human PCSK9 VHH Single Domain Antibody (PKG1) FuncS, Inhib, FC, WB Single domain antibody
TAB-0083CL-VHH Anti-Human PCSK9 VHH Single Domain Antibody (P1.70) FuncS, Inhib, FC, WB Single domain antibody
TAB-0084CL-VHH Anti-Human PCSK9 VHH Single Domain Antibody (PKE1) FuncS, Inhib, FC, WB Single domain antibody

Humanized Antibody

Neutralizing Antibody

CAT Product Name Application Type
NEUT-1826CQ Recombinant Human Anti-PCSK9 Antibody, Unconjugated Neut IgG1

Rabbit Monoclonal Antibody

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-758 Afuco™ Anti-PCSK9 ADCC Recombinant Antibody (Lodelcizumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody
AFC-TAB-H10 Afuco™ Anti-PCSK9 ADCC Recombinant Antibody (Bococizumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS ADCC enhanced antibody
AFC-TAB-H59 Afuco™ Anti-PCSK9 ADCC Recombinant Antibody (Ralpancizumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody
AFC-TAB-H31 Afuco™ Anti-PCSK9 Recombinant Antibody (AFC-TAB-H31), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF Human IgG1, λ

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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