Anti-Human PCSK9 VHH Single Domain Antibody (PKF8) (CAT#: TAB-0081CL-VHH)

This single domain antibody binding to PCSK9 is capable of lowering low-density lipoprotein-cholesterol (LDL-C) levels. It can be potentially used for the treatment of cardiovascular disorders.

  • Published Data
  • Datasheet
  • MSDS
  • COA
FuncS

Figure 1 The effect of sdAbs on wild type (WT) PCSK9-induced LDLR degradation.

Naive HuH7 cells were incubated with 1 ug/mL of purified WT PCSK9 for 24 h in the absence (Control) or presence of 100 μg/mL of various sdAbs to PCSK9. The cells were then detached and analyzed by flow cytometry for cell surface LDLR.

FuncS

Figure 2 The effect of sdAbs on WT PCSK9-induced LDLR degradation.

Naive HuH7 cells were incubated with 1 μg/mL of purified WT PCSK9 for 24 h in the absence (Control) or presence of 50 μg/mL of various sdAbs to PCSK9. The cells were then detached and analyzed by flow cytometry for cell surface LDLR.

FC

Figure 3 The effect of sdAbs on WT PCSK9-induced LDLR degradation.

Flow cytometry of cell surface LDLR (level of LDLR Expression.

Inhib

Figure 4 The effect of sdAbs on WT PCSK9-induced LDLR degradation.

Naive HuH7 cells were incubated for 18 h in the absence [Cnt(−)] or presence of 0.7 ug/ml (˜9 nM) WT PCSK9 protein alone [Cnt(+)] or mixed with 50 ug/ml (˜3 uM) of various purified llama sdAbs.

FuncS

Figure 5 The effect of sdAbs on the gain-of-function PCSK9-D374Y-induced LDLR degradation.

Naive Huh7 cells were incubated for 18 h in the absence [Cnt(−)] or presence of 0.5 ug/ml (˜6 nM) PCSK9-D374Y protein alone [Cnt(+)] or mixed with 50 ug/ml (˜3 uM) of various purified llama sdAbs.

FuncS

Figure 6 The effect of sdAbs on PCSK9-D374Y gain-of-function mutation-induced LDLR degradation as measured with Dil-LDL uptake assay.

Naive HepG2 cells were incubated for 4 h in the absence [Cnt(−)] or presence of 0.5 μg/mL purified PCSK9-mutant D374Y [Cnt(+)]. The addition of 50 μg/mL of various sdAbs is indicated below.

FuncS

The effect of sdAbs on the gain-of-function PCSK9-D374Y-induced LDLR degradation.

Naive Huh7 cells were incubated for 18 h in the absence [Cnt(−)] or presence of 0.5 ug/ml (˜6 nM) PCSK9-D374Y protein alone [Cnt(+)] or mixed with increasing concentrations of PKF8 sdAbs.


Specifications

  • Immunogen
  • Recombinant PCSK9 protein
  • Host Species
  • Llama
  • Derivation
  • Phage display library
  • Type
  • Single domain antibody
  • Specificity
  • Human
  • Clone
  • PKF8
  • Applications
  • FuncS, Inhib, FC, WB
  • Related Disease
  • Cardiovascular disorders

Applications

  • Application Notes
  • The antibody was validated for Functional Assay, Inhibition, Flow Cytometry, Western Blot. For details, refer to Published Data.

Target

  • Alternative Names
  • PCSK9; proprotein convertase subtilisin/kexin type 9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3; subtilisin/kexin-like protease PC9; neural apoptosis regulated convertase 1; convertase subtilisin/kexin type 9 preproprotein

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "PCSK9"

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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