Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-4); Fab Fragment
CAT#: HPAB-0024-LSX-F(E)
Recombinant monoclonal antibody (clone 29-AM3-4) Fab fragment binds to SIRPαV1 and V2. Clone 29-AM4-4 is a humanized antibody that can be potentially used in the treatment studies of Acute myeloid leukemia (AML).
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Neuropil
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_B_8_5.jpg
Normal Tissue
Figure 2 Colon
Endocrine cells
Staining:Medium
Intensity: Moderate
Enterocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_A_7_3.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_A_8_8.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (fHDF/TERT166, RPTEC TERT1, SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198053-SIRPA
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Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized Fab
- Specificity
- Human SIRPα
- Species Reactivity
- Human, Mouse
- Clone
- 29-AM3-4
- Applications
- ELISA
- Related Disease
- Acute myeloid leukemia
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- SIRPA; signal-regulatory protein alpha; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs
- Gene ID
- 140885
- UniProt ID
- P78324
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Excellent Specificity
We've employed this Fab Fragment in multiple assays - its specificity in targeting SIRPA has enhanced our detection capabilities considerably. The consistent performance across different experimental conditions has been reassuring, providing us with reliable results and confidence in our ongoing immune response studies.
Invaluable for Leukemia Research
The Anti-SIRPA Fab Fragment has significantly advanced our research in acute myeloid leukemia. Its ability to block SIRPα effectively revealed crucial insights into immune evasion mechanisms used by leukemia cells. The precise blocking capacity strengthens our therapeutic strategies, making it a cornerstone of promising AML treatments.
Crucial for Immune Checkpoint Blockade Studies
We've found this Fab Fragment extremely useful in examining immune checkpoint interactions. Its clear, targeted interference with the CD47-SIRPα pathway enables us to map out immune cell responses, essential for developing checkpoint inhibitors. Its reliability across repeated experiments adds immense value to our project outcomes.
Q&As
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What is the primary application of the Anti-SIRPA Fab Fragment in research?
A: The Anti-SIRPA Fab Fragment (clone 29-AM3-4) is primarily used in ELISA to study the interaction between CD47 and SIRPα, which is a key focus in cancer immunotherapy research. This fragment format allows researchers to investigate the underlying mechanisms of immune evasion by tumors, facilitating the development of potential therapeutic strategies.
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How does the Fab Fragment format benefit studies involving SIRPA?
A: The Fab Fragment format enhances tissue penetration due to its smaller size compared to full-length antibodies, making it highly suitable for in vivo studies and imaging applications. Its ability to precisely target and block specific sites on SIRPα enables detailed examination of immune checkpoint roles in diseases, thereby aiding in therapeutic research and development.
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How does the Anti-SIRPA Fab Fragment contribute to the study of acute myeloid leukemia (AML)?
A: The Anti-SIRPA Fab Fragment is pivotal in research on AML due to its ability to block the CD47-SIRPα pathway, often utilized by cancer cells to evade immune clearance. By interrupting this pathway, the Fab fragment facilitates the exploration of potential therapies that activate macrophage-mediated phagocytosis of leukemia cells, providing insights into novel treatment options for AML.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| MOB-3950z | Mouse Anti-SIRPA Recombinant Antibody (clone 43H3) | WB, FC, ICC | Mouse IgG2b, κ |
| HPAB-N0386-YC | Human Anti-SIRPA Recombinant Antibody (HPAB-N0386-YC) | ELISA, FC | Human IgG |
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| CAT | Product Name | Application | Type |
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| TAB-453CT | Human Anti-SIRPA Recombinant Antibody (TAB-453CT) | Cyt, IF, FC | Chimeric (mouse/human) IgG4 |
| TAB-453CT-S(P) | Human Anti-SIRPA Recombinant Antibody; scFv Fragment (TAB-453CT-S(P)) | WB, ELISA | Human scFv |
| TAB-453CT-F(E) | Human Anti-SIRPA Recombinant Antibody; Fab Fragment (TAB-453CT-F(E)) | WB, ELISA | Human Fab |
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| TAB-454CT | Anti-Human SIRPA Recombinant Antibody (AM4-1) | ELISA | Human antibody |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| NEUT-1970CQ | Recombinant Rat Anti-Sirpa Antibody (P84) | FC, BL, IF, IHC, IP | IgG1, κ |
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| NEUT-1971CQ | Recombinant Mouse Anti-Sirpa Antibody (CBL650) | WB, FC, CyTOF, Neut | IgG2b |
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| HPAB-0023-LSX-S(P) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM4-1); scFv Fragment | ELISA | Humanized scFv |
| HPAB-0024-LSX-S(P) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-4); scFv Fragment | ELISA | Humanized scFv |
| HPAB-0025-LSX-S(P) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM4-5); scFv Fragment | ELISA | Humanized scFv |
| HPAB-N0386-YC-S(P) | Human Anti-SIRPA Recombinant Antibody; scFv Fragment (HPAB-N0386-YC-S(P)) | ELISA, FC | Human scFv |
| HPAB-2559LY-S(P) | Human Anti-SIRPA Recombinant Antibody; scFv Fragment (HPAB-2559LY-S(P)) | Inhib | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0023-LSX-F(E) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM4-1); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0025-LSX-F(E) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM4-5); Fab Fragment | ELISA | Humanized Fab |
| HPAB-2559LY-F(E) | Human Anti-SIRPA Recombinant Antibody; Fab Fragment (HPAB-2559LY-F(E)) | Inhib | Human Fab |
| PABJ-0084-F(E) | Human Anti-SIRPA Recombinant Antibody Fab Fragment (PABJ-0084-F(E)) | ELISA, Block | Human Fab |
| PABJ-0085-F(E) | Human Anti-SIRPA Recombinant Antibody Fab Fragment (PABJ-0085-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-080CN | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-35) | ELISA, Inhib, FuncS | Human IgG |
| NS-080CN-F(E) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-35); Fab Fragment | ELISA | Human Fab |
| NS-080CN-S(P) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-35); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
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| VS-0724-YC1462 | AbPlus™ Anti-SIRPA Magnetic Beads (VS-0724-YC1462) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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| VS-0325-FY86 | Human Anti-SIRPA (clone 29-AM3-35) scFv-Fc Chimera | ELISA, Inhib | Human IgG1, scFv-Fc |
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| VS-0425-YC143 | Recombinant Anti-SIRPA Vesicular Antibody, EV Displayed (VS-0425-YC143) | Cyt, ELISA, FC, Cell-uptake |
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| VS-0525-XY6599 | Anti-Mouse SIRPA Immunohistochemistry Kit | IHC | |
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