Human Anti-SIRPA (clone 29-AM3-35) scFv-Fc Chimera
CAT#: VS-0325-FY86
An anti-SIRPA scFv-Fc antibody is a recombinant antibody that combines a single-chain variable fragment (scFv) with the Fc region of an antibody. This antibody is engineered to bind to SIRPα, a protein that plays a role in tumor immune escape.
Gene Expression
Normal Tissue
Figure 1 Cerebral cortex
Neuropil
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_B_8_5.jpg
Normal Tissue
Figure 2 Colon
Endocrine cells
Staining:Medium
Intensity: Moderate
Enterocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_A_7_3.jpg
Normal Tissue
Figure 3 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2776/6761_A_8_8.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (fHDF/TERT166, RPTEC TERT1, SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198053-SIRPA
❮
❯
Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- 29-AM3-35 specifically binds to the two most common variants of the human SIRPα IgV domain (V1 and V2).
- Species Reactivity
- Human
- Clone
- 29-AM3-35
- Applications
- ELISA, Inhibition
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Signal Regulatory Protein Alpha; CD172 Antigen-Like Family Member A; Inhibitory Receptor SHPS-1; Macrophage Fusion Receptor; PTPNS1; SHPS1; SIRP; P84; BIT; MFR; Brain-Immunoglobulin-Like Molecule With Tyrosine-Based Activation Motifs; Brain Ig-Like Molecule With Tyrosine-Based Activation Motifs; Protein Tyrosine Phosphatase, Non-Receptor Type Substrate 1; Tyrosine-Protein Phosphatase Non-Receptor Type Substrate 1; Tyrosine Phosphatase SHP Substrate 1; Signal-Regulatory Protein Alpha-1
- Gene ID
- 140885
- UniProt ID
- P78324
- Long Name
- Signal Regulatory Protein Alpha
- Cellular Localization
- Membrane
- Post Translation Modifications
- N-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF.
Dephosphorylated by PTPN11.
Glycosylation at Asn110, Asn245, Asn270, Asn292, and Asn319
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect SHPS1_HUMAN (650)
Glycosylation from GlyGen 10 sites, 14 N-linked glycans (4 sites), 1 O-linked glycan (3 sites)
- Protein Refseq
- XP_005260727.1; XP_011527475.1; XP_024307604.1; XP_047295871.1; XP_047295872.1
- Function
- Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane.
Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment.
May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity).
Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0325-FY86. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone 29-AM3-35"
- CAT
- Product Name
See other products for "Sirpa"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1201-VHH | Recombinant Anti-Mouse Sirpa VHH Single Domain Antibody | IP, FC, ChiP, Neut | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3950z | Mouse Anti-SIRPA Recombinant Antibody (clone 43H3) | WB, FC, ICC | Mouse IgG2b, κ |
| MOB-1381CT | Recombinant Mouse anti-Rat SIRPA Monoclonal antibody (FE0) | FC, IHC-Fr, IP, WB | |
| HPAB-2559LY | Human Anti-SIRPA Recombinant Antibody (HPAB-2559LY) | Inhib | Human IgG4 |
| FAMAB-1629CQ | Mouse Anti-SIRPA Recombinant Antibody (clone OX41) | FuncS, IP, WB, ELISA, IF, IHC | Mouse IgG2a |
| VS-0224-XY115 | Mouse Anti-SIRPA Recombinant Antibody (clone 03) | ELISA, WB, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-453CT | Human Anti-SIRPA Recombinant Antibody (TAB-453CT) | Cyt, IF, FC | Chimeric (mouse/human) IgG4 |
| TAB-453CT-S(P) | Human Anti-SIRPA Recombinant Antibody; scFv Fragment (TAB-453CT-S(P)) | WB, ELISA | Human scFv |
| TAB-453CT-F(E) | Human Anti-SIRPA Recombinant Antibody; Fab Fragment (TAB-453CT-F(E)) | WB, ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-454CT | Anti-Human SIRPA Recombinant Antibody (AM4-1) | ELISA | Human antibody |
| TAB-454CT-S(P) | Anti-Human SIRPA Recombinant Antibody scFv Fragment (AM4-1) | ELISA | Human antibody |
| TAB-454CT-F(E) | Anti-Human SIRPA Recombinant Antibody Fab Fragment (AM4-1) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0465MZ | Chicken Anti-PTPNS1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1970CQ | Recombinant Rat Anti-Sirpa Antibody (P84) | FC, BL, IF, IHC, IP | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1971CQ | Recombinant Mouse Anti-Sirpa Antibody (CBL650) | WB, FC, CyTOF, Neut | IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4429 | Hi-Affi™ Recombinant Rabbit Anti-SIRPA Monoclonal Antibody (SI522DS) | ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0023-LSX-S(P) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM4-1); scFv Fragment | ELISA | Humanized scFv |
| HPAB-2559LY-S(P) | Human Anti-SIRPA Recombinant Antibody; scFv Fragment (HPAB-2559LY-S(P)) | Inhib | Human scFv |
| PABJ-0078-S(P) | Human Anti-SIRPA Recombinant Antibody scFv Fragment (PABJ-0078-S(P)) | ELISA | Human scFv |
| PABJ-0084-S(P) | Human Anti-SIRPA Recombinant Antibody scFv Fragment (PABJ-0084-S(P)) | ELISA, Block | Human scFv |
| PABJ-0085-S(P) | Human Anti-SIRPA Recombinant Antibody scFv Fragment (PABJ-0085-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-080CN | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-35) | ELISA, Inhib, FuncS | Human IgG |
| NS-080CN-F(E) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-35); Fab Fragment | ELISA | Human Fab |
| NS-080CN-S(P) | Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-35); scFv Fragment | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0386-YC-F(E) | Human Anti-SIRPA Recombinant Antibody; Fab Fragment (HPAB-N0386-YC-F(E)) | ELISA, FC | Human Fab |
| HPAB-N0387-YC-F(E) | Mouse Anti-SIRPA Recombinant Antibody; Fab Fragment (HPAB-N0387-YC-F(E)) | ELISA, FC | Mouse Fab |
| HPAB-N0388-YC-F(E) | Mouse Anti-SIRPA Recombinant Antibody; Fab Fragment (HPAB-N0388-YC-F(E)) | ELISA, FC | Mouse Fab |
| HPAB-2559LY-F(E) | Human Anti-SIRPA Recombinant Antibody; Fab Fragment (HPAB-2559LY-F(E)) | Inhib | Human Fab |
| PABJ-0078-F(E) | Human Anti-SIRPA Recombinant Antibody Fab Fragment (PABJ-0078-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1462 | AbPlus™ Anti-SIRPA Magnetic Beads (VS-0724-YC1462) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC143 | Recombinant Anti-SIRPA Vesicular Antibody, EV Displayed (VS-0425-YC143) | Cyt, ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6598 | Anti-SIRPA Immunohistochemistry Kit | IHC | |
| VS-0525-XY6599 | Anti-Mouse SIRPA Immunohistochemistry Kit | IHC | |
| VS-0525-XY6600 | Anti-Rat SIRPA Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, IP, FC, FuncS, Neut, IF, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, IHC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: WB, IF, FuncS
-YJ5.png)
Application: WB, ELISA, FC, IHC, IP
-5.jpg)
Application: ELISA, Inhib, FuncS
Application: ELISA, WB, FC, IHC, IP
Application: ELISA, FuncS, Neut, IF, WB, EM, Inhib, IHC
Application: ELISA, WB
Application: IF, ICC, WB, IHC-P, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
