Human Anti-SIRPA Recombinant Antibody (TAB-453CT) (CAT#: TAB-453CT)
This product is a recombinant human antibody that recognizes SIRPA. The antibody was purified by affinity chromatography.
Specific Inquiry
WB
Figure 1 Anti-Human SIRPA Antibody (TAB-453CT) in WB
Western blot analysis of TAB-453CT was performed by loading Human SIRPA Protein (His Tag) in reduced condition Lane 1, Lane 2, Lane 3 (0.1 μg 0.3 μg 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-453CT (2 μg/mL) and HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
DB
Figure 2 Anti-Human SIRPA Antibody (TAB-453CT) in DB
Dot Blot analysis of TAB-453CT was performed by coating with Human SIRPA Protein (His Tag).
TAB-453CT incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG as a secondary antibody (1: 6000)
ELISA
Figure 3 Anti-Human SIRPA Antibody (TAB-453CT) in ELISA
ELISA analysis of TAB-453CT was performed by coating with Human SIRPA Protein (His Tag). Then blocked with BSA and incubated with TAB-453CT. The HRP-conjugated goat anti-human IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Type
- Chimeric (mouse/human) IgG4
- Specificity
- Human
- Species Reactivity
- Human
- Applications
- Cyt, IF, FC
- Related Disease
- A wide range of human cancers
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- SIRPA; signal-regulatory protein alpha; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; anti-SIRPA
- Gene ID
- 140885
- UniProt ID
- P78324
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1201-VHH | Recombinant Anti-Mouse Sirpa VHH Single Domain Antibody | IP, FC, ChiP, Neut | Llama VHH |
Customer Reviews and Q&As
Customer Review
Q&As
Enhances Immunotherapy Exploration
Our lab has seen increased clarity in studying immune checkpoint pathways using this antibody. It provides crucial insights into SIRPA interactions, opening pathways for potential cancer immunotherapies that are now being investigated further.
Robust and Versatile
We've applied this antibody across various assay platforms with robust performance. Its versatility in applications from immunofluorescence to flow cytometry supports a broad range of experimental needs in our lab.
Ideal for Cancer Research
This antibody has been ideal for exploring SIRPA's role in cancer. It provides clarity in studying immune evasion strategies by tumors, crucial for designing immune-based interventions with therapeutic potential.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-453CT, RRID: AB_3111953)
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Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
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Application: ELISA, FuncS, IHC, IF, FC, ADCC
Application: WB, ELISA, IF, FuncS, Apop, BA
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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