Human Anti-SIRPA Recombinant Antibody (clone 29-AM3-35) (CAT#: NS-080CN)

Figure 1 The binding inhibition by three anti-SIRPa antibody format compounds (AM3-35, AM4-5 and AM4-1) of binding between CD47-Fc and hSIRPα V2 expressed on mouse macrophages.

Figure 1 Anti-SIRPA Recombinant Antibody (NS-080CN) in SDS-PAGE

SDS-PAGE analysis of NS-080CN in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180 kDa.

Figure 2 Anti-SIRPA Recombinant Antibody (NS-080CN) in WB

Western blot analysis of NS-080CN was performed by loading human SIRPA protein (His Tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with NS-080CN and HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (0.8 μg)

Figure 3 Anti-SIRPA Recombinant Antibody (NS-080CN) in DB

Dot Blot analysis of NS-080CN was performed by coating with human SIRPA protein (His Tag).

NS-080CN incubation concentration: 2 μg/mL.
HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000)

Figure 4 Anti-SIRPA Recombinant Antibody (NS-080CN) in ELISA

ELISA analysis of NS-080CN was performed by coating with human SIRPA protein (His Tag). Then blocked with BSA and incubated with NS-080CN. The HRP conjugated Anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.