Anti-Human SIRPA Recombinant Antibody (AM4-1) (CAT#: TAB-454CT)
Recombinant monoclonal antibody AM4-1 specifically binds to SIRPα and can block engagement of SIRPa by CD47The antibody can be potentially used in treating hematological cancer.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
SDS-PAGE
Figure 1 Anti-SIRPA Recombinant Antibody (TAB-454CT) in SDS-PAGE
SDS-PAGE analysis of TAB-454CT in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180 kDa.
SEC-HPLC
Figure 2 Anti-SIRPA Recombinant Antibody (TAB-454CT) in SEC-HPLC
SEC-HPLC analysis of TAB-454CT shows that the purity is more than 95%.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
WB
Figure 3 Anti-SIRPA Recombinant Antibody (TAB-454CT) in WB
Western blot analysis of TAB-454CT was performed by loading human SIRPA protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-454CT and HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.6 μg)
DB
Figure 4 Anti-SIRPA Recombinant Antibody (TAB-454CT) in DB
Dot Blot analysis of TAB-454CT was performed by coating with human SIRPA protein (His tag).
TAB-454CT incubation concentration: 2 μg/mL.
HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000)
ELISA
Figure 5 Anti-SIRPA Recombinant Antibody (TAB-454CT) in ELISA
ELISA analysis of TAB-454CT was performed by coating with human SIRPA protein (His tag). Then blocked with BSA and incubated with TAB-454CT. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Host Species
- Human
- Derivation
- Library F
- Type
- Human antibody
- Specificity
- Human
- Species Reactivity
- Human
- Clone
- AM4-1
- Applications
- Used for immunoassay techniques such as: Enzyme-linked Immunosorbent Assay
- Related Disease
- Hematologic cancers
Target
- Alternative Names
- SIRPA; signal-regulatory protein alpha; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; anti-SIRPA
- Gene ID
- 140885
- UniProt ID
- P78324
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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|---|---|---|---|
| NAB-1201-VHH | Recombinant Anti-Mouse Sirpa VHH Single Domain Antibody | IP, FC, ChiP, Neut | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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