Hi-Affi™ Recombinant Rabbit Anti-SMAD4 Monoclonal Antibody (DS3323AB)
CAT#: MOR-3323
Recombinant Rabbit anti-SMAD4 monoclonal antibody for WB, IHC-P, ICC, FC, IP, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_6_6.jpg
Normal Tissue
Figure 8 Placenta
Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Trophoblastic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19154/43500_A_2_7.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141646-SMAD4
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Specifications
- Immunogen
- Synthetic peptide corresponding to residues in the C-terminus of human SMAD4.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Human
- Clone
- DS3323AB
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunohistochemistry (Paraffin); Immunocytochemistry; Flow Cytometry; Immunoprecipitation
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
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Related Diseases
Colorectal Cancer
Gastric Cancer
Pancreatic Cancer
AGE-RAGE Signaling Pathway in Diabetic Complications
Related Signaling Pathways
JAK-STAT Signaling Pathway
TGF-β Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "SMAD4"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0816CT | Recombinant Mouse anti-Human SMAD4 Monoclonal antibody (TNE57 (tbnf bt EDT-57)) | ChIP, FC, ICC, IF, IP, WB | |
| MRO-2194-CN | Rabbit Anti-SMAD4 Polyclonal Antibody (MRO-2194-CN) | IF, IHC, FC | Rabbit IgG |
| VS3-FY1359 | Recombinant Rabbit Anti-SMAD4 Antibody (clone R05-1I7) | WB, ICC, IF, IP | Rabbit IgG |
| VS7-0425-WR879 | Mouse Anti-SMAD4 Monoclonal Antibody (clone 4G1C6) | WB, IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR880 | Mouse Anti-SMAD4 Monoclonal Antibody (VS7-0425-WR880) | WB, IHC-P, IF, ICC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-4736 | Hi-Affi™ Recombinant Rabbit Anti-SMAD4 Monoclonal Antibody (TH250DS) | WB, IF, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC832 | AbPlus™ Anti-SMAD4 Magnetic Beads (VS-0724-YC832) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY201 | Human Anti-SMAD4 scFv-Fc Chimera (VS-0325-FY201) | ELISA, IF | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2107 | Anti-SMAD4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6721 | Anti-Mouse SMAD4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6720 | Anti-Human SMAD4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6722 | Anti-Rat SMAD4 Immunohistochemistry Kit | IHC |
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