Recombinant Anti-TLR2 Intrabody [(D-Arg)9] (CAT#: IAB-B030(A))
Recombinant anti-TLR2 intrabody linked to (D-Arg)9 works within cells towards TLR2, expressed in CHO cells. Intracellular antibodiess expressed and retained inside the endoplasmatic reticulum (ER-intrabodies) are applied to block translocation of secreted and cell surface molecules from the ER to the cell surface resulting in functional inhibition of the target protein. since cell activation via TLR2 and other pattern recognition receptors (PRRs) contributes to sepsis pathology and chronic inflammation both relying on overamplification of an immune response.
Specific Inquiry
Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60231/1551_D10_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60231/1551_D10_1_selected.jpg
Figure 2 RNA cell line category: Cell line enhanced (HL-60, HSkMC, hTCEpi, NB-4, RPTEC TERT1, RT4, THP-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000137462-TLR2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000137462-TLR2
Specifications
- Immunogen
- Toll-like receptor (TLR) 2
- Host Species
- murine
- Type
- scFv-(D-Arg)9
- Cell Penetrating Peptide
- (D-Arg)9
It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
- Applications
- Formats of immunological tests: Immunocytochemistry; Neutralization; Functional studies
Product Property
- Purity
- >97%, by SDS-PAGE under reducing conditions
- Storage
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Target
- Alternative Names
- CD282; CD282 antigen; TIL 4; TLR 2; TLR2; TLR2_HUMAN; Toll like receptor 2; Toll like receptor 2 precursor; Toll-like receptor 2; Toll/interleukin 1 receptor like 4; Toll/interleukin 1 receptor like protein 4; Toll/interleukin receptor like protein 4; Tol
Related Resources
NLR Signaling Pathway
TLR Signaling Pathway
Rheumatoid Arthritis
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1393z | Mouse Anti-TLR2 Recombinant Antibody (clone 23C7) | WB, FC, IF, IHC, FuncS | Mouse IgG2a, κ |
| FN-140CQ | Human Anti-TLR2 Recombinant Antibody (clone CBL-1309) | FuncS, Block | Human IgG1 |
| FN-171CQ | Rat Anti-Tlr2 Recombinant Antibody (clone 6C2) | FC, FuncS, IF, Neut | Rat IgG2b, κ |
| VS3-FY1457 | Recombinant Rabbit Anti-Tlr2 Antibody (clone R06-8B6) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS-0723-WK9 | Human Anti-TLR2 Recombinant Antibody (clone Tomaralimab) | FC | Chimeric (mouse/human) IgG4 |
Intrabody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B030(G) | Recombinant Anti-TLR2 Intrabody [+36 GFP] | ELISA, WB, ICC, FuncS | scFv-(+36GFP) |
| IAB-B030(T) | Recombinant Anti-TLR2 Intrabody [Tat] | IF, FC, WB, FuncS | scFv-Tat |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-268CQ | Mouse Anti-TLR2 Recombinant Antibody (TAB-268CQ) | FC, IHC, IF, WB | Mouse IgG1, κ |
| TAB-268CQ-S(P) | Mouse Anti-TLR2 Recombinant Antibody; scFv Fragment (TAB-268CQ-S(P)) | ELISA, FC, WB, IP | Mouse scFv |
| TAB-268CQ-F(E) | Mouse Anti-TLR2 Recombinant Antibody; Fab Fragment (TAB-268CQ-F(E)) | ELISA, FC, WB, IP | Mouse Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-269CQ | Human Anti-TLR2 Recombinant Antibody (TAB-269CQ) | ELISA, FC, FuncS, IP, Inhib | Humanized IgG1, κ |
| TAB-269CQ-S(P) | Human Anti-TLR2 Recombinant Antibody; scFv Fragment (TAB-269CQ-S(P)) | ELISA, FC, WB, IP | Humanized scFv |
| TAB-269CQ-F(E) | Human Anti-TLR2 Recombinant Antibody; Fab Fragment (TAB-269CQ-F(E)) | ELISA, FC, WB, IP | Humanized Fab |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0588MZ | Chicken Anti-TLR2 Polyclonal IgY | WB | Chicken antibody |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2031CQ | Mouse Anti-TLR2 Recombinant Antibody (clone TL2.1) | FC, IP, Block, IHC, WB | Mouse IgG2a, κ |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2032CQ | Mouse Anti-Tlr2 Recombinant Antibody (clone C9A12) | Neut | Mouse IgG2a |
| NEUT-2033CQ | Human Anti-TLR2 Recombinant Antibody (clone B4H2) | Neut, FC | Human IgA2 |
| NEUT-2034CQ | Mouse Anti-TLR2 Recombinant Antibody (clone CBL627) | FC, CyTOF, Neut | Mouse IgG2b |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3585 | Rabbit Anti-TLR2 Recombinant Antibody (clone DS3585AB) | WB, ICC, IP | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0052-YC-S(P) | Human Anti-TLR2 Recombinant Antibody; scFv Fragment (HPAB-0052-YC-S(P)) | Neut | Humanized scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0052-YC-F(E) | Human Anti-TLR2 Recombinant Antibody; Fab Fragment (HPAB-0052-YC-F(E)) | Neut | Humanized Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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