Recombinant Anti-TLR2 Intrabody [Tat]
CAT#: IAB-B030(T)
Recombinant anti-TLR2 intrabody in fusion with HIV Tat works within cells towards TLR2, expressed in CHO cells. Intracellular antibodiess expressed and retained inside the endoplasmatic reticulum (ER-intrabodies) are applied to block translocation of secreted and cell surface molecules from the ER to the cell surface resulting in functional inhibition of the target protein. since cell activation via TLR2 and other pattern recognition receptors (PRRs) contributes to sepsis pathology and chronic inflammation both relying on overamplification of an immune response.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60231/1551_D10_1_selected.jpg
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Specifications
- Immunogen
- Toll-like receptor (TLR) 2
- Host Species
- murine
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Applications
- Used for immunoassay techniques such as: Immunofluorescence; Flow Cytometry; Western blot; Functional studies
Product Property
- Purity
- >95% as determined by analysis by RP-HPLC
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- CD282; CD282 antigen; TIL 4; TLR 2; TLR2; TLR2_HUMAN; Toll like receptor 2; Toll like receptor 2 precursor; Toll-like receptor 2; Toll/interleukin 1 receptor like 4; Toll/interleukin 1 receptor like protein 4; Toll/interleukin receptor like protein 4; Tol
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Related Signaling Pathways
NLR Signaling Pathway
TLR Signaling Pathway
Related Diseases
Rheumatoid Arthritis
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-269CQ | Human Anti-TLR2 Recombinant Antibody (TAB-269CQ) | ELISA, FC, FuncS, IP, Inhib | Humanized IgG1, κ |
| TAB-269CQ-S(P) | Human Anti-TLR2 Recombinant Antibody; scFv Fragment (TAB-269CQ-S(P)) | ELISA, FC, WB, IP | Humanized scFv |
| TAB-269CQ-F(E) | Human Anti-TLR2 Recombinant Antibody; Fab Fragment (TAB-269CQ-F(E)) | ELISA, FC, WB, IP | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2351MZ | Recombinant Mouse Anti-Human TLR2 Antibody (clone 22H8) | ELISA, FC, IP | Mouse antibody |
| HPAB-0052-YC | Human Anti-TLR2 Recombinant Antibody (HPAB-0052-YC) | Neut | Humanized IgG |
| VS7-0425-WR1000 | Mouse Anti-TLR2 Recombinant Antibody (clone 8F11G10) | WB | Mouse IgG1 |
| VS7-0425-WR1001 | Mouse Anti-TLR2 Recombinant Antibody (clone 7G5F4) | ICC | Mouse IgG1 |
| VS7-0425-WR1002 | Mouse Anti-TLR2 Monoclonal Antibody (clone 2D6) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0588MZ | Chicken Anti-TLR2 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2031CQ | Mouse Anti-TLR2 Recombinant Antibody (clone TL2.1) | FC, IP, Block, IHC, WB | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2032CQ | Mouse Anti-Tlr2 Recombinant Antibody (NEUT-2032CQ) | Neut | Mouse IgG2a |
| NEUT-2033CQ | Human Anti-TLR2 Recombinant Antibody (NEUT-2033CQ) | Neut, FC | Human IgA2 |
| NEUT-2034CQ | Mouse Anti-TLR2 Recombinant Antibody (clone CBL627) | FC, CyTOF, Neut | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3585 | Rabbit Anti-TLR2 Recombinant Antibody (clone DS3585AB) | WB, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0052-YC-S(P) | Human Anti-TLR2 Recombinant Antibody; scFv Fragment (HPAB-0052-YC-S(P)) | Neut | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0052-YC-F(E) | Human Anti-TLR2 Recombinant Antibody; Fab Fragment (HPAB-0052-YC-F(E)) | Neut | Humanized Fab |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS-0425-FY29 | Human Anti-TLR2 scFv-Fc Chimera (VS-0425-FY29) | FC, Inhib, ELISA | Human IgG4, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC654 | Recombinant Anti-TLR2 Vesicular Antibody, EV Displayed (VS-0425-YC654) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7301 | Anti-TLR2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7302 | Anti-Human TLR2 Immunohistochemistry Kit | IHC |
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