Recombinant Anti-TLR2 Intrabody [Tat] (CAT#: IAB-B030(T))
Recombinant anti-TLR2 intrabody in fusion with HIV Tat works within cells towards TLR2, expressed in CHO cells. Intracellular antibodiess expressed and retained inside the endoplasmatic reticulum (ER-intrabodies) are applied to block translocation of secreted and cell surface molecules from the ER to the cell surface resulting in functional inhibition of the target protein. since cell activation via TLR2 and other pattern recognition receptors (PRRs) contributes to sepsis pathology and chronic inflammation both relying on overamplification of an immune response.
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Subcellular Location
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60231/1551_D10_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60231/1551_D10_1_selected.jpg
Figure 2 RNA cell line category: Cell line enhanced (HL-60, HSkMC, hTCEpi, NB-4, RPTEC TERT1, RT4, THP-1, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000137462-TLR2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000137462-TLR2
Specifications
- Immunogen
- Toll-like receptor (TLR) 2
- Host Species
- murine
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Applications
- Used for immunoassay techniques such as: Immunofluorescence; Flow Cytometry; Western blot; Functional studies
Product Property
- Purity
- >95% as determined by analysis by RP-HPLC
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- CD282; CD282 antigen; TIL 4; TLR 2; TLR2; TLR2_HUMAN; Toll like receptor 2; Toll like receptor 2 precursor; Toll-like receptor 2; Toll/interleukin 1 receptor like 4; Toll/interleukin 1 receptor like protein 4; Toll/interleukin receptor like protein 4; Tol
Related Resources
NLR Signaling Pathway
TLR Signaling Pathway
Rheumatoid Arthritis
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B030(A) | Recombinant Anti-TLR2 Intrabody [(D-Arg)9] | ICC, Neut, FuncS | scFv-(D-Arg)9 |
| IAB-B030(G) | Recombinant Anti-TLR2 Intrabody [+36 GFP] | ELISA, WB, ICC, FuncS | scFv-(+36GFP) |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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