Recombinant Mouse Anti-Integrin alpha 4 Antibody Fab Fragment (ACT-1)
CAT#: PABX-122-F (E)
Anti-Integrin alpha 4 antibody is a Mouse of Fab class that binds to an Integrin alpha 4.
Published Data
Gene Expression
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Figure 1 Nuclear localization of Act1
HBE1 cells plated on glass coverslips were left unstimulated or stimulated with 100 ng/mL IL-17A for 1h. Endogenous Act1 was immunostained with a rabbit anti-Act1 antibody, followed by an Alexa-488 conjugated secondary antibody (green) and counterstained with DAPI to visualize nuclei (blue).
Velichko, S., Zhou, X., Zhu, L., Anderson, J. D., Wu, R., & Chen, Y. (2016). A novel nuclear function for the interleukin-17 signaling adaptor protein Act1. PloS one, 11(10), e0163323.
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Figure 2 Binding of mAb ACT-1, HIV-1 gp120, and cyclic V2 peptides to RMPI8866 cells.
(A) RPMI8866 cells (0.5 x106) were incubated with 1 μg APC labeled ACT-1 (α4β7 specific antibody). Antibody binding was measured by flow cytometry and is presented as a histogram. Over 99% of the RPMI8866 cells expressed α4β7 integrin. Unstained cells (grey shading) and isotype control antibody (solid line) were used as controls. (A and C) Histograms show the binding of CHO-expressed HIV-1 gp120 (2.5 μg, blue line panel B), biotin labeled cyclic V2 TH023 peptide (1μg and 5 μg; black and orange lines, respectively; panel C), and biotin labeled cyclic V2 TH023 -fully scrambled peptide (SCR; 1 μg and 5 μg; blue line and green line; panel C) to RMPI8866 cells. Polyclonal mouse anti-gp145 antibody followed by goat anti-mouse-Texas Red was used to visualize the gp120 protein binding while the V2 peptides were visualized with neutrAvidin-PE. Cells treated with only anti-mouse-Texas Red (red line, panel B) and unstained cells (red line, panel C) were used as controls in addition to the fully scrambled V2 peptide (blue and green line, panel C). Representative histograms are shown for panels A, B, and C (n = 2).
Peachman, K. K., Karasavvas, N., Chenine, A. L., McLinden, R., Rerks-Ngarm, S., Jaranit, K., ... & Michael, N. L. (2015). Identification of new regions in HIV-1 gp120 variable 2 and 3 loops that bind to α4β7 integrin receptor. PLoS One, 10(12), e0143895.
-3.png)
Figure 3 Immunohistochemical staining of RAGE, Act1 and IL-17.
(a) The synovial tissue sections from patients with rheumatoid arthritis and osteoarthritis were stained with antibodies to RAGE, Act-1, IL-17, and H&E or an isotype-control antibody. The brown color shows the target.
Heo, Y. J., Oh, H. J., Jung, Y. O., Cho, M. L., Lee, S. Y., Yu, J. G., ... & Kim, H. Y. (2011). The expression of the receptor for advanced glycation end-products (RAGE) in RA-FLS is induced by IL-17 via Act-1. Arthritis research & therapy, 13(4), R113.
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Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/74961/1608_C10_1_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (fHDF/TERT166, HEL, JURKAT, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000115232-ITGA4
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Fab
- Specificity
- Tested positive against native Human Integrin alpha 4
- Species Reactivity
- Human
- Clone
- ACT-1
- Applications
- Neut (assay dependent)
FuncS (assay dependent)
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
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Reliable for Research Needs
I've used the Recombinant Mouse Anti-Integrin alpha 4 Antibody for my cell biology project. It binds well to my target cells and gives consistent results. I appreciate its high purity and the detailed documentation provided by Creative Biolabs.
Great Support from Provider
Creative Biolabs offers outstanding customer service, making it easy to understand how best to use their antibody products. Their technical support is responsive and very helpful.
Q&As
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How do I store the recombinant anti-Integrin alpha 4 antibody?
A: You should store this antibody in aliquots at -20°C. It's best to avoid repeated freezing and thawing to maintain its effectiveness.
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What are the purity levels of this product?
A: The purity of this antibody is greater than 95% as assessed by SDS-PAGE and HPLC analysis, ensuring reliable experimental results.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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