Mouse Anti-AFP Recombinant Antibody (VS3-CJ312)
CAT#: VS3-CJ312
This product is a mouse antibody that recognizes AFP.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10607/1816_H10_5_selected.jpg
Subcellular Location
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong positivity in plasma.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10607/25809_A_2_7_selected.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enriched (Hep G2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000081051-AFP
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Specifications
- Immunogen
- Recombinant protein of human AFP
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse and Rat AFP
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC, IF
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50% Glycerol, PBS, pH7.3.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:2000), Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200).
Target
- Alternative Names
- AFPD; FETA; HPAFP
- Gene ID
- 174
- UniProt ID
- P02771
- Sequence Similarities
- Belongs to the ALB/AFP/VDB family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated.
- Protein Refseq
- NP_001125.1
- Function
- Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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