Anti-AKT3 Immunohistochemistry Kit
CAT#: VS-0525-XY286
Designed for neuroscience and cancer studies, the AKT3 IHC kit provides high-fidelity staining. It's fully compatible with paraffin and frozen sections, offering convenience and consistency.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13090/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26441/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13090/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13090/636_F8_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26441/56592_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26441/56592_A_9_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26441/56592_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26441/56592_A_4_6.jpg
Normal Tissue
Figure 9 Skin
Fibroblasts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Melanocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26441/56592_B_8_1.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000117020-AKT3
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human
- Target
- AKT3
- Primary Antibody
- Mouse Anti-AKT3 Antibody
- Secondary Antibody
- Goat anti-Mouse Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Related Signaling Pathways
BCR Signaling Pathway
ErbB Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Ras Signaling Pathway
Related Diseases
Breast Cancer
Colorectal Cancer
Gastric Cancer
Hepatocellular Carcinoma
Non-small Cell Lung Cancer
Prostate Cancer
Endocrine Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "AKT3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-65-sdAb | Recombinant Anti-human AKT3 VHH Single Domain Antibody | Neut, ChiP, IP, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0575MZ | Mouse Anti-AKT3 Recombinant Antibody (clone CBLS-088) | ELISA, WB | Mouse IgG2a |
| ZG-0275F | Mouse Anti-AKT3 Recombinant Antibody (ZG-0275F) | WB, ELISA | Mouse IgG |
| VS3-FY58 | Recombinant Rabbit Anti-AKT3 Antibody (clone R08-8F1) | WB, ICC, IF | Rabbit IgG |
| VS7-HM70 | Mouse Anti-AKT3 Recombinant Antibody (clone 7B7A2) | FCM | Mouse IgG1 |
| VS7-HM71 | Mouse Anti-Akt3 Recombinant Antibody (clone CBL026HM) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0034MZ | Chicken Anti-AKT3 Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
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