Rabbit Anti-AKT3 Recombinant Antibody (clone K17-H), FITC
CAT#: ZG-0437U
Rabbit anti-AKT3 antibody (clone K17-H) was conjugated with FITC under optimal conditions. The FITC-conjugated antibody product displayed high specificity against the AKT3 protein of Human. And the solution is free of unconjugated FITC and unconjugated antibody.
Tested Data
Gene Expression
FC
Figure 1 Rabbit Anti-AKT3 Antibody (ZG-0437U) in FC
Profile of Akt3 expression in human blood lymphocytes analyzed by the BD FACSCanto II. Blood cells of patient with chronic lymphocytic leukemia were fixed, permeabilized and stained with anti-human Akt3 FITC (red, used 10 μl per test) or with an isotype control (black).
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13090/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26441/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13090/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/13090/636_F8_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26441/56592_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26441/56592_A_9_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26441/56592_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26441/56592_A_4_6.jpg
Normal Tissue
Figure 9 Skin
Fibroblasts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Melanocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26441/56592_B_8_1.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000117020-AKT3
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human AKT3
- Species Reactivity
- Human
- Clone
- K17-H
- Applications
- FC
- Conjugate
- FITC
- Related Disease
- Megalencephaly-Polymicrogyria-Polydactyly-Hydrocephalus Syndrome 2 and Megalencephaly-Polymicrogyria-Polydactyly-Hydrocephalus Syndrome 1.
Product Property
- Buffer
- Aqueous buffer solution containing 10mg/ml of BSA and 0.05% sodium azide.
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C, in the dark. Do not freeze.
Applications
- Application Notes
- This antibody has been used 10 µL per test for Flow Cytometry. Specific conditions should be optimized by the end user.
Target
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Related Signaling Pathways
BCR Signaling Pathway
ErbB Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Ras Signaling Pathway
Related Diseases
Breast Cancer
Colorectal Cancer
Gastric Cancer
Hepatocellular Carcinoma
Non-small Cell Lung Cancer
Prostate Cancer
Endocrine Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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