Anti-APC Antibody Prodrug, Protease Activated (VS-1025-YC12)
CAT#: VS-1025-YC12
The Anti-APC Antibody Prodrug, Protease Activated (VS-1025-YC12) is an antibody prodrug comprised of a recombinant human antibody targeting human APC, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to APC protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to APC effectively. APC (Adenomatous Polyposis Coli) is a major tumor suppressor protein primarily functioning as a key component of the Wnt signaling pathway's destruction complex. Loss-of-function mutations in the APC gene are the initiating event in over 80% of sporadic colorectal cancers and are the cause of familial adenomatous polyposis (FAP). When APC is mutated, the beta-catenin protein accumulates and translocates to the nucleus, driving uncontrolled cell proliferation and tumor initiation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13362/1401_C6_5_selected.jpg
Normal Tissue
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_B_9_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13349/165147_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134982-APC
❮
❯
Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human APC
- Target
- APC
- Species Reactivity
- Human
- Isotype
- IgG, kappa
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- Protein C, also known as autoprothrombin IIA and blood coagulation factor XIV, is a zymogen, the activated form of which plays an important role in regulating anticoagulation, inflammation, cell death, and maintaining the permeability of blood vessel walls in humans and other animals. Activated protein C (APC) performs these operations primarily by proteolytically inactivating proteins Factor Va and Factor VIIIa. APC is classified as a serine protease as it contains a residue of serine in its active site. In humans, protein C is encoded by the PROC gene, which is found on chromosome 2.
- Alternative Names
- Activated protein C; APC
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-1025-YC12. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Diseases
Breast Cancer
Colorectal Cancer
Endometrial Cancer
Gastric Cancer
Pancreatic Cancer
Prostate Cancer
Throid Cancer
Hepatocellular Carcinoma
Cushing Syndrome
Related Signaling Pathways
Canonical Wnt Signaling Pathway
JAK-STAT Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "APC"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-72 | Recombinant Anti-human APC Antibody | ELISA, WB, FC, FuncS | IgG |
| MHH-72 | Recombinant Human Anti-human APC Antibody | WB, FACS, FuncS | IgG |
| MOB-0647MZ | Mouse Anti-APC Recombinant Antibody (clone CBLS-110) | IHC-Fr | Mouse IgG1 |
| MRO-0106-CN | Rabbit Anti-APC Recombinant Antibody (clone CBACN-035) | WB, IF, IP | Rabbit IgG |
| MRO-1651-CN | Rabbit Anti-APC Polyclonal Antibody (MRO-1651-CN) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-72-F(E) | Recombinant Anti-human APC Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
| MHH-72-F(E) | Recombinant Human Anti-human APC Antibody Fab Fragment | FC, Neut, Biosensors, FuncS | Fab |
| HPAB-0095-WJ-F(E) | Human Anti-APC Recombinant Antibody; Fab Fragment (HPAB-0095-WJ-F(E)) | ELISA | Humanized Fab |
| HPAB-J0036-YC-F(E) | Mouse Anti-aPC Recombinant Antibody; Fab Fragment (HPAB-J0036-YC-F(E)) | ELISA | Mouse Fab |
| HPAB-J0037-YC-F(E) | Mouse Anti-aPC Recombinant Antibody; Fab Fragment (HPAB-J0037-YC-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-72-S(P) | Recombinant Anti-human APC Antibody scFv Fragment | IP, IF, Biosensors, FuncS | scFv |
| MHH-72-S(P) | Recombinant Human Anti-human APC Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| HPAB-0095-WJ-S(P) | Human Anti-APC Recombinant Antibody; scFv Fragment (HPAB-0095-WJ-S(P)) | ELISA | Humanized scFv |
| HPAB-J0036-YC-S(P) | Mouse Anti-aPC Recombinant Antibody; scFv Fragment (HPAB-J0036-YC-S(P)) | ELISA | Mouse scFv |
| HPAB-J0037-YC-S(P) | Mouse Anti-aPC Recombinant Antibody; scFv Fragment (HPAB-J0037-YC-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-241CT | Mouse Anti-aPC Recombinant Antibody (TAB-241CT) | Non-neutralizing | Mouse IgG2b |
| TAB-242CT | Human Anti-aPC Recombinant Antibody (TAB-242CT) | ELISA, WB | Human IgG1 |
| TAB-243CT | Human Anti-aPC Recombinant Antibody (TAB-243CT) | ELISA, WB | Human IgG1 |
| TAB-244CT | Human Anti-aPC Recombinant Antibody (TAB-244CT) | ELISA, WB | Human IgG1 |
| TAB-245CT | Human Anti-aPC Recombinant Antibody (TAB-245CT) | ELISA, WB | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0199 | Hi-Affi™ Rabbit Anti-APC Recombinant Antibody (clone DS199AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY127 | Anti-APC Immunohistochemistry Kit | IHC | |
| VS-0525-XY439 | Anti-Mouse APC Immunohistochemistry Kit | IHC | |
| VS-0525-XY438 | Anti-Human APC Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, Cyt, Stim, PP, Agonist
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, WB, BLI, SPR
Application: ELISA
Application: ELISA, Neut
Application: ELISA, FC, Neut, Inhib
Application: ELISA, FC
Application: ELISA, FC, FuncS
Application: IF, ICC, WB, IHC-P, IP
Application: WB, IHC-Fr, IHC-P, ICC, IF
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
