Anti-BRAF Antibody Prodrug, Protease Activated (VS-1025-YC14)
CAT#: VS-1025-YC14
The Anti-BRAF Antibody Prodrug, Protease Activated (VS-1025-YC14) is an antibody prodrug comprised of a recombinant human antibody targeting human BRAF, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to BRAF protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to BRAF effectively. BRAF is a serine/threonine kinase that is a key component of the RAS/MAPK signaling pathway that regulates cell growth. Mutational activation, most commonly the V600E point mutation, occurs in a significant fraction of melanomas, thyroid, and colorectal cancers, resulting in a constitutively active kinase that drives relentless cell proliferation. This frequent and dominant oncogenic mechanism makes BRAF a major target for highly effective, mutation-specific targeted therapies.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71048/1487_C12_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1328/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157764-BRAF
❮
❯
Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human BRAF
- Target
- BRAF
- Species Reactivity
- Human
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- The monoclonal antibodies specifically recognizing B-Raf mutant proteins can be used to detect whether there are mutations in the B-Raf proteins of the tissues of an animal or a human in order to provide the basis for the diagnosis and treatment of skin cancer and other various cancers clinically.
- Alternative Names
- BRAF; B-Raf proto-oncogene, serine/threonine kinase; NS7; BRAF1; RAFB1; B-RAF1; B-Raf
- Gene ID
- 673
- UniProt ID
- P15056
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RESOURCES
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Related Signaling Pathways
cAMP Signaling Pathway
MAPK Signaling Pathway
Related Diseases
Throid Cancer
Cushing Syndrome
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-122 | Recombinant Anti-human BRAF Antibody | ELISA, WB, Dot, FuncS | IgG |
| MHH-122 | Recombinant Human Anti-human BRAF Antibody | IP, FuncS | IgG |
| MOB-0876MZ | Mouse Anti-BRAF Recombinant Antibody (clone 2I23) | ELISA, IHC-P, WB | Mouse IgG1 |
| MRO-0148-CN | Rabbit Anti-BRAF Recombinant Antibody (clone CBACN-057) | WB, IHC, IP, FC | Rabbit IgG |
| MRO-0189-CN | Mouse Anti-BRAF Recombinant Antibody (clone F1-F4-E10) | WB, IHC, IF | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-122-F(E) | Recombinant Anti-human BRAF Antibody Fab Fragment | IF, Neut, FuncS | Fab |
| MHH-122-F(E) | Recombinant Human Anti-human BRAF Antibody Fab Fragment | ELISA, WB, Neut, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-122-S(P) | Recombinant Anti-human BRAF Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
| MHH-122-S(P) | Recombinant Human Anti-human BRAF Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1284CL | Anti-Human BRAF Recombinant Antibody (CCTCC C2012122) | IB, IF | |
| TAB-1284CL-S(P) | Anti-Human BRAF Recombinant Antibody scFv Fragment (CCTCC C2012122) | IB, IF | |
| TAB-1284CL-F(E) | Anti-Human BRAF Recombinant Antibody Fab Fragment (CCTCC C2012122) | IB, IF |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0379 | Hi-Affi™ Rabbit Anti-BRAF Recombinant Antibody (clone DS379AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0457 | PE-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-CN0458 | APC-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-CN0459 | FITC-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-CN0460 | B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-CN0461 | PE-B*27:05/Human BRAF (GRFGLATVK) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY32 | AbPlus™ Anti-BRAF Magnetic Beads (CBACN-057) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY56 | Rabbit Anti-NHP BRAF Recombinant Antibody (VS-1024-XY56) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY821 | Anti-BRAF Immunohistochemistry Kit | IHC | |
| VS-0525-XY822 | Anti-Mouse BRAF Immunohistochemistry Kit | IHC | |
| VS-0525-XY823 | Anti-Monkey BRAF Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC220 | SmartAb™ Recombinant Anti-BRAF pH-dependent Antibody (VS-0825-YC220) | ELISA | Human IgG |
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