Anti-Human BRAF Recombinant Antibody scFv Fragment (CCTCC C2012122)
CAT#: TAB-1284CL-S(P)
This antibody is a monoclonal Antibodies specifically recognizing B-Raf mutant proteins, and can be used to detect whether there are N581S, V600E and V600K mutations in the B-Raf proteins of the tissues of an animal or a human in order to provide the basis for the diagnosis and treatment of skin cancer and other various cancers clinically.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71048/1487_C12_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1328/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157764-BRAF
❮
❯
Specifications
- Immunogen
- Mutant B-Raf polypeptide
- Host Species
- Mouse
- Specificity
- Human
- Clone
- CCTCC C2012122
- Related Disease
- Skin cancer and other various cancers
Target
REVIEWS AND Q&AS
CITATIONS
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Related Signaling Pathways
cAMP Signaling Pathway
MAPK Signaling Pathway
Related Diseases
Throid Cancer
Cushing Syndrome
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-122-F(E) | Recombinant Anti-human BRAF Antibody Fab Fragment | IF, Neut, FuncS | Fab |
| MHH-122-F(E) | Recombinant Human Anti-human BRAF Antibody Fab Fragment | ELISA, WB, Neut, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-122-S(P) | Recombinant Anti-human BRAF Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
| MHH-122-S(P) | Recombinant Human Anti-human BRAF Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-122 | Recombinant Human Anti-human BRAF Antibody | IP, FuncS | IgG |
| ZG-053R | Mouse Anti-BRAF Recombinant Antibody (ZG-053R) | WB, ELISA, IHC | Mouse IgG |
| ZG-054R | Mouse Anti-BRAF Recombinant Antibody (ZG-054R) | WB, ELISA, IHC, IF | Mouse IgG |
| ZG-0572J | Rabbit Anti-BRAF Recombinant Antibody (clone 6G3) | ELISA, IHC | Rabbit IgG |
| VS3-QX130 | Mouse Anti-BRAF Recombinant Antibody (clone 4E1-E1-F9) | WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1284CL | Anti-Human BRAF Recombinant Antibody (CCTCC C2012122) | IB, IF | |
| TAB-1284CL-F(E) | Anti-Human BRAF Recombinant Antibody Fab Fragment (CCTCC C2012122) | IB, IF |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0379 | Hi-Affi™ Rabbit Anti-BRAF Recombinant Antibody (clone DS379AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0457 | PE-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-CN0458 | APC-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-CN0459 | FITC-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-CN0460 | B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-CN0461 | PE-B*27:05/Human BRAF (GRFGLATVK) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY32 | AbPlus™ Anti-BRAF Magnetic Beads (CBACN-057) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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| VS-1024-XY56 | Rabbit Anti-NHP BRAF Recombinant Antibody (VS-1024-XY56) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY821 | Anti-BRAF Immunohistochemistry Kit | IHC | |
| VS-0525-XY822 | Anti-Mouse BRAF Immunohistochemistry Kit | IHC | |
| VS-0525-XY823 | Anti-Monkey BRAF Immunohistochemistry Kit | IHC |
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