Recombinant Human Anti-human BRAF Antibody Fab Fragment

Name: Recombinant Human Anti-human BRAF Antibody Fab Fragment
SKU: MHH-122-F(E)
Rating: 5 (1 reviews)

CAT#: MHH-122-F(E)

Recombinant Human Antibody Fab Fragment recognizes and reacts with Human BRAF, expressed in HEK 293 cells.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line SiHa

Immunofluorescent staining of human cell line SiHa shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71048/1487_C12_3_selected.jpg

Normal Tissue Figure 2 IHC staining of human testis

Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1328/ihc_selected.jpg

Normal Tissue Figure 3 IHC staining of human cerebral cortex

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/ihc_selected.jpg

Normal Tissue Figure 4 Cerebral cortex

Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_B_7_5.jpg

Normal Tissue Figure 5 Colon

Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_7_3.jpg

Normal Tissue Figure 6 Liver

Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_4.jpg

Normal Tissue Figure 7 Kidney

Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_5.jpg

Normal Tissue Figure 8 Testis

Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_6_6.jpg

Normal Tissue Figure 9 Lymph node

Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_8.jpg

RNA Expression Figure 10 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157764-BRAF

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Specifications

Immunogen
Human B-Raf proto-oncogene, serine/threonine kinase

Host Species
Human

Derivation
Human

Type
Fab

Antibody Isotype
Fab Fragment based on human IgG1

Specificity
Tested positive against native Human B-Raf proto-oncogene, serine/threonine kinase

Species Reactivity
Human

Applications
Products can be used for: Enzyme-linked Immunosorbent Assay; Western blot; Neutralization; Functional Study

Product Property

Purity
>90% assessed by SDS-PAGE

Storage
Store at -20°C. Avoid multiple freeze/thaw cycles.

Target

Alternative Names
BRAF; B-Raf proto-oncogene, serine/threonine kinase; NS7; BRAF1; RAFB1; B-RAF1; serine/threonine-protein kinase B-raf; 94 kDa B-raf protein; proto-oncogene B-Raf; v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B

Gene ID
673

UniProt ID
P15056

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Related Signaling Pathways

cAMP Signaling Pathway

MAPK Signaling Pathway

Related Diseases

Throid Cancer

Cushing Syndrome

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MHH-122-F(E).pdf

MSDS

MSDS - MHH-122-F(E).pdf

COA

Certificate of Analysis Lookup

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