Anti-Human BRAF Recombinant Antibody (CCTCC C2012122) (CAT#: TAB-1284CL)
This antibody is a monoclonal Antibodies specifically recognizing B-Raf mutant proteins, and can be used to detect whether there are N581S, V600E and V600K mutations in the B-Raf proteins of the tissues of an animal or a human in order to provide the basis for the diagnosis and treatment of skin cancer and other various cancers clinically.
Specific Inquiry
IB
Figure 1 An immunoblotting assay map of an antibody produced by a cell line with the accession number CCTCC C2012122 that specifically binds to the B-Raf V600K protein but does not bind to the wild-type B-Raf protein.
Lane 1 contains There is a wild-type B-Raf protein expressed by E. coli. Lane 2 contains the mutant B-Raf V600K protein expressed in E. coli. Lane 3 contains the wild-type B-Raf protein expressed by HEK293T cells. Lane 4 contains the mutant B-Raf V600K protein expressed by HEK293T cells.
IF
Figure 2 The immunofluorescence assay map of the antibody produced by the cell line with the accession number CCTCC C2012112 that specifically binds to the B-Raf N581S protein but does not bind to the wild-type B-Raf protein.
The green fluorescent protein (GFP) signal indicates the expression of wild-type B-Raf (top) and mutant B-Raf N581S protein (lower), respectively. The red fluorescent signal is capable of indicating the binding of the antibody to the B-Raf protein and appears only in cells expressing B-Raf N581S protein (below).
IF
Figure 3 The antibody-specific binding of B-Raf produced by a cell line with the accession number CCTCC C2012122.
The V600K protein does not bind to the immunofluorescence assay of wild-type B-Raf protein. The green fluorescent protein (GFP) signal indicates the expression of wild-type B-Raf (top) and mutant B-Raf V600K protein (lower), respectively. The red fluorescent signal is indicative of binding of the antibody to the B-Raf protein and appears only in cells expressing the B-Raf V600K protein
Specifications
- Immunogen
- Mutant B-Raf polypeptide
- Host Species
- Mouse
- Specificity
- Human
- Clone
- CCTCC C2012122
- Applications
- IB, IF
- Related Disease
- Skin cancer and other various cancers
Applications
- Application Notes
- This antibody has been tested for use in Immunoblotting and Immunofluorescence.
Target
- Alternative Names
- BRAF; B-Raf proto-oncogene, serine/threonine kinase; NS7; BRAF1; RAFB1; B-RAF1; B-Raf
- Gene ID
- 673
- UniProt ID
- P15056
Related Resources
cAMP Signaling Pathway
MAPK Signaling Pathway
Throid Cancer
Cushing Syndrome
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-122 | Recombinant Anti-human BRAF Antibody | ELISA, WB, Dot, FuncS | IgG |
| MRO-0148-CN | Rabbit Anti-BRAF Recombinant Antibody (clone CBACN-057) | WB, IHC, IP, FC | Rabbit IgG |
| ZG-0572J | Rabbit Anti-BRAF Recombinant Antibody (clone 6G3) | ELISA, IHC | Rabbit IgG |
| VS3-FY165 | Recombinant Mouse Anti-BRAF Antibody (clone 2A10D7) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-FY166 | Recombinant Mouse Anti-BRAF Antibody (clone 3B8B12) | ELISA, WB, IHC, ICC, FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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