Anti-Human BRAF Recombinant Antibody (CCTCC C2012122)
CAT#: TAB-1284CL
This antibody is a monoclonal Antibodies specifically recognizing B-Raf mutant proteins, and can be used to detect whether there are N581S, V600E and V600K mutations in the B-Raf proteins of the tissues of an animal or a human in order to provide the basis for the diagnosis and treatment of skin cancer and other various cancers clinically.
Published Data
Gene Expression
IB
Figure 1 An immunoblotting assay map of an antibody produced by a cell line with the accession number CCTCC C2012122 that specifically binds to the B-Raf V600K protein but does not bind to the wild-type B-Raf protein.
Lane 1 contains There is a wild-type B-Raf protein expressed by E. coli. Lane 2 contains the mutant B-Raf V600K protein expressed in E. coli. Lane 3 contains the wild-type B-Raf protein expressed by HEK293T cells. Lane 4 contains the mutant B-Raf V600K protein expressed by HEK293T cells.
IF
Figure 2 The immunofluorescence assay map of the antibody produced by the cell line with the accession number CCTCC C2012112 that specifically binds to the B-Raf N581S protein but does not bind to the wild-type B-Raf protein.
The green fluorescent protein (GFP) signal indicates the expression of wild-type B-Raf (top) and mutant B-Raf N581S protein (lower), respectively. The red fluorescent signal is capable of indicating the binding of the antibody to the B-Raf protein and appears only in cells expressing B-Raf N581S protein (below).
IF
Figure 3 The antibody-specific binding of B-Raf produced by a cell line with the accession number CCTCC C2012122.
The V600K protein does not bind to the immunofluorescence assay of wild-type B-Raf protein. The green fluorescent protein (GFP) signal indicates the expression of wild-type B-Raf (top) and mutant B-Raf V600K protein (lower), respectively. The red fluorescent signal is indicative of binding of the antibody to the B-Raf protein and appears only in cells expressing the B-Raf V600K protein
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Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71048/1487_C12_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1328/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157764-BRAF
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Specifications
- Immunogen
- Mutant B-Raf polypeptide
- Host Species
- Mouse
- Specificity
- Human
- Clone
- CCTCC C2012122
- Applications
- IB, IF
- Related Disease
- Skin cancer and other various cancers
Applications
- Application Notes
- This antibody has been tested for use in Immunoblotting and Immunofluorescence.
Target
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Related Signaling Pathways
cAMP Signaling Pathway
MAPK Signaling Pathway
Related Diseases
Throid Cancer
Cushing Syndrome
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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