Rabbit Anti-NHP BRAF Recombinant Antibody (VS-1024-XY56)
CAT#: VS-1024-XY56
Harnessing the power of a rabbit-derived monoclonal antibody, this reagent is designed to specifically target BRAF, demonstrating reactivity across human, mouse, non-human primate, and rat specimens. The immunogen is a synthetic peptide corresponding to BRAF, ensuring precise and reliable detection. Ideal for Western blot (WB) applications, this antibody provides high specificity and exceptional performance in uncovering BRAF-related biological insights. Its broad reactivity and robust analytical capabilities make it a crucial tool for advancing research into BRAF-associated pathways and disorders.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71048/1487_C12_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1328/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4552/19083_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157764-BRAF
❮
❯
Specifications
- Immunogen
- Synthetic peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- BRAF
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Applications
- WB
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 0.01 M HEPES, pH 7.5, with 0.15 M NaCl, 100 µg/mL BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800
Target
REVIEWS AND Q&AS
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Related Signaling Pathways
cAMP Signaling Pathway
MAPK Signaling Pathway
Related Diseases
Throid Cancer
Cushing Syndrome
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-122 | Recombinant Anti-human BRAF Antibody | ELISA, WB, Dot, FuncS | IgG |
| MRO-0189-CN | Mouse Anti-BRAF Recombinant Antibody (clone F1-F4-E10) | WB, IHC, IF | Mouse IgG2b |
| MRO-2303-CN | Rabbit Anti-BRAF Recombinant Antibody (clone CBACN-596) | WB, IF, IP | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-122-F(E) | Recombinant Anti-human BRAF Antibody Fab Fragment | IF, Neut, FuncS | Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-122-S(P) | Recombinant Anti-human BRAF Antibody scFv Fragment | ELISA, IF, FuncS | scFv |
| MHH-122-S(P) | Recombinant Human Anti-human BRAF Antibody scFv Fragment | WB, Neut, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1284CL | Anti-Human BRAF Recombinant Antibody (CCTCC C2012122) | IB, IF | |
| TAB-1284CL-S(P) | Anti-Human BRAF Recombinant Antibody scFv Fragment (CCTCC C2012122) | IB, IF | |
| TAB-1284CL-F(E) | Anti-Human BRAF Recombinant Antibody Fab Fragment (CCTCC C2012122) | IB, IF |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0379 | Hi-Affi™ Rabbit Anti-BRAF Recombinant Antibody (clone DS379AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0457 | PE-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-CN0458 | APC-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-CN0459 | FITC-B*27:05/Human BRAF (GRFGLATEK) MHC Tetramer | FCM, IHC | |
| MHC-YF341 | B*27:05/Human BRAF (600E) (GRFGLATEK) MHC Pentamer | FCM | |
| MHC-YF342 | B*27:05/Human BRAF (600V) (GRFGLATVK) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY32 | AbPlus™ Anti-BRAF Magnetic Beads (CBACN-057) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY821 | Anti-BRAF Immunohistochemistry Kit | IHC | |
| VS-0525-XY822 | Anti-Mouse BRAF Immunohistochemistry Kit | IHC | |
| VS-0525-XY823 | Anti-Monkey BRAF Immunohistochemistry Kit | IHC |
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