AbPlus™ Anti-BRD4 Magnetic Beads (D392)
CAT#: VS-0424-XY33
The AbPlus Anti-BRD4 Magnetic Beads (D392) is an innovative affinity resin which is bound with anti-BRD4 specific antibody (D392). The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of BRD4 protein under native and denaturing conditions.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human skin
Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human colon
Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68177/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 6 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 7 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/1080_H5_1_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_B_9_5.jpg
Normal Tissue
Figure 9 Colon
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_3.jpg
Normal Tissue
Figure 10 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_4.jpg
Normal Tissue
Figure 11 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_5.jpg
Normal Tissue
Figure 12 Testis
Leydig cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Peritubular cells
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_6_6.jpg
Normal Tissue
Figure 13 Lymph node
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_8_8.jpg
RNA Expression
Figure 14 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141867-BRD4
❮
❯
Specifications
- Applications
- Immunoprecipitation-Mass Spectrometry
- Matrix
- Magrose bead (> 50 μmol/mL gel)
- Bead Ligand
- Anti-BRD4 specific antibody (D392)
- Target
- BRD4
- Immunogen
- Recombinant BRD4 protein
- Target Species
- Human
- Antibody Clone
- D392
- Bead Capacity
- 20-30 mg/mL binding antibody
- Bead size
- 10-37 μm
- Stability
- pH 2-14
- Format
- 20% Suspension
- Buffer
- PBS, pH 7.4, with 1% BSA
- Preservative
- 0.03% Proclin 300
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The protein encoded by this gene is homologous to the mouse protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
- Alternative Names
- BRD4; bromodomain containing 4; CAP; MCAP; HUNK1; HUNKI; bromodomain-containing protein 4; bromodomain-containing 4; chromosome-associated protein
- Gene ID
- 23476
- UniProt ID
- O60885
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0424-XY33. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "BRD4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-257 | Human Anti-BRD4 Recombinant Antibody (clone D242); Fab Fragment | IF, ELISA, IP-MS, ChIP | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-258 | Human Anti-BRD4 Recombinant Antibody (clone D392) | IF, ELISA, IP-MS | Human IgG1 |
| ZG-423C | Human Anti-BRD4 Recombinant Antibody (ZG-423C) | ELISA, IP, ChIP-qPCR | Human IgG1, κ |
| ZG-0573J | Rabbit Anti-BRD4 Recombinant Antibody (clone 1E5) | ELISA, WB, IHC | Rabbit IgG |
| VS3-XY3280 | Rabbit Anti-BRD4 Recombinant Antibody (clone R01-1H8) | WB, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS11-QX10 | Human Anti-BRD4 Recombinant Antibody (clone RAB-C131) | ELISA, IF, FC, IP, ChIP | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0384 | Hi-Affi™ Rabbit Anti-BRD4 Recombinant Antibody (clone DS384AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY261 | Anti-BRD4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY829 | Anti-Mouse BRD4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY828 | Anti-Human BRD4 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, WB
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, IHC, ELISA, FC
Application: WB, ELISA, FuncS, Inhib, PK, IP, SPR
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FuncS, Neut, IF, WB, EM, Inhib, IHC
Application: FC
Application: ELISA, Neut
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
