Anti-Human BRD4 Immunohistochemistry Kit
CAT#: VS-0525-XY828
Detect BRD4 efficiently with this high-performance IHC kit. Optimized for both frozen and FFPE tissues, it offers strong signal and low background, supporting studies on transcriptional control and cancer progression.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human skin
Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 5 IHC staining of human colon
Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68177/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 6 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 7 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/1080_H5_1_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_B_9_5.jpg
Normal Tissue
Figure 9 Colon
Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_3.jpg
Normal Tissue
Figure 10 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_4.jpg
Normal Tissue
Figure 11 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_5.jpg
Normal Tissue
Figure 12 Testis
Leydig cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Peritubular cells
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_6_6.jpg
Normal Tissue
Figure 13 Lymph node
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_8_8.jpg
RNA Expression
Figure 14 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141867-BRD4
❮
❯
Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human
- Target
- BRD4
- Primary Antibody
- Rabbit Anti-BRD4 Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-257 | Human Anti-BRD4 Recombinant Antibody (clone D242); Fab Fragment | IF, ELISA, IP-MS, ChIP | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-258 | Human Anti-BRD4 Recombinant Antibody (clone D392) | IF, ELISA, IP-MS | Human IgG1 |
| ZG-423C | Human Anti-BRD4 Recombinant Antibody (ZG-423C) | ELISA, IP, ChIP-qPCR | Human IgG1, κ |
| ZG-0573J | Rabbit Anti-BRD4 Recombinant Antibody (clone 1E5) | ELISA, WB, IHC | Rabbit IgG |
| VS3-XY3280 | Rabbit Anti-BRD4 Recombinant Antibody (clone R01-1H8) | WB, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS11-QX10 | Human Anti-BRD4 Recombinant Antibody (clone RAB-C131) | ELISA, IF, FC, IP, ChIP | Human IgG1, κ |
| CAT | Product Name | Application | Type |
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| MOR-0384 | Hi-Affi™ Rabbit Anti-BRD4 Recombinant Antibody (clone DS384AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS-0424-XY33 | AbPlus™ Anti-BRD4 Magnetic Beads (D392) | IP-MS, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY261 | Anti-BRD4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY829 | Anti-Mouse BRD4 Immunohistochemistry Kit | IHC |
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