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    Anti-Human BRD4 Immunohistochemistry Kit (CAT#: VS-0525-XY828)

    Detect BRD4 efficiently with this high-performance IHC kit. Optimized for both frozen and FFPE tissues, it offers strong signal and low background, supporting studies on transcriptional control and cancer progression.

    Specific Inquiry
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/if_selected.jpg
    Figure 2 IF staining of human cell line PC-3
    Figure 2 IF staining of human cell line PC-3
    (Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/if_selected.jpg
    Figure 3 IHC staining of human cerebellum
    Figure 3 IHC staining of human cerebellum
    (Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/ihc_selected.jpg
    Figure 4 IHC staining of human skin
    Figure 4 IHC staining of human skin
    (Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/ihc_selected.jpg
    Figure 5 IHC staining of human colon
    Figure 5 IHC staining of human colon
    (Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68177/ihc_selected.jpg
    Figure 6 IHC staining of human kidney
    Figure 6 IHC staining of human kidney
    (Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/ihc_selected.jpg
    Figure 7 IF staining of human cell line MCF7
    Figure 7 IF staining of human cell line MCF7
    (Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/1080_H5_1_red_green.jpg
    Normal Tissue
    Figure 8 Cerebral cortex
    Figure 8 Cerebral cortex
    (Endothelial cells
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Location: Nuclear
    Glial cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear
    Neuronal cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_B_9_5.jpg
    Figure 9 Colon
    Figure 9 Colon
    (Endothelial cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Glandular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_3.jpg
    Figure 10 Liver
    Figure 10 Liver
    (Cholangiocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Hepatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_4.jpg
    Figure 11 Kidney
    Figure 11 Kidney
    (Cells in glomeruli
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Cells in tubules
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_5.jpg
    Figure 12 Testis
    Figure 12 Testis
    (Leydig cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Pachytene spermatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Peritubular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Preleptotene spermatocytes
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Round or early spermatids
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Sertoli cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Spermatogonia cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_6_6.jpg
    Figure 13 Lymph node
    Figure 13 Lymph node
    (Non-germinal center cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_8_8.jpg
    RNA Expression
    Figure 14 RNA cell line category: Low cell line specificity
    Figure 14 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141867-BRD4

    Specifications

    • Application
    • IHC
    • Size
    • 50 Tests
    • Species Reactivity
    • Human
    • Target
    • BRD4
    • Primary Antibody
    • Rabbit Anti-BRD4 Antibody
    • Secondary Antibody
    • Goat anti-Rabbit Antibody, HRP
    • Sample Type
    • FFPE tissue; Frozen section tissue
    • Kit Storage
    • All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.

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    Fab Antibodies IgG Antibodies Rabbit Monoclonal Antibodies Antibody Magnetic Beads IHC Kits & Antibodies
    CAT Product Name Application Type
    DrMAB-257 Human Anti-BRD4 Recombinant Antibody (clone D242); Fab Fragment IF, ELISA, IP-MS, ChIP Human Fab

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