Rabbit Anti-BRD4 Recombinant Antibody (clone 1E5) (CAT#: ZG-0573J)
This product is a rabbit antibody that recognizes BRD4.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Rabbit Anti-BRD4 Antibody (ZG-0573J) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
All lanes: BRD4 antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 153, 81, 89 kDa
Observed band size: 153 kDa
IHC
Figure 2 Rabbit Anti-BRD4 Antibody (ZG-0573J) in IHC
IHC image of ZG-0573J diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IHC
Figure 3 Rabbit Anti-BRD4 Antibody (ZG-0573J) in IHC
IHC image of ZG-0573J diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human Brd4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human BRD4
- Species Reactivity
- Human
- Clone
- 1E5
- Applications
- ELISA, WB, IHC
- Conjugate
- Uconjugated
- Related Disease
- Cancer
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- CAP; MCAP; HUNK1; HUNKI; bromodomain-containing protein 4; chromosome-associated protein; mitotic chromosome-associated protein
- Gene ID
- 23476
- UniProt ID
- O60885
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Antibodies
IgG Antibodies
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-257 | Human Anti-BRD4 Recombinant Antibody (clone D242); Fab Fragment | IF, ELISA, IP-MS, ChIP | Human Fab |
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