1 0
Cart
    Inquiry
    Popular Searches: Recombinant Antibody MHC Tetramer T cell Receptor Scaffold Protein

    Rabbit Anti-BRD4 Recombinant Antibody (clone R01-1H8) (CAT#: VS3-XY3280)

    The product is a recombinant rabbit antibody that recognizes BRD4 (Bromodomain containing 4). It is capable of binding to human, mouse and rat BRD4. It has been used in a variety of applications, such as Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunocytochemistry or Immunofluorescence.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/if_selected.jpg
    Figure 2 IF staining of human cell line PC-3
    Figure 2 IF staining of human cell line PC-3
    (Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/if_selected.jpg
    Figure 3 IHC staining of human cerebellum
    Figure 3 IHC staining of human cerebellum
    (Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/ihc_selected.jpg
    Figure 4 IHC staining of human skin
    Figure 4 IHC staining of human skin
    (Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/ihc_selected.jpg
    Figure 5 IHC staining of human colon
    Figure 5 IHC staining of human colon
    (Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68177/ihc_selected.jpg
    Figure 6 IHC staining of human kidney
    Figure 6 IHC staining of human kidney
    (Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/ihc_selected.jpg
    Figure 7 IF staining of human cell line MCF7
    Figure 7 IF staining of human cell line MCF7
    (Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/1080_H5_1_red_green.jpg
    Normal Tissue
    Figure 8 Cerebral cortex
    Figure 8 Cerebral cortex
    (Endothelial cells
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Location: Nuclear
    Glial cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear
    Neuronal cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_B_9_5.jpg
    Figure 9 Colon
    Figure 9 Colon
    (Endothelial cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Glandular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_3.jpg
    Figure 10 Liver
    Figure 10 Liver
    (Cholangiocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Hepatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_4.jpg
    Figure 11 Kidney
    Figure 11 Kidney
    (Cells in glomeruli
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Cells in tubules
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_5.jpg
    Figure 12 Testis
    Figure 12 Testis
    (Leydig cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Pachytene spermatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Peritubular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Preleptotene spermatocytes
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Round or early spermatids
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Sertoli cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Spermatogonia cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_6_6.jpg
    Figure 13 Lymph node
    Figure 13 Lymph node
    (Non-germinal center cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_8_8.jpg
    RNA Expression
    Figure 14 RNA cell line category: Low cell line specificity
    Figure 14 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141867-BRD4

    Specifications

    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human, Mouse and Rat BRD4
    • Species Reactivity
    • Human, Mouse, Rat
    • Clone
    • R01-1H8
    • Applications
    • WB, IHC-P, ICC, IF, IP
    • Related Disease
    • Cornelia De Lange Syndrome

    Product Property

    • Clonality
    • Monoclonal
    • Aggregation
    • < 5%
    • Format
    • Liquid
    • Buffer
    • PBS (pH 7.4), 150 mM NaCl and 50% glycerol
    • Preservative
    • 0.02% sodium azide
    • Storage
    • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • WB: 1/500-1/1000
      IHC: 1/50-1/100
      IF: 1/50-1/200
      IP: 1/20

    Target

    • Alternative Names
    • CAP; MCAP; CDLS6; HUNK1; HUNKI; FSHRG4
    • Long Name
    • Bromodomain containing 4
    • Cellular Localization
    • Chromosome, Nucleus
    • Post Translation Modifications
    • Phosphorylation by CK2 disrupt the intramolecular binding between the bromo domain 2 and the NPS region and promotes binding between the NPS and the BID regions, leading to activate the protein and promote binding to acetylated histones.
    • Function
    • Regulates differentiation of naive CD4+ T-cells into T-helper Th17 by promoting recruitment of P-TEFb to promoters.
      Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II.
      Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters.

    Recommended Products

    Isotype Control

    Recommended Dilution Buffer

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "BRD4"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    Fab Antibody Products IgG Antibody Products Rabbit Monoclonal Antibody Products Antibody Magnetic Beads IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
    CAT Product Name Application Type
    DrMAB-257 Human Anti-BRD4 Recombinant Antibody (clone D242); Fab Fragment IF, ELISA, IP-MS, ChIP Human Fab

    Customer Reviews and Q&As

    Submit a review or a question

    There are currently no Customer reviews or questions for VS3-XY3280. Click the button above to contact us or submit your feedback about this product.

    Popular products with customers

    Figure 2 Anti-CD248 Recombinant Antibody (TAB-773) in HPLC

    Anti-CD248 Recombinant Antibody (Ontuxizumab) (CAT# TAB-773)
    Application: Neut, ELISA, IF, IP, FuncS, FC, ICC

    Figure 3 Anti-Human IL17RA Antibody (TAB-134) in DB

    Human Anti-IL17RA Recombinant Antibody (TAB-134) (CAT# TAB-134)
    Application: Neut, ELISA, IF, IP, FuncS, FC, ICC

    Figure 2 Anti-Human IL12+IL23 Recombinant Antibody (TAB-063) in HPLC

    Anti-Human IL12+IL23 Recombinant Antibody (Ustekinumab) (CAT# TAB-063)
    Application: WB, FuncS, IF, Neut, ELISA, FC, IP

    Figure 4 Human Anti-HIV-1 Env Recombinant Antibody (PABL-151) in ELISA

    Human Anti-HIV-1 Env Recombinant Antibody (clone PGDM1400) (CAT# PABL-151)
    Application: Neut, FuncS

    Figure 2 Anti-PF4 Recombinant Antibody (PABL-299) in HPLC

    Mouse Anti-PF4 Recombinant Antibody (clone KKO) (CAT# PABL-299)
    Application: ELISA, FuncS

    Figure 2 Anti-Human EGFR Antibody (PABW-088) in DB

    Mouse Anti-EGFR Recombinant Antibody (clone 7A7) (CAT# PABW-088)
    Application: ELISA, WB

    Figure 1 Recombinant Mouse Anti-UPAR Antibody (ATN615) (PABC-130) in WB

    Mouse Anti-UPAR Recombinant Antibody (clone ATN615) (CAT# PABC-130)
    Application: ELISA, IP, WB, IHC, IF, FuncS

    Figure 3 Mouse Anti-VEGF Recombinant Antibody (PABW-130) in ELISA

    Mouse Anti-VEGF Recombinant Antibody (clone A4.6.1) (CAT# PABW-130)
    Application: Neut, ELISA, Inhib, ICC, WB

    Figure 2 CCP Recombinant Antibody (TAB-390CQ) in HPLC

    Anti-Human CCP Recombinant Antibody (F12) (CAT# TAB-390CQ)
    Application: ELISA, IHC

    Figure 3 Anti-Human SLC1A5 Recombinant Antibody (TAB-1009CLV) in FC

    Anti-Human SLC1A5 Recombinant Antibody (TAB-1009CLV) (CAT# TAB-1009CLV)
    Application: FC, ADCC, CDC, Inhib

    Figure 2 Anti-SARS-CoV S Recombinant Antibody (MRO-654LC) in HPLC

    Mouse Anti-SARS-CoV S Recombinant Antibody (MRO-654LC) (CAT# MRO-654LC)
    Application: ELISA

    Figure 2 Anti-Human F8 Antibody in HPLC

    Recombinant Human Anti-F8 Antibody (BO2C11) (CAT# PABX-058)
    Application: WB, ELISA, FuncS, Inhib, PK, IP, SPR

    Figure 1 Anti-Human CD40 Agonist Antibody (MOB-107LC) in ELISA

    Human Anti-CD40 Recombinant Antibody (MOB-107LC) (CAT# MOB-107LC)
    Application: FC, FuncS, ELISA

    Figure 5 Human Anti-FAP Recombinant Antibody (HPAB-0175-CN) in ELISA

    Human Anti-FAP Recombinant Antibody (clone ESC14) (CAT# HPAB-0175-CN)
    Application: ELISA, WB, FC, IHC, IP

    Figure 4 Human Anti-TGFBR2 Recombinant Antibody (HPAB-0150-FY) in DB

    Human Anti-TGFBR2 Recombinant Antibody (clone LY3022859) (CAT# HPAB-0150-FY)
    Application: ELISA

    Figure 5 Recombinant Human Anti-HCV E2 Antibody (HPAB-0196CQ) in Dot Blot

    Recombinant Human Anti-HCV E2 Antibody (HPAB-0196CQ) (CAT# HPAB-0196CQ)
    Application: ELISA, Neut

    Figure 2 Recombinant Human Anti-LAM Antibody (PABC-542) in HPLC

    Recombinant Human Anti-LAM Antibody (PABC-542) (CAT# PABC-542)
    Application: ELISA, WB

    Figure 3 Anti-LAM Recombinant Antibody (clone A194-01); Fab Fragment (HPAB-M0560-YC-F(E)) in SDS-PAGE

    Human Anti-LAM Recombinant Antibody; Fab Fragment (HPAB-M0560-YC-F(E)) (CAT# HPAB-M0560-YC-F(E))
    Application: ELISA, WB

    Figure 5 Human Anti-EBV gB Recombinant Antibody (Clone AMMO2) (PABS-0037) in DB

    Human Anti-EBV gB Recombinant Antibody (Clone AMMO2) (CAT# PABS-0037)
    Application: ELISA, FuncS

    Figure 5 Human Anti-EBV gB Recombinant Antibody (Clone AMMO5) (PABS-0040) in DB

    Human Anti-EBV gB Recombinant Antibody (Clone AMMO5) (CAT# PABS-0040)
    Application: ELISA, Neut, FuncS

    For Research Use Only. Not For Clinical Use.

    Send Inquiry

    This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

    Go to compare

    Go to compare