Rabbit Anti-BRD4 Recombinant Antibody (clone R01-1H8) (CAT#: VS3-XY3280)
The product is a recombinant rabbit antibody that recognizes BRD4 (Bromodomain containing 4). It is capable of binding to human, mouse and rat BRD4. It has been used in a variety of applications, such as Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunocytochemistry or Immunofluorescence.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/if_selected.jpg

(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/if_selected.jpg

(Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/ihc_selected.jpg

(Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/ihc_selected.jpg

(Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68177/ihc_selected.jpg

(Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/ihc_selected.jpg

(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/1080_H5_1_red_green.jpg

(Endothelial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_B_9_5.jpg

(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_3.jpg

(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_4.jpg

(Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_5.jpg

(Leydig cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Peritubular cells
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_6_6.jpg

(Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_8_8.jpg

(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141867-BRD4
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat BRD4
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-1H8
- Applications
- WB, IHC-P, ICC, IF, IP
- Related Disease
- Cornelia De Lange Syndrome
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- CAP; MCAP; CDLS6; HUNK1; HUNKI; FSHRG4
- Gene ID
- 23476
- UniProt ID
- O60885
- Long Name
- Bromodomain containing 4
- Cellular Localization
- Chromosome, Nucleus
- Post Translation Modifications
- Phosphorylation by CK2 disrupt the intramolecular binding between the bromo domain 2 and the NPS region and promotes binding between the NPS and the BID regions, leading to activate the protein and promote binding to acetylated histones.
- Protein Refseq
- XP_047294497.1; XP_047294496.1
- Function
- Regulates differentiation of naive CD4+ T-cells into T-helper Th17 by promoting recruitment of P-TEFb to promoters.
Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II.
Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "BRD4"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
DrMAB-257 | Human Anti-BRD4 Recombinant Antibody (clone D242); Fab Fragment | IF, ELISA, IP-MS, ChIP | Human Fab |
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For Research Use Only. Not For Clinical Use.
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