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    Rabbit Anti-BRD4 Recombinant Antibody (clone R01-1H8) (CAT#: VS3-XY3280)

    The product is a recombinant rabbit antibody that recognizes BRD4 (Bromodomain containing 4). It is capable of binding to human, mouse and rat BRD4. It has been used in a variety of applications, such as Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunocytochemistry or Immunofluorescence.

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    • Conjugation:

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    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/if_selected.jpg
    Figure 2 IF staining of human cell line PC-3
    Figure 2 IF staining of human cell line PC-3
    (Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/if_selected.jpg
    Figure 3 IHC staining of human cerebellum
    Figure 3 IHC staining of human cerebellum
    (Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/ihc_selected.jpg
    Figure 4 IHC staining of human skin
    Figure 4 IHC staining of human skin
    (Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/ihc_selected.jpg
    Figure 5 IHC staining of human colon
    Figure 5 IHC staining of human colon
    (Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68177/ihc_selected.jpg
    Figure 6 IHC staining of human kidney
    Figure 6 IHC staining of human kidney
    (Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/ihc_selected.jpg
    Figure 7 IF staining of human cell line MCF7
    Figure 7 IF staining of human cell line MCF7
    (Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/1080_H5_1_red_green.jpg
    Normal Tissue
    Figure 8 Cerebral cortex
    Figure 8 Cerebral cortex
    (Endothelial cells
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Location: Nuclear
    Glial cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear
    Neuronal cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_B_9_5.jpg
    Figure 9 Colon
    Figure 9 Colon
    (Endothelial cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Glandular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_3.jpg
    Figure 10 Liver
    Figure 10 Liver
    (Cholangiocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Hepatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_4.jpg
    Figure 11 Kidney
    Figure 11 Kidney
    (Cells in glomeruli
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Cells in tubules
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_5.jpg
    Figure 12 Testis
    Figure 12 Testis
    (Leydig cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Pachytene spermatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Peritubular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Preleptotene spermatocytes
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Round or early spermatids
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Sertoli cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Spermatogonia cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_6_6.jpg
    Figure 13 Lymph node
    Figure 13 Lymph node
    (Non-germinal center cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_8_8.jpg
    RNA Expression
    Figure 14 RNA cell line category: Low cell line specificity
    Figure 14 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141867-BRD4

    Specifications

    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human, Mouse and Rat BRD4
    • Species Reactivity
    • Human, Mouse, Rat
    • Clone
    • R01-1H8
    • Applications
    • WB, IHC-P, ICC, IF, IP
    • Related Disease
    • Cornelia De Lange Syndrome

    Product Property

    • Clonality
    • Monoclonal
    • Aggregation
    • < 5%
    • Format
    • Liquid
    • Buffer
    • PBS (pH 7.4), 150 mM NaCl and 50% glycerol
    • Preservative
    • 0.02% sodium azide
    • Storage
    • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • WB: 1/500-1/1000
      IHC: 1/50-1/100
      IF: 1/50-1/200
      IP: 1/20

    Target

    • Alternative Names
    • CAP; MCAP; CDLS6; HUNK1; HUNKI; FSHRG4
    • Long Name
    • Bromodomain containing 4
    • Cellular Localization
    • Chromosome, Nucleus
    • Post Translation Modifications
    • Phosphorylation by CK2 disrupt the intramolecular binding between the bromo domain 2 and the NPS region and promotes binding between the NPS and the BID regions, leading to activate the protein and promote binding to acetylated histones.
    • Function
    • Regulates differentiation of naive CD4+ T-cells into T-helper Th17 by promoting recruitment of P-TEFb to promoters.
      Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II.
      Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters.

    Recommended Products

    Isotype Control

    Recommended Dilution Buffer

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    CAT Product Name Application Type
    DrMAB-257 Human Anti-BRD4 Recombinant Antibody (clone D242); Fab Fragment IF, ELISA, IP-MS, ChIP Human Fab

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