Popular Searches: Recombinant Antibody MHC Tetramer T cell Receptor Scaffold Protein
  • Home
  • BRD4
  • Rabbit Anti-BRD4 Recombinant Antibody (clone R01-1H8)

Rabbit Anti-BRD4 Recombinant Antibody (clone R01-1H8) (CAT#: VS3-XY3280)

The product is a recombinant rabbit antibody that recognizes BRD4 (Bromodomain containing 4). It is capable of binding to human, mouse and rat BRD4. It has been used in a variety of applications, such as Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunocytochemistry or Immunofluorescence.


See more Engineered Antibody products for BRD4
Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/if_selected.jpg
Figure 2 IF staining of human cell line PC-3
Figure 2 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/if_selected.jpg
Figure 3 IHC staining of human cerebellum
Figure 3 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/ihc_selected.jpg
Figure 4 IHC staining of human skin
Figure 4 IHC staining of human skin
(Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/ihc_selected.jpg
Figure 5 IHC staining of human colon
Figure 5 IHC staining of human colon
(Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68177/ihc_selected.jpg
Figure 6 IHC staining of human kidney
Figure 6 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/ihc_selected.jpg
Figure 7 IF staining of human cell line MCF7
Figure 7 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/1080_H5_1_red_green.jpg
Normal Tissue
Figure 8 Cerebral cortex
Figure 8 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_B_9_5.jpg
Figure 9 Colon
Figure 9 Colon
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_3.jpg
Figure 10 Liver
Figure 10 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_4.jpg
Figure 11 Kidney
Figure 11 Kidney
(Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_5.jpg
Figure 12 Testis
Figure 12 Testis
(Leydig cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Peritubular cells
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_6_6.jpg
Figure 13 Lymph node
Figure 13 Lymph node
(Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_8_8.jpg
RNA Expression
Figure 14 RNA cell line category: Low cell line specificity
Figure 14 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141867-BRD4

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat BRD4
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R01-1H8
  • Applications
  • WB, IHC-P, ICC, IF, IP
  • Related Disease
  • Cornelia De Lange Syndrome

Product Property

  • Clonality
  • Monoclonal
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl and 50% glycerol
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • WB: 1/500-1/1000
    IHC: 1/50-1/100
    IF: 1/50-1/200
    IP: 1/20

Target

  • Alternative Names
  • CAP; MCAP; CDLS6; HUNK1; HUNKI; FSHRG4
  • Long Name
  • Bromodomain containing 4
  • Cellular Localization
  • Chromosome, Nucleus
  • Post Translation Modifications
  • Phosphorylation by CK2 disrupt the intramolecular binding between the bromo domain 2 and the NPS region and promotes binding between the NPS and the BID regions, leading to activate the protein and promote binding to acetylated histones.
  • Function
  • Regulates differentiation of naive CD4+ T-cells into T-helper Th17 by promoting recruitment of P-TEFb to promoters.
    Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II.
    Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters.

Recommended Products

Isotype Control

Recommended Dilution Buffer

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "BRD4"

Fab Fragment Antibody

CAT Product Name Application Type
DrMAB-257 Human Anti-BRD4 Recombinant Antibody (clone D242); Fab Fragment IF, ELISA, IP-MS, ChIP Human Fab

Recombinant Antibody

CAT Product Name Application Type
DrMAB-258 Human Anti-BRD4 Recombinant Antibody (clone D392) IF, ELISA, IP-MS Human IgG1
ZG-423C Human Anti-BRD4 Recombinant Antibody (ZG-423C) ELISA, IP, ChIP-qPCR Human IgG1, κ
ZG-0573J Rabbit Anti-BRD4 Recombinant Antibody (clone 1E5) ELISA, WB, IHC Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0384 Hi-Affi™ Rabbit Anti-BRD4 Recombinant Antibody (clone DS384AB) WB, IHC, ICC Rabbit IgG

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for VS3-XY3280. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart
    Inquiry

    Go to compare