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    Hi-Affi™ Rabbit Anti-BRD4 Recombinant Antibody (clone DS384AB) (CAT#: MOR-0384)

    Recombinant Rabbit anti-BRD4 monoclonal antibody for WB, IHC, ICC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/if_selected.jpg
    Figure 2 IF staining of human cell line PC-3
    Figure 2 IF staining of human cell line PC-3
    (Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/if_selected.jpg
    Figure 3 IHC staining of human cerebellum
    Figure 3 IHC staining of human cerebellum
    (Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/ihc_selected.jpg
    Figure 4 IHC staining of human skin
    Figure 4 IHC staining of human skin
    (Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/ihc_selected.jpg
    Figure 5 IHC staining of human colon
    Figure 5 IHC staining of human colon
    (Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68177/ihc_selected.jpg
    Figure 6 IHC staining of human kidney
    Figure 6 IHC staining of human kidney
    (Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/ihc_selected.jpg
    Figure 7 IF staining of human cell line MCF7
    Figure 7 IF staining of human cell line MCF7
    (Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/1080_H5_1_red_green.jpg
    Normal Tissue
    Figure 8 Cerebral cortex
    Figure 8 Cerebral cortex
    (Endothelial cells
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Location: Nuclear
    Glial cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear
    Neuronal cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_B_9_5.jpg
    Figure 9 Colon
    Figure 9 Colon
    (Endothelial cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Glandular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_3.jpg
    Figure 10 Liver
    Figure 10 Liver
    (Cholangiocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Hepatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_4.jpg
    Figure 11 Kidney
    Figure 11 Kidney
    (Cells in glomeruli
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Cells in tubules
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_5.jpg
    Figure 12 Testis
    Figure 12 Testis
    (Leydig cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Pachytene spermatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Peritubular cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Preleptotene spermatocytes
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Round or early spermatids
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Sertoli cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Spermatogonia cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_6_6.jpg
    Figure 13 Lymph node
    Figure 13 Lymph node
    (Non-germinal center cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_8_8.jpg
    RNA Expression
    Figure 14 RNA cell line category: Low cell line specificity
    Figure 14 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141867-BRD4

    Specifications

    • Host Species
    • Rabbit
    • Derivation
    • Phage display library screening
    • Type
    • Rabbit IgG
    • Species Reactivity
    • Human
    • Clone
    • DS384AB
    • Applications
    • WB, IHC, ICC
    • Conjugate
    • Unconjugated

    Product Property

    • Purification
    • Purified with Absolute Mag™ Protein A Agarose
    • Purity
    • >95% as determined by analysis by SDS-PAGE
    • Format
    • Liquid
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Target

    • Alternative Names
    • Bromodomain Containing 4; Chromosome-Associated Protein; Bromodomain-Containing 4; HUNK1; Protein HUNK1; HUNKI; MCAP; CAP

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    CAT Product Name Application Type
    DrMAB-257 Human Anti-BRD4 Recombinant Antibody (clone D242); Fab Fragment IF, ELISA, IP-MS, ChIP Human Fab

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