Hi-Affi™ Rabbit Anti-BRD4 Recombinant Antibody (clone DS384AB) (CAT#: MOR-0384)
Recombinant Rabbit anti-BRD4 monoclonal antibody for WB, IHC, ICC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/if_selected.jpg

(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/if_selected.jpg

(Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/ihc_selected.jpg

(Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/ihc_selected.jpg

(Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68177/ihc_selected.jpg

(Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/ihc_selected.jpg

(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/1080_H5_1_red_green.jpg

(Endothelial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_B_9_5.jpg

(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_3.jpg

(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_4.jpg

(Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_5.jpg

(Leydig cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Peritubular cells
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_6_6.jpg

(Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_8_8.jpg

(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141867-BRD4
Specifications
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Human
- Clone
- DS384AB
- Applications
- WB, IHC, ICC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Bromodomain Containing 4; Chromosome-Associated Protein; Bromodomain-Containing 4; HUNK1; Protein HUNK1; HUNKI; MCAP; CAP
- Gene ID
- 23476
- UniProt ID
- O60885
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "BRD4"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
DrMAB-257 | Human Anti-BRD4 Recombinant Antibody (clone D242); Fab Fragment | IF, ELISA, IP-MS, ChIP | Human Fab |
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For Research Use Only. Not For Clinical Use.
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