Human Anti-BRD4 Recombinant Antibody (ZG-423C) (CAT#: ZG-423C)

Name: Human Anti-BRD4 Recombinant Antibody (ZG-423C)
SKU: ZG-423C
Rating: 5 (1 reviews)

This product is a human antibody that recognizes BRD4. It can be used for ELISA, IP and ChIP-qPCR.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

ELISA

Figure 1 Human Anti-BRD4 Antibody (ZG-423C) in ELISA

Figure 1 Human Anti-BRD4 Antibody (ZG-423C) in ELISA

Antibody was tested on human BRD4 and WDR5 (non-target).

Subcellular Location and Protein Expression

Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/if_selected.jpg

Figure 2 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/if_selected.jpg

Figure 3 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15055/ihc_selected.jpg

Figure 4 IHC staining of human skin
(Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/ihc_selected.jpg

Figure 5 IHC staining of human colon
(Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68177/ihc_selected.jpg

Figure 6 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/ihc_selected.jpg

Figure 7 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61646/1080_H5_1_red_green.jpg

Normal Tissue

Figure 8 Cerebral cortex
(Endothelial cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_B_9_5.jpg

Figure 9 Colon
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_3.jpg

Figure 10 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_4.jpg

Figure 11 Kidney
(Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_7_5.jpg

Figure 12 Testis
(Leydig cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Peritubular cells
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_6_6.jpg

Figure 13 Lymph node
(Non-germinal center cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68178/152034_A_8_8.jpg

RNA Expression

Figure 14 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000141867-BRD4

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Human bromodomain-containing protein 4 isoform long(333-460 of 1362)

Host Species
Human

Type
Human IgG1, κ

Specificity
Specificity has been tested in ELISA and IP-MS and ChIP-qPCR.

Species Reactivity
Human

Applications
ELISA, IP, ChIP-qPCR

Product Property

Purification
Protein A affinity purified

Purity
>95% as deterimined by SDS-PAGE

Format
Liquid

Buffer
PBS

Storage
Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.

Applications

Application Notes
ELISA 1: 25000. IP 2 µg/ml

Target

Alternative Names
Bromodomain Containing 4; Chromosome-Associated Protein; HUNK1; Bromodomain-Containing Protein 4; Bromodomain-Containing 4; Protein HUNK1; HUNKI; MCAP; CAP;

Gene ID
23476

UniProt ID
O60885

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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DrMAB-257	Human Anti-BRD4 Recombinant Antibody (clone D242); Fab Fragment	IF, ELISA, IP-MS, ChIP	Human Fab

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CAT	Product Name	Application	Type
DrMAB-258	Human Anti-BRD4 Recombinant Antibody (clone D392)	IF, ELISA, IP-MS	Human IgG1
ZG-0573J	Rabbit Anti-BRD4 Recombinant Antibody (clone 1E5)	ELISA, WB, IHC	Rabbit IgG
VS3-XY3280	Rabbit Anti-BRD4 Recombinant Antibody (clone R01-1H8)	WB, IHC-P, ICC, IF, IP	Rabbit IgG
VS11-QX10	Human Anti-BRD4 Recombinant Antibody (clone RAB-C131)	ELISA, IF, FC, IP, ChIP	Human IgG1, κ