Mouse Anti-Btk Recombinant Antibody (ZG-0353F)
CAT#: ZG-0353F
This product is a mouse antibody that recognizes Btk.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-Btk Recombinant Antibody (ZG-0353F) in WB.
Western blot analysis was performed using Btk monoclonal antibodies against K562 (1), MCF-7 (2), Jurkat (3) and HEK293 (4) cell lysates.
IHC
Figure 2 Mouse Anti-Btk Recombinant Antibody (ZG-0353F) in IHC.
Immunohistochemical analysis of paraffin-embedded human lymph node tissue (left) and human lymph follicular tissue (right), showing cytoplasmic and membrane localization by DAB staining using Btk monoclonal antibody.
IF
Figure 3 Mouse Anti-Btk Recombinant Antibody (ZG-0353F) in IF.
The Btk monoclonal antibody was used for immunofluorescence analysis of Jurkat cells.
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Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2028/1770_B11_1_selected.jpg
Normal Tissue
Figure 2 Colon
Enterocytes - Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_A_9_3.jpg
Normal Tissue
Figure 3 High expression in lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_A_8_8.jpg
Normal Tissue
Figure 4 Spleen
Cells in red pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in white pulp Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1198/2770_B_8_4.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (Daudi, HEL, HMC-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000010671-BTK
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Specifications
- Immunogen
- Recombinant fragment of Btk expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human Btk
- Species Reactivity
- Human, Monkey
- Applications
- Western Blot, Immunohistochemistry, Immunofluorescence, Enzyme-linked Immunosorbent Assay
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Immunohistochemistry: 1/200-1/1000
Immunofluorescence: 1/200-1/1000
ELISA: 1/10000
Target
- Alternative Names
- Bruton Tyrosine Kinase; Bruton Agammaglobulinemia Tyrosine Kinase; B-Cell Progenitor Kinase; Brutons Tyrosine Kinase; EC 2.7.10.2; AGMX1; ATK; BPK; Tyrosine-Protein Kinase BTK Isoform (Lacking Exon 13 To 17); Dominant-Negative Kinase-Deficient Brutons Tyrosine Kinase; Tyrosine-Protein Kinase BTK Isoform (Lacking Exon 14); BTK; AGMX1; ATK; BPK; Tyrosine-protein kinase BTK; Agammaglobulinaemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase
- Gene ID
- 695
- UniProt ID
- Q06187
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Related Signaling Pathways
BCR Signaling Pathway
Related Diseases
Primary Immunodeficiency
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MOB-1492MZ | Mouse Anti-BTK Recombinant Antibody (clone 4j6) | IP, WB | Mouse IgG3 |
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| ZG-0354F | Mouse Anti-BTK Recombinant Antibody (ZG-0354F) | WB | Mouse IgG |
| VS3-WK2119 | Rabbit Anti-BTK (pY223) Recombinant Antibody (clone R09-4V4) | WB, IP | Rabbit IgG |
| VS3-WK2141 | Rabbit Anti-BTK (pY551) Recombinant Antibody (clone R07-9C1) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0393 | Hi-Affi™ Rabbit Anti-BTK Recombinant Antibody (clone DS393AB) | ICC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY34 | AbPlus™ Anti-BTK Magnetic Beads (CBACN-075) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY57 | Mouse Anti-NHP BTK Recombinant Antibody (clone 7F12H4) | WB, IHC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY267 | Anti-BTK Immunohistochemistry Kit | IHC | |
| VS-0525-XY854 | Anti-Mouse BTK Immunohistochemistry Kit | IHC | |
| VS-0525-XY853 | Anti-Human BTK Immunohistochemistry Kit | IHC |
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