AbPlus™ Anti-CA9 Magnetic Beads (12F10)
CAT#: VS-0424-XY37
The AbPlus Anti-CA9 Magnetic Beads (12F10) is an innovative affinity resin which is bound with anti-CA9 specific antibody (12F10). The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of CA9 protein under native and denaturing conditions.
Gene Expression
Normal Tissue
Figure 1 Immunofluorescent staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5100/639_D5_2_selected.jpg
Normal Tissue
Figure 2 Stomach
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55207/139851_A_5_1.jpg
RNA Expression
Figure 3 RNA cell line category: Group enriched (A-431, HDLM-2, hTERT-RPE1, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000107159-CA9
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magrose bead (> 50 μmol/mL gel)
- Bead Ligand
- Anti-CA9 specific antibody (12F10)
- Target
- CA9
- Immunogen
- Synthetic peptide of CA9
- Target Species
- Human
- Antibody Clone
- 12F10
- Bead Capacity
- 20-30 mg/mL binding antibody
- Bead size
- 10-37 μm
- Stability
- pH 2-14
- Format
- 20% Suspension
- Buffer
- PBS, pH 7.4, with 1% BSA
- Preservative
- 0.03% Proclin 300
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.
- Alternative Names
- MN; CAIX; carbonic anhydrase 9; CA-IX; P54/58N; RCC-associated antigen G250; RCC-associated protein G250; carbonate dehydratase IX; carbonic anhydrase IX; carbonic dehydratase; membrane antigen MN; pMW1; renal cell carcinoma-associated antigen G250
- Gene ID
- 768
- UniProt ID
- Q16790
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1276z | Mouse Anti-CA9 Recombinant Antibody (clone 21C7) | ELISA, FC, IHC, WB | Mouse IgG1, κ |
| HPAB-1174WJ | Mouse Anti-CA9 Recombinant Antibody (clone AB-MN-21.5.2) | ADCC | Mouse IgG1 |
| HPAB-1175WJ | Mouse Anti-CA9 Recombinant Antibody (clone AB-MN-21.6.1) | ADCC | Mouse IgG1 |
| ZG-0735F | Mouse Anti-CA9 Recombinant Antibody (clone 66.4.C2) | IHC-P, IHC-Fr | Mouse IgG2b |
| ZG-0578J | Rabbit Anti-CA9 Recombinant Antibody (clone 4F12) | ELISA, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-707 | Anti-CAIX/CA9 Recombinant Antibody (Girentuximab) | WB, FuncS, IF, Neut, ELISA, FC, IP | IgG1 - kappa |
| TAB-H43 | Anti-Human CA9 Recombinant Antibody (TAB-H43) | ELISA, IP, IF, Inhib | IgG1 - kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-017 | Mouse Anti-CA9 Recombinant Antibody (clone M75); Fab Fragment | WB, FuncS | Mouse IgG2b |
| HPAB-0104-LSX-F(E) | Human Anti-CA9 Recombinant Antibody; Fab Fragment (HPAB-0104-LSX-F(E)) | ELISA | Human Fab |
| HPAB-0105-LSX-F(E) | Human Anti-CA9 Recombinant Antibody; Fab Fragment (HPAB-0105-LSX-F(E)) | ELISA | Human Fab |
| HPAB-0782-CN-F(E) | Human Anti-CA9 Recombinant Antibody (clone G119); Fab Fragment | ELISA, FC | Human Fab |
| HPAB-0784-CN-F(E) | Human Anti-CA9 Recombinant Antibody (clone G37); Fab Fragment | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-017 | Mouse Anti-CA9 Recombinant Antibody (clone M75); scFv Fragment | WB, FuncS | Mouse scFv |
| HPAB-0104-LSX-S(P) | Human Anti-CA9 Recombinant Antibody; scFv Fragment (HPAB-0104-LSX-S(P)) | ELISA | Human scFv |
| HPAB-0105-LSX-S(P) | Human Anti-CA9 Recombinant Antibody; scFv Fragment (HPAB-0105-LSX-S(P)) | ELISA | Human scFv |
| HPAB-1174WJ-S(P) | Mouse Anti-CA9 Recombinant Antibody (clone AB-MN-21.5.2); scFv Fragment | ELISA | Mouse scFv |
| HPAB-1175WJ-S(P) | Mouse Anti-CA9 Recombinant Antibody (clone AB-MN-21.6.1); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1454CL-F(E) | Anti-Human CA9 Recombinant Antibody Fab Fragment (G250) | Cyt | |
| TAB-1455CL-F(E) | Anti-Human CA9 Recombinant Antibody Fab Fragment (S239D/I332E) | SPR | |
| TAB-1456CL-F(E) | Anti-Human CA9 Recombinant Antibody Fab Fragment (MSC1) | ELISA, FC, Inhib | Fully human antibody |
| TAB-1457CL-F(E) | Anti-Human CA9 Recombinant Antibody Fab Fragment (MSC2) | ELISA, FC, Inhib | Fully human antibody |
| TAB-1459CL-F(E) | Anti-Human CA9 Recombinant Antibody Fab Fragment (MSC4) | ELISA, FC, Inhib | Fully human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0427 | Hi-Affi™ Rabbit Anti-CA9 Recombinant Antibody (clone DS427AB) | IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H43 | Afuco™ Anti-CA9 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H43) | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1029 | AbPlus™ Anti-CA9 Magnetic Beads (VS-0724-YC1029) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY54 | Human Anti-CA9 (clone G37) scFv-Fc Chimera | FC, ELISA, Inhib | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY290 | Anti-CA9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY911 | Anti-Mouse CA9 Immunohistochemistry Kit | IHC | |
| VS-0525-XY912 | Anti-Human CA9 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC94 | Recombinant Anti-CA9 Vesicular Antibody, EV Displayed (VS-0425-YC94) | ELISA, FC, Neut, Cell-uptake |
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