Mouse Anti-CD14 Recombinant Antibody (clone 14G1F3) (CAT#: ZG-0242U)
Mouse anti-CD14 antibody (clone 14G1F3) displayed high specificity against the CD14 protein of Human, Mouse and Rabbit.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Mouse Anti-CD14 Antibody (ZG-0242U) in WB
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate All lanes: CD14 antibody at 1: 1800
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 55 kDa
IHC
Figure 2 Mouse Anti-CD14 Antibody (ZG-0242U) in IHC
IHC image of ZG-0242U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Mouse Anti-CD14 Antibody (ZG-0242U) in IF
Immunofluorescence staining of A549 cells with ZG-0242U at 1:90, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).
FC
Figure 4 Mouse Anti-CD14 Antibody (ZG-0242U) in FC
Overlay histogram showing Jurkat cells stained with ZG-0242U (red line) at 1:180. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.
Specifications
- Immunogen
- Recombinant Human Monocyte differentiation antigen CD14 protein (20-345AA)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CD14
- Species Reactivity
- Human, Mouse, Rabbit
- Clone
- 14G1F3
- Applications
- ELISA, WB, IHC, IF, FC
- Conjugate
- Unconjugated
- Related Disease
- Mycobacterium Chelonae and Mycobacterium Kansasii.
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry. Specific conditions should be optimized by the end user.
WB:1:1000-1:5000, IHC:1:50-1:200, IF:1:50-1:200
Target
- Alternative Names
- CD14 molecule; monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
- Gene ID
- 929
- UniProt ID
- P08571
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "CD14"
Select a product category from the dropdown menu below to view related products.
Please select product type
Human Antibody Products
Mouse Antibody Products
Chimeric Antibody Products
Chicken IgY Antibody Products
Neutralizing Antibody Products
Blocking Antibody Products
Rabbit Monoclonal Antibody Products
ScFv Antibody Products
Fab Antibody Products
IgG Antibody Products
Agonistic Antibody Products
Non-Human Primate (NHP) Antibody Products
Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
ScFv-Fc Chimera Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1589CL | Anti-Human CD14 Recombinant Antibody (scFv2F9) | WB, FC, Block | Human antibody |
| TAB-1589CL-S(P) | Anti-Human CD14 Recombinant Antibody scFv Fragment (scFv2F9) | WB, FC, Block | Human antibody |
| TAB-1589CL-F(E) | Anti-Human CD14 Recombinant Antibody Fab Fragment (scFv2F9) | WB, FC, Block | Human antibody |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for ZG-0242U. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: WB, Neut, FuncS
Application: WB, ELISA, FuncS
-2.jpg)
Application: IB, ELISA, FC, FuncS
Application: Neut, Inhib, FC, ELISA
Application: WB, IHC, ELISA, FC
Application: Neut, ELISA, FuncS
Application: WB, ELISA, IHC, IP, SPR, FuncS
-2.png)
Application: ELISA
Application: ELISA, Neut, FuncS
Application: ELISA, WB
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
