Mouse Anti-CD14 Recombinant Antibody (clone 1A7C5) (CAT#: ZG-0243U)
Mouse anti-CD14 antibody (clone 1A7C5) displayed high specificity against the CD14 protein of Human, Mouse, Rat and Rabbit.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Mouse Anti-CD14 Antibody (ZG-0243U) in WB
Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate All lanes: CD14 antibody at 1: 1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 41 kDa
Observed band size: 55 kDa
IHC
Figure 2 Mouse Anti-CD14 Antibody (ZG-0243U) in IHC
IHC image of ZG-0243U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Mouse Anti-CD14 Antibody (ZG-0243U) in IF
Immunofluorescence staining of HepG2 cells with ZG-0243U at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).
Specifications
- Immunogen
- Recombinant Human Monocyte differentiation antigen CD14 protein (20-345AA)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CD14
- Species Reactivity
- Human, Mouse, Rat, Rabbit
- Clone
- 1A7C5
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Mycobacterium Chelonae and Mycobacterium Kansasii.
Product Property
- Purification
- Protein A purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:1000-1:64000, IHC:1:50-1:200, IF:1:50-1:200
Target
- Alternative Names
- CD14 molecule; monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
- Gene ID
- 929
- UniProt ID
- P08571
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1182z | Mouse Anti-CD14 Recombinant Antibody (clone 13B10) | ICC, IF, FuncS | Mouse IgG1 |
| HPAB-0101-YC | Mouse Anti-CD14 Recombinant Antibody (clone 28C5) | ELISA, FC, Block, FuncS | Mouse IgG1 |
| ZG-0079J | Mouse Anti-CD14 Recombinant Antibody (ZG-0079J) | IHC-P, IF | Mouse IgG |
| ZG-0080J | Mouse Anti-CD14 Recombinant Antibody (ZG-0080J) | IHC-P, IF | Mouse IgG |
| ZG-0837F | Mouse Anti-CD14 Recombinant Antibody (clone FWKW) | FC | Mouse IgG2b |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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