Anti-CD276 Antibody Prodrug, Protease Activated (VS-1025-YC101)
CAT#: VS-1025-YC101
The Anti-CD276 Antibody Prodrug, Protease Activated (VS-1025-YC101) is an antibody prodrug comprised of a recombinant human antibody targeting human CD276, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to CD276 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to CD276 effectively. CD276, also known as B7-H3, is a member of the B7 family of immune checkpoint ligands that is broadly overexpressed across many solid tumors and hematological cancers. Its function is primarily inhibitory to T-cells, promoting immune evasion and tumor growth. This widespread expression makes CD276 an attractive target for novel antibody therapies and CAR T-cell approaches.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17139/if_selected.jpg
Normal Tissue
Figure 2 Placenta.
High expression in placenta
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17826/40943_A_1_7_val_selected.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_5_6.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_7_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HaCaT, U-2197)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103855-CD276
❮
❯
Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human CD276
- Target
- CD276
- Species Reactivity
- Human, Mouse
- Isotype
- IgG
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
- Alternative Names
- CD276 Molecule; CD276 Antigen; B7 Homolog 3; 4Ig-B7-H3; B7-H3; B7H3; B7RP-2
- Gene ID
- 80381
- UniProt ID
- Q5ZPR3
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Datasheet
MSDS
COA
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See other products for "CD276"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-117CL | Anti-Human CD276 Recombinant Antibody (TAB-117CL) | ADCC, FuncS | Antibody |
| TAB-424CQ | Anti-Human CD276 Recombinant Antibody (TAB-424CQ) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1603MZ | Mouse Anti-CD276 Recombinant Antibody (clone NJI53) | FC | Mouse IgG1 |
| HPAB-0234-CN | Human Anti-CD276 Recombinant Antibody (HPAB-0234-CN) | ELISA, FC, IF | Human IgG |
| HPAB-0236-CN | Mouse Anti-CD276 Recombinant Antibody (HPAB-0236-CN) | IHC, Cyt, Inhib, ELISA, FC | Mouse IgG1, κ |
| HPAB-0237-CN | Mouse Anti-CD276 Recombinant Antibody (HPAB-0237-CN) | IHC, ELISA, FC | Mouse IgG1, κ |
| HPAB-0238-CN | Mouse Anti-CD276 Recombinant Antibody (HPAB-0238-CN) | ELISA, FC, IHC | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-333CQ | Rat Anti-CD276 Recombinant Antibody (clone MJ18) | Block, FC | Rat IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0549 | Hi-Affi™ Rabbit Anti-CD276 Recombinant Antibody (clone DS549AB) | WB, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0234-CN-S(P) | Human Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-0234-CN-S(P)) | ELISA, FC, IF | Human scFv |
| HPAB-0236-CN-S(P) | Mouse Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-0236-CN-S(P)) | ELISA, FC, IHC | Mouse scFv |
| HPAB-0237-CN-S(P) | Mouse Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-0237-CN-S(P)) | ELISA, FC, IHC | Mouse scFv |
| HPAB-0238-CN-S(P) | Mouse Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-0238-CN-S(P)) | ELISA, FC, IHC | Mouse scFv |
| HPAB-0239-CN-S(P) | Human Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-0239-CN-S(P)) | ELISA, FC, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0234-CN-F(E) | Human Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-0234-CN-F(E)) | ELISA, FC, IF | Human Fab |
| HPAB-0236-CN-F(E) | Mouse Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-0236-CN-F(E)) | ELISA, FC, IHC | Mouse Fab |
| HPAB-0237-CN-F(E) | Mouse Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-0237-CN-F(E)) | ELISA, FC, IHC | Mouse Fab |
| HPAB-0238-CN-F(E) | Mouse Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-0238-CN-F(E)) | ELISA, FC, IHC | Mouse Fab |
| HPAB-0239-CN-F(E) | Human Anti-CD276 Recombinant Antibody; Fab Fragment (HPAB-0239-CN-F(E)) | ELISA, FC, IHC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-424CQ | Afuco™ Anti-CD276 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-424CQ) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1279 | AbPlus™ Anti-Cd276 Magnetic Beads (VS-0724-YC1279) | IP, Protein Purification | |
| VS-0724-YC1280 | AbPlus™ Anti-Cd276 Magnetic Beads (VS-0724-YC1280) | IP, Protein Purification | |
| VS-0724-YC1366 | AbPlus™ Anti-CD276 Magnetic Beads (VS-0724-YC1366) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY67 | Human Anti-CD276 (clone MGA271) scFv-Fc Chimera | ADCC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC156 | Recombinant Anti-CD276 Vesicular Antibody, EV Displayed (VS-0425-YC156) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1155 | Anti-CD276 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1156 | Anti-Mouse CD276 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC48 | SmartAb™ Recombinant Anti-CD276 pH-dependent Antibody (VS-0825-YC48) | ELISA, IHC, FC, IP, IF | Human IgG1 kappa |
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