Mouse Anti-CD276 Recombinant Antibody; scFv Fragment (HPAB-0237-CN-S(P)) (CAT#: HPAB-0237-CN-S(P))
This HPAB-0237-CN product is a recombinant mouse IgG1, κ antibody that binds human B7-H3. The anti-B7-H3 antibody was found to be able to bind Hs 700T cells (+++). HPAB-0237-CN-S(P) antibody is the scfv fragment antibody form of HPAB-0237-CN antibody. Single-chain variable fragments (scFvs) are artificial constructs consisting of the variable regions of the immunoglobulin heavy and light chains connected by a peptide linker.
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Custom Production
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17139/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17139/if_selected.jpg
Figure 2 Placenta.
(High expression in placenta)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17826/40943_A_1_7_val_selected.jpg
(High expression in placenta)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17826/40943_A_1_7_val_selected.jpg
Figure 3 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_5_6.jpg
(Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_5_6.jpg
Figure 4 Cerebral cortex
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_B_7_5.jpg
(Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_B_7_5.jpg
Figure 5 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_9_3.jpg
Figure 6 Liver
(Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_7_4.jpg
(Cholangiocytes Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9285/25731_A_7_4.jpg
Figure 7 RNA cell line category: Cell line enhanced (HaCaT, U-2197)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103855-CD276
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000103855-CD276
Specifications
- Immunogen
- The antibody was produced by immunizing mice with immunogenic amounts of cells, cell extracts, or protein preparations containing human B7-H3.
- Host Species
- Mouse
- Type
- Mouse scFv
- Specificity
- Human CD276
- Species Reactivity
- Human, Cynomolgus
- Applications
- ELISA, FC, IHC
- Related Disease
- Cancer and inflammation
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry: 0.0195 μg/mL-0.156 μg/mL
Target
- Alternative Names
- CD276 Molecule; Costimulatory Molecule; CD276 Antigen; B7 Homolog 3; 4Ig-B7-H3; B7-H3; B7H3; B7RP-2
- Gene ID
- 80381
- UniProt ID
- Q5ZPR3
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-117CL | Anti-Human CD276 Recombinant Antibody (TAB-117CL) | ADCC, FuncS | Antibody |
| TAB-424CQ | Anti-Human CD276 Recombinant Antibody (TAB-424CQ) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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