Recombinant llama Anti-CEACAM5 Antibody (HPAB-0204-FY) (CAT#: HPAB-0204-FY)
This product is a recombinant antibody that recognizes Human CEACAM5. The monoclonal antibody 11C12 reacts with the epitope of CEACAM5.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HaCaT
(Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19758/1660_G5_2_selected.jpg
(Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19758/1660_G5_2_selected.jpg
Figure 2 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11041/ihc_selected.jpg
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11041/ihc_selected.jpg
Figure 3 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Gradient
Gradient: Ascending
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_7_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Gradient
Gradient: Ascending
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_7_3.jpg
Figure 4 Rectum
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_6_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21/19_A_6_3.jpg
Figure 5 RNA cell line category: Cell line enriched (OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105388-CEACAM5
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105388-CEACAM5
Figure 5 RNA cell line category: Cell line enriched (OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105388-CEACAM5
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105388-CEACAM5
Specifications
- Immunogen
- llama immunized: a healthy and adult alpacas, recombinant CEA protein and Freund's adjuvant 1: 1 mixing ratio, using subcutaneous injection at multiple sites by way immune 6-7yg / Kg alpaca, a total of four times immunization, immunization at intervals of 2 weeks. After collection the peripheral blood alpaca, for construction of phage display libraries.
- Host Species
- llama
- Type
- llama VHH
- Specificity
- Human CEACAM5
- Species Reactivity
- Human
- Clone
- HPAB-0204-FY
- Applications
- ADCC, ELISA
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- The CEACAM5 antibody has been reported in applications of Antibody-dependent Cell-mediated Cytotoxicity, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- Carcinoembryonic Antigen Related Cell Adhesion Molecule 5; Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5; Meconium Antigen 100; CEA; Carcinoembryonic Antigen; CD66e Antigen; CD66e
- Gene ID
- 1048
- UniProt ID
- P06731
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-080 | Recombinant Anti-human CEACAM5 VHH Single Domain Antibody | WB, ELISA, IHC, FC, FuncS | Llama VHH |
| TAB-002LC-VHH | Anti-Human CEACAM5 Recombinant Antibody(VHH library) | SPR, FC, ELISA | Single domain antibody |
| HPAB-0205-FY | Recombinant llama Anti-CEACAM5 Antibody (HPAB-0205-FY) | ADCC, E | llama VHH |
| HPAB-0206-FY | Recombinant llama Anti-CEACAM5 Antibody (HPAB-0206-FY) | ADCC, E | llama VHH |
| HPAB-1024WJ | Recombinant Camelid Anti-CEACAM5 Single Domain Antibody | SPR, Inhib | Camelid VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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